Time-kill kinetics and biocidal effect of Euclea crispa leaf extracts against microbial membrane

Alayande, Kazeem Adekunle; Pohl, Carolina H.; Ashafa, Anofi Omotayo Tom

doi:10.1016/j.apjtm.2017.03.022

Cited by 16 publications

(12 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The maximum amount of proteins (0.566 µg/ml) was released from K. pneumoniae by an n-butanol fraction at 2×MIC after 120 min of treatment while the maximum amount of nucleotides released (4.575 µg) was from B. pumilus by n-hexane fraction under similar condition. Furthermore, membrane disruption has also been confirmed in the course of this study as one of the mechanisms of biocidal action of the leaf extract [81].…”

Section: Cell Membrane Disruptionsupporting

confidence: 72%

“…Hence, leaf extracts of E. crispa could be a good source of readily available drug against the aforementioned ailments in folklore remedy. The growth of E. coli, a common causative agent of diarrhea and urinary tract infections, and species of Salmonella and Shigella were also significantly inhibited by the extracts [81]. These findings validate traditional use of E. crispa as herbal medicine against antimicrobial infections.…”

Section: Antifungal Activitiessupporting

confidence: 61%

“…Pretorius et al [79] [81]. These findings somehow confirm the species' antibacterial potential and its usefulness in the treatment of stomach disorders [15,41,45,47], toothache [51], venereal diseases [12], and other microbial infections [15,45,49].…”

Section: Antibacterial Activitiesmentioning

confidence: 71%

“…The killing rate by the potent fractions was determined using B. pumilus, K. pneumoniae, and C. albicans as representative isolates. Alayande et al [81] evaluated the effects of E. crispa leaf extracts on cell membrane disruption by determining the amount of proteins and nucleotides released from within the cells and scanning electron microscopy images of the membrane after 120 min of treatment. The fraction partitioned into n-butanol achieved absolute mortality against B. pumilus and K. pneumoniae after 90 and 120 min contact time, respectively, at 1×MIC.…”

Section: Cell Membrane Disruptionmentioning

confidence: 99%

See 3 more Smart Citations

Euclea Crispa: Review of Its Botany, Ethnomedicinal Uses, and Pharmacological Properties

Maroyi

2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Euclea crispa is widely used as herbal medicine in southern Africa. This study was aimed at reviewing the botany, ethnomedicinal uses, and validated pharmacological properties of E. crispa. The literature search for information on ethnomedicinal uses and pharmacological activities of E. crispa was undertaken using databases such as web of science, BMC, science direct, elsevier, scopus, PubMed, and scielo. Other relevant literature sources included books, book chapters, websites, theses, conference papers, and other scientific publications. The extensive literature survey revealed that the bark, fruits, leaves, and roots of E. crispa are commonly used as herbal medicines for wounds, constipation, cough, stomach disorders, epilepsy, rheumatism, and diabetes. Pharmacological studies on E. crispa indicate that the species has amyloid β-peptide lowering effects, antibacterial, antidiarrheal, antifungal, and cell membrane disruption activities. E. crispa should be subjected to further scientific evaluations aimed at elucidating its chemical, pharmacological, and toxicological properties. Such detailed research should also include experimental animal studies, randomized clinical trials, and target-organ toxicity studies involving E. crispa extracts and its derivatives.

show abstract

Section: Cell Membrane Disruptionsupporting

confidence: 72%

Section: Antifungal Activitiessupporting

confidence: 61%

Section: Antibacterial Activitiesmentioning

confidence: 71%

Section: Cell Membrane Disruptionmentioning

confidence: 99%

See 2 more Smart Citations

Euclea Crispa: Review of Its Botany, Ethnomedicinal Uses, and Pharmacological Properties

Maroyi

2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

show abstract

“…It is widespread and common in the interior regions of southern Africa. 6 This plant is used traditionally against wide range of ailments such as gonorrhea, leprosy, scabies, diarrhea and wound infections and previous reports have shown that the plant possessed anti-bacterial and antifungal activities. 7 Virtual screening based on computer-aided techniques are very promising in drug discovery and plays an important role in identifying lead compounds from natural products.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Novel C-X-C Chemokine Receptor Type 4 (CXCR4) Inhibitors from Hexane Extract of Euclea crispa (Thunb.) Leaves by Chemical Fingerprint Identification and Molecular Docking Analysis

Palanisamy¹,

Ashafa²

2018

JYP

View full text Add to dashboard Cite

Objectives: To evaluate the potential inhibitors aginst CXCR4 from hexane extract of Euclea crispa (E. crispa) leaves. Methods: The natural compounds were identified and charecterized through GC-MS Analysis. Molecular docking studies carried out to investigatethe inhibitory activity angainst CXCR4. Absorption, Distribution, Metabolism, and Excretion (ADME) properties also were predicted to find pharmacokinetics and pharmacodynamics of compounds.Results:The molecular docking simulations revealed that, Benzoic acid 3-methyl-4-(1,3,3,3-tetrafluoro-2-methoxycarbonyl-propenylsulfanyl)-phenyl ester (SCHEMBL15979821), Hydrocortisone Acetate and Phenyl Glucuronide possess good inhibitory activites (Glide Score of -7.06, -6.97, -6.47 and Glide Energy of -43.22, -48.27 and -32.80 kcal/mol respectively) when compared with standard FDA approved drug and other compounds. All the screened compounds were within the acceptable and permissible limits of ADME properties. Conclusion: Thus, from this study it can be concluded that, these screened natural compounds from E. crispa leaves may serve as potential inhibitors for CXCR4 and they might lead to development of new therapeutic agents against cancer and its associated complications.Key words: E.crispa, GC-MS analysis, CXCR4, Molecular docking, ADME properties. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. Correspondence

show abstract

Deciphering the antimicrobial, antibiofilm and membrane stabilizing synergism of Mikania scandens (L.) Willd. leaves and stems substantiation through in vitro and in silico studies

Tumpa

Chowdhury

Asma

2023

Biotechnology Reports

View full text Add to dashboard Cite

Time-kill kinetics and biocidal effect of Euclea crispa leaf extracts against microbial membrane

Abstract: This study suggests the leaf of Euclea crispa a source of bioactive compound with membrane attack as one of the mechanisms of its biocidal action.

Cited by 16 publications

References 15 publications

Euclea Crispa: Review of Its Botany, Ethnomedicinal Uses, and Pharmacological Properties

Euclea Crispa: Review of Its Botany, Ethnomedicinal Uses, and Pharmacological Properties

Analysis of Novel C-X-C Chemokine Receptor Type 4 (CXCR4) Inhibitors from Hexane Extract of Euclea crispa (Thunb.) Leaves by Chemical Fingerprint Identification and Molecular Docking Analysis

Deciphering the antimicrobial, antibiofilm and membrane stabilizing synergism of Mikania scandens (L.) Willd. leaves and stems substantiation through in vitro and in silico studies

Contact Info

Product

Resources

About