Time-dependent observation of individual cellular binding events to field-effect transistors

Schäfer, Susanne; Eick, Stefan; Hofmann, Boris; Dufaux, T.; Stockmann, Regina; Wrobel, Günter; Offenhäusser, Andreas; Ingebrandt, Sven

doi:10.1016/j.bios.2008.07.003

Cited by 52 publications

(50 citation statements)

References 34 publications

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“…Amplifier System and Data Acquisition: The amplifier system used for the recording of cell signals was a previously published design [42][43][44]. It offered the possibility of electrical characterization of transistors and timedependent potentiometric readout of drain-source current changes of 16 channels.…”

Section: Methodsmentioning

confidence: 99%

Diamond Transistor Array for Extracellular Recording From Electrogenic Cells

Dankerl

Eick

Hofmann

et al. 2009

Adv Funct Materials

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The transduction of electric signals from cells to electronic devices is mandatory for medical applications such as neuroprostheses and fundamental research on communication in neuronal networks. Here, the use of diamond with its advantages for biological applications as a new material for biohybrid devices for the detection of cell signals is investigated. Using the surface conductivity of hydrogen‐terminated single‐crystalline diamond substrates, arrays of solution‐gate field‐effect transistors were fabricated. The characterization of the transistors reveals a good stability in electrolyte solutions for at least 7 days. On these devices, cardiomyocyte‐like HL‐1 cells as well as human embryonic kidney cells (HEK293), which were stably transfected with potassium channels, are cultured. Both types of cells show healthy growth and good adhesion to the substrate. The diamond transistors are used to detect electrical signals from both types of cells by recording the extracellular potential. For the HL‐1 cells, the shape of action potentials can be resolved and the propagation of the signal across the cell layer is visible. Potassium currents of HEK293 cells are activated with the patch‐clamp technique in voltage‐clamp mode and simultaneously measured with the field‐effect transistors. The ion sensitivity of the diamond surface enables the detection of released potassium ions accumulated in the cleft between transistor and cell.

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Section: Methodsmentioning

confidence: 99%

Diamond Transistor Array for Extracellular Recording From Electrogenic Cells

Dankerl

Eick

Hofmann

et al. 2009

Adv Funct Materials

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show abstract

“…The electronic characterization of the devices was performed using a Keithley 4200-SCS parameter analyzer (Tektronix Inc., USA) and a home-made, portable 16 channel amplifier system, which was described in former publications of our group (Ingebrandt et al 2007;Ingebrandt et al 2005;Schäfer et al 2009). An Ag/AgCl wire was used in the measurement medium as pseudo reference electrode.…”

Section: Characterization Setupmentioning

confidence: 99%

PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells

Hempel

Law

Nguyen

et al. 2017

Biosensors and Bioelectronics

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“…Thus, seal resistances and coupling qualities could not be determined for this prototype (see supplemental material). In future experiments with biohybrid sensors, impedance spectroscopy could be used to estimate the seal resistance of cells without any patch-clamp contact (Fromherz et al, 1999;Kiessling et al, 2000;Schäfer et al, 2009). Tightening the contact between cells and electronics leads to higher signal amplitudes.…”

Section: Limits Improvements and Capabilities Of Cell-based Biohybrimentioning

confidence: 99%

Extracellular recording of glycine receptor chloride channel activity as a prototype for biohybrid sensors

Sommerhage

Baumann

Wrobel

et al. 2010

Biosensors and Bioelectronics

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Time-dependent observation of individual cellular binding events to field-effect transistors

Cited by 52 publications

References 34 publications

Diamond Transistor Array for Extracellular Recording From Electrogenic Cells

Diamond Transistor Array for Extracellular Recording From Electrogenic Cells

PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells

Extracellular recording of glycine receptor chloride channel activity as a prototype for biohybrid sensors

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