Time course of various cell origin circulating microparticles in ST-segment elevation myocardial infarction patients undergoing percutaneous transluminal coronary intervention

Abstract

Abstract. The present study aimed to investigate the time course of changes in microparticles (MPs) in patients with ST-segment elevation myocardial infarction (STEMI) that underwent percutaneous transluminal coronary intervention (PCI). A total of 24 STEMI patients undergoing primary PCI were enrolled, and circulating MPs were detected immediately prior to and after PCI, and at 4, 24 and 48 h post-PCI. Standard Megamix beads, based measurement protocols, were employed to measure MPs of different cell origin, including endothelial MPs (EMPs), platelet MPs (PMPs) and leukocyte-derived MPs (LMPs), which were identified by CD144, CD41 and CD45, respectively. The results indicated that PMP levels were evidently elevated immediately after PCI, and reached a maximum level at 48 h. In addition, LMP and EMP levels were significantly decreased immediately after the PCI, and then increased gradually with time. The total quantity of the three aforementioned MP types increased gradually at 48 h following PCI. Furthermore, coronary angiographic Gensini scores were significantly positively correlated with the level of PMPs (r 2 =0.42; P=0.0006). Log-normalized high sensitivity-C-reactive-protein was also significantly correlated with LMPs (r 2 =0.86; P<0.01). In conclusion, the time course of the changes in circulating MPs of different cell origin, provided information on possible functions of different MPs in STEMI.

Molecular Mechanisms Underpinning Microparticle-Mediated Cellular Injury in Cardiovascular Complications Associated with Diabetes

“…Furthermore, it has been reported that the numbers of circulating MPs continued to increase in the short term following appropriate intervention in STEMI patients. Zhou et al [117] followed up circulating levels of MPs over time in STEMI patients undergoing percutaneous transluminal coronary intervention (PCI) (before and up to 48 h after PCI). The authors found that platelet MPs increased directly after the PCI and continued to rise over time until the end of follow-up period, whereas the levels of leukocyte- and endothelial-derived MPs decreased after the PCI, but rose thereafter until the end of the follow-up period [117].…”

Microparticles and cardiovascular diseases

“…In the setting of acute coronary syndromes, the most studied microparticles are platelet derived microparticles and endothelial derived microparticles. Observational studies showed increased levels of CD146+ and CD31+ endothelial derived microparticles ( microparticles decreased immediately post PCI and gradually increased up to 48 hours later (264). Abciximab, an GPIIb-IIIa antagonist, in combination with primary coronary angioplasty reduces the level of platelet derived microparticles compared with patients that had only percutaneous angioplasty (261).…”