Tildipirosin: An effective antibiotic against Glaesserella parasuis from an in vitro analysis

Peres, Priscila Rodrigues; Prigol, Simone Ramos; Gutiérrez-Martín, César B.; Feronatod, César; Suriñach, Miquel Collell; Kreutz, Luiz Carlos; Frandoloso, Rafael

doi:10.1016/j.vas.2020.100136

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…Due to the high efficacy of tildipirosin, especially against A. pleuropneumoniae (Table 1) and G. parasuis [15], we decided to evaluate the capacity of this antibiotic to eliminate the colonization of bacteria related to PRDC and that belong to the Pasteurellaceae family. A. pleuropneumoniae and P. multocida serogroup A are two important microorganisms responsible for lung disease in pigs during the growing-finishing phase, resulting in substantial economic losses related to clinical outbreaks, as well as for condemnations during the inspection of carcasses at slaughterhouses [1,10].…”

Section: Discussionmentioning

confidence: 99%

“…The antimicrobial susceptibility to tildipirosin was determined using the broth microdilution method in accordance with the recommendations by the Clinical and Laboratory Standards Institute [44,45], with slight modification. Colonies from each A. pleuropneumoniae or P. multocida clinical isolate were picked and homogenized in demineralized water, quantified by flow cytometry [15], and diluted in Veterinary Fastidious Medium (for A. pleuropneumoniae) or cation-adjusted Mueller-Hinton broth (in the case of P. multocida) to reach an inoculum concentration of 5 × 10 5 bacteria/mL. Different Tildipirosin concentrations (ranging from 0.06 to 64 µg/mL) in 96-well plates were incubated with 100 µL per well of the bacteria inoculum at 37 • C for 18-20 h, under a 5-10% CO 2 atmosphere for A. pleuropneumoniae, only.…”

Section: Antimicrobial Sensitivity Testingmentioning

confidence: 99%

“…Tildipirosin was developed by MSD Animal Health and introduced in international markets under the name Zuprevo in 2011 [14]. This antibiotic, available only as injectable presentation, has high in vitro efficacy against A. pleuropneumoniae, G. parasuis and Pasteurella multocida [15][16][17], and represents an excellent weapon for the treatment of these important infectious agents.…”

Section: Introductionmentioning

confidence: 99%

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Brazilian Clinical Strains of Actinobacillus pleuropneumoniae and Pasteurella multocida: Capsular Diversity, Antimicrobial Susceptibility (In Vitro) and Proof of Concept for Prevention of Natural Colonization by Multi-Doses Protocol of Tildipirosin

Kuchiishi,

Ramos Prigol,

Bresolin

et al. 2023

Antibiotics

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One hundred Actinobacillus pleuropneumoniae (App) and sixty Pasteurella multocida subsp. multocida serogroup A (PmA) isolates were recovered from porcine pneumonic lungs collected from eight central or southern states of Brazil between 2014 and 2018 (App) or between 2017 and 2021 (PmA). A. pleuropneumoniae clinical isolates were typed by multiplex PCR and the most prevalent serovars were 8, 7 and 5 (43, 25% and 18%, respectively). In addition, three virulence genes were assessed in P. multocida isolates, all being positive to capA (PmA) and kmt1 genes, all negative to capD and toxA, and most of them (85%) negative to pfhA gene. The susceptibility of both pathogens to tildipirosin was investigated using a broth microdilution assay. The percentage of isolates susceptible to tildipirosin was 95% for App and 73.3% for PmA. The MIC50 values were 0.25 and 1 μg/mL and the MIC90 values were 4 and >64 μg/mL for App and PmA, respectively. Finally, a multiple-dose protocol of tildipirosin was tested in suckling piglets on a farm endemic for both pathogens. Tildipirosin was able to prevent the natural colonization of the tonsils by App and PmA and significantly (p < 0.0001) reduced the burden of Glaesserella parasuis in this tissue. In summary, our results demonstrate that: (i) tildipirosin can be included in the list of antibiotics to control outbreaks of lung disease caused by App regardless of the capsular type, and (ii) in the case of clinical strains of App and PmA that are sensitive to tildipirosin based on susceptibility testing, the use of this antibiotic in eradication programs for A. pleuropneumoniae and P. multocida can be strongly recommended.

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Section: Discussionmentioning

confidence: 99%

Section: Antimicrobial Sensitivity Testingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Brazilian Clinical Strains of Actinobacillus pleuropneumoniae and Pasteurella multocida: Capsular Diversity, Antimicrobial Susceptibility (In Vitro) and Proof of Concept for Prevention of Natural Colonization by Multi-Doses Protocol of Tildipirosin

Kuchiishi,

Ramos Prigol,

Bresolin

et al. 2023

Antibiotics

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Concerns about the use of antimicrobials in swine herds and alternative trends

Rigueira,

Perecmanis

2024

Translational Animal Science

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Pig productivity in Brazil has advanced a lot in recent decades. Specialized breeds are more vulnerable to pathogens, which has boosted the use of antimicrobials by farmers. The selective pressure generated favors the emergence of resistant bacteria, which compromises the effectiveness of this treatment and limits therapeutic options. In addition to increasing costs and mortality rates in the production system, public awareness of this issue has increased. The authorities have imposed restrictive measures to control the use of antimicrobials and have banned their use as growth promoters. This literature review highlights biosecurity and animal welfare to prevent pig diseases. Hence, we describe alternatives to the use of antimicrobials in pig production for the selection of effective non-antibiotic feed additives that help maintain good health and help the pig resist disease when infection occurs.

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Tildipirosinin Sığır Trakea Epitel Hücresi Üzerinde Yara İyileştirici Etkisi

Şahin

Yildirim

Ekici

et al. 2021

Harran Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu çalışmada; sığır solunum yolu hastalıkları tedavisinde kullanılan tildipirosinin, sığır trakea epitel hücresi üzerinde in vitro yara iyileşmesi etkisinin araştırılması amaçlandı. In vitro yara yöntemi ile her kuyucuğa ekilmiş olan sığır trakea epitel hücreleri üzerinde, 200 μL'lik pipet ucu (dikey) aracılığıyla bir yara alanı oluşturuldu. In vitro yara oluşturulmuş sığır trakea epitel hücreleri üzerine tildipirosinin konsantrasyonları (5 μg/mL, 10 μg/mL, 25 μg/mL, 50 μg/mL) ve kontrol grubu uygulandı. In vitro yara görüntüleri; 0'ıncı saat, 24'üncü saat ve 48'inci saatte izlendi. Çalışmanın 24'üncü saatinde yara alanı kapanma yüzdesi, kontrol grubuna kıyasla; tildipirosinin 50 μg/mL konsantrasyonda istatistiki olarak önemli fark bulunamazken 5, 10 ve 25 μg/mL konsantrasyonlarında istatistiki olarak fark anlamlı bulundu. Çalışmanın 48'inci saatinde, kontrol grubuna kıyasla 25 ve 50 μg/mL tildipirosin konsantrasyonları arasında istatistiki olarak önemli fark bulunamazken, 5 μg/mL (%96,61±1,69) ve 10 μg/mL (%96,42±1,78) tildipirosin konsantrasyonları arasında yara alanı kapanma yüzdeleri arasında istatistiki olarak fark anlamlı bulundu. Sonuç olarak tildipirosinin düşük konsantrasyonlarındaki yara iyileşme yüzdelerinin (48'inci saat) yaklaşık olarak %96 olması, hastalıkların tedavisine ek olarak çeşitli mikroorganizma ve maddeler tarafından epitel hücrelerinde meydana gelen hasarlara karşı doku bütünlüğünü koruma ve tedaviye katkı sağlayabilir.

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Tildipirosin: An effective antibiotic against Glaesserella parasuis from an in vitro analysis

Abstract: Highlights Glaesserella parasuis is highly susceptible to Tildipirosin. Tildipirosin is recommended for treating Glässer's disease outbreaks. MIC is the rational tool to survey the resistance of G. parasuis to tildipirosin.

Cited by 3 publications

References 16 publications

Brazilian Clinical Strains of Actinobacillus pleuropneumoniae and Pasteurella multocida: Capsular Diversity, Antimicrobial Susceptibility (In Vitro) and Proof of Concept for Prevention of Natural Colonization by Multi-Doses Protocol of Tildipirosin

Brazilian Clinical Strains of Actinobacillus pleuropneumoniae and Pasteurella multocida: Capsular Diversity, Antimicrobial Susceptibility (In Vitro) and Proof of Concept for Prevention of Natural Colonization by Multi-Doses Protocol of Tildipirosin

Concerns about the use of antimicrobials in swine herds and alternative trends

Tildipirosinin Sığır Trakea Epitel Hücresi Üzerinde Yara İyileştirici Etkisi

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