1999
DOI: 10.1152/ajpcell.1999.277.6.c1044
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Abstract: Time-dependent changes in myosin heavy chain (MHC) isoform expression were investigated in rat soleus muscle unloaded by hindlimb suspension. Changes at the mRNA level were measured by RT-PCR and correlated with changes in the pattern of MHC protein isoforms. Protein analyses of whole muscle revealed that MHCI decreased after 7 days, when MHCIIa had increased, reaching a transient maximum by 15 days. Longer periods led to inductions and progressive increases of MHCIId(x) and MHCIIb. mRNA analyses of whole musc… Show more

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Cited by 101 publications
(96 citation statements)
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“…The suspension duration was chosen because of significant modifications of soleus wet weight [40] and vascular adaptation [28] without any modification of body weight.…”
Section: Hindlimb Unloadingmentioning
confidence: 99%
“…The suspension duration was chosen because of significant modifications of soleus wet weight [40] and vascular adaptation [28] without any modification of body weight.…”
Section: Hindlimb Unloadingmentioning
confidence: 99%
“…Moreover, mammalian skeletal muscle fibers display a great potential of adaptation that results from the ability of muscle fibers to adjust their molecular, functional, and metabolic properties in response to altered functional demands, such as changes in neuromuscular activity or mechanical loading (28). Indeed, we have previously shown slow-to-fast functional transitions induced by hindlimb unloading (HU) that encompassed slow-to-fast transitions in the isoform composition of myosin heavy chain (29) as well as in other key proteins involved in the muscle contraction (30,31). These transitions were associated with aerobic-oxidative to glycolytic metabolic changes (32) that involved both variations in the glucose metabolism and phosphorylation/dephosphorylation events (33,34).…”
mentioning
confidence: 99%
“…As recently shown for rat soleus muscle unloaded by hindlimb suspension, the slowto-fast transition in MHC mRNA and protein isoforms occur in the order (25) : MHCIβ → MHCIIa → MHCIId(x) → MHCIIb. The MHC mRNA was reverse-transcribed with the use of specifi c oligonucleotide primers, followed by amplifi cation in the polymerase chain reaction (PCR), yielding DNA fragments of defined length (25). The purpose of this study is to evaluate the effects of magnetic stimulation in preventing acute muscle atrophy.…”
mentioning
confidence: 51%
“…Numerous studies using rats have shown that mechanical unloading of soleus muscle induces slowto-fast transitions (19,27). Stevens et al (25) reported that MHCIβ, the predominant isoform in normal soleus muscle (~95%), decreased within the fi rst week of unloading, reaching a relative concentration of ~75% at day 7. MHCIIa, which represented 5% of total MHC isoforms in control soleus muscle, was elevated by the muscle unloading and reached a maximum of ~25% after 1 week.…”
Section: Discussionmentioning
confidence: 99%
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