Tight Junction Transmembrane Protein Claudin Subtype Expression and Distribution in Human Corneal and Conjunctival Epithelium

Yoshida, Yusuke; Ban, Yuriko; Kinoshita, Shigeru

doi:10.1167/iovs.08-3046

Cited by 69 publications

(60 citation statements)

References 36 publications

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“…They are the source of the gel mucin of the tears (MUC5AC) (Argüeso and Gipson, 2001). The tight junctions of the conjunctival epithelium are more leaky than those of the corneal epithelium, possibly, as noted, because of differences in the number and type of claudin subunits that they contain (Yoshida et al, 2009). In keeping with this, the paracellular channels demonstrated by electron microscopy are much wider than those of the corneal epithelium (Bron et al, 1997;Huang et al, 1989).…”

Section: Tight Junctionsmentioning

confidence: 95%

“…Yoshida et al have summarized their role as structural components of Tjs in the corneal and the bulbar conjunctival epithelia (Yoshida et al, 2009). Despite the differences in permeability between these two epithelia they found that each epithelium expressed the same claudin subtypes, namely claudins 1, 4 and 7 throughout their thickness, although the expression of type 7 was superficial in the conjunctival epithelium.…”

Section: Tight Junctionsmentioning

confidence: 99%

See 1 more Smart Citation

Clinical staining of the ocular surface: Mechanisms and interpretations

Bron

Argüeso

İrkeç

et al. 2015

Progress in Retinal and Eye Research

135

109

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Section: Tight Junctionsmentioning

confidence: 95%

Section: Tight Junctionsmentioning

confidence: 99%

Clinical staining of the ocular surface: Mechanisms and interpretations

Bron

Argüeso

İrkeç

et al. 2015

Progress in Retinal and Eye Research

135

109

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“…Such a continuous permeability barrier functions through the formation of tight junctions that mediate adhesion between adjacent epithelial cells. 1 Tight junctions are composed of transmembrane proteins, including occludin, claudin, and a number of peripheral membrane structural proteins, such as zonula occludens (ZO), and are associated with a variety of regulatory proteins. 2 Disruption of these junctions is thought to perturb the normal cellular environment and lead to numerous pathological situations such as dry eye, asthma, and inflammatory bowel disease.…”

mentioning

confidence: 99%

EMMPRIN Modulates Epithelial Barrier Function through a MMP–Mediated Occludin Cleavage

Huet

Vallée

Delbé

et al. 2011

The American Journal of Pathology

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Dry eye is a common disease that develops as a result of alteration of tear fluid, leading to osmotic stress and a perturbed epithelial barrier. Matrix metalloproteinase-9 (MMP-9) may be important in dry eye disease, as its genetic knockout conferred resistance to the epithelial disruption. We show that extracellular matrix metalloproteinase inducer (EMMPRIN; also termed CD147), an inducer of MMP expression, participates in the pathogenesis of dry eye through MMPmediated cleavage of occludin, an important component of tight junctions. EMMPRIN expression was increased on the ocular surface of dry eye patients and correlated with those of MMP-9. High osmolarity in cell culture, mimicking dry eye conditions, increased both EMMPRIN and MMP-9 and resulted in the disruption of epithelial junctions through the cleavage of occludin. Exogenously added recombinant EMMPRIN had similar effects that were abrogated in the presence of the MMP inhibitor marimastat. Membrane occludin immunostaining was markedly increased in the apical corneal epithelium of both EMMPRIN and MMP-9 knock-out mice. Furthermore, an inverse correlation between EMMPRIN and occludin membrane staining was consistently observed both in vitro and in vivo as a function of corneal epithelial cells differentiation. These data suggest a possible role of EMMPRIN in regulating the amount of occludin at the cell surface in homeostasis beyond pathological situations such as dry eye disease, and EMMPRIN may be essential for the formation and maintenance of organized epithelial structure. (Am J Pathol

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“…Tight junctions are multifunctional and dynamic protein complexes that connect adjacent cells by sealing the intercellular space between cells preventing the paracellular movement of solutes, ions and water. The main proteins forming a tight junction in the cornea are occludin (Langbein et al, 2002), claudins (Ban et al, 2003) and zonula occludens-1 (ZO-1) (Ban et al, 2003;Sosnova-Netukova et al, 2007;Yoshida et al, 2009). It was possible to measure the effect of Ap 4 A on tight junction proteins by western blot and the three mentioned proteins, claudin, occludin-7 and ZO-1, showed a clear reduction in its expression 2 h after the dinucleotide application, the fall of expression being 59.3% for ZO-1, 57.9% for occludin-7 and 58.8% for claudin (Loma et al, 2015b).…”

Section: Effect On Epithelial Permeabilitymentioning

confidence: 99%

The role of dinucleoside polyphosphates on the ocular surface and other eye structures

Carracedo

Crooke

Guzmán-Aránguez

et al. 2016

Progress in Retinal and Eye Research

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a b s t r a c tDinucleoside polyphosphates comprises a group of dinucleotides formed by two nucleosides linked by a variable number of phosphates, abbreviated NpnN (where n represents the number of phosphates). These compounds are naturally occurring substances present in tears, aqueous humour and in the retina. As the consequence of their presence, these dinucleotides contribute to many ocular physiological processes. On the ocular surface, dinucleoside polyphosphates can stimulate tear secretion, mucin release from goblet cells and they help epithelial wound healing by accelerating cell migration rate. These dinucleotides can also stimulate the presence of proteins known to protect the ocular surface against microorganisms, such as lysozyme and lactoferrin. One of the latest discoveries is the ability of some dinucleotides to facilitate the paracellular way on the cornea, therefore allowing the delivery of compounds, such as antiglaucomatous ones, more easily within the eye. The compound Ap 4 A has been described being abnormally elevated in patient's tears suffering of dry eye, Sjogren syndrome, congenital aniridia, or after refractive surgery, suggesting this molecule as biomarker for dry eye condition. At the intraocular level, some diadenosine polyphosphates are abnormally elevated in glaucoma patients, and this can be related to the stimulation of a P2Y 2 receptor that increases the chloride efflux and water movement in the ciliary epithelium. In the retina, the dinucleotide dCp 4 U, has been proven to be useful to help in the recovery of retinal detachments. Altogether, dinucleoside polyphosphates are a group of compounds which present relevant physiological actions but which also can perform promising therapeutic benefits.

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Tight Junction Transmembrane Protein Claudin Subtype Expression and Distribution in Human Corneal and Conjunctival Epithelium

Cited by 69 publications

References 36 publications

Clinical staining of the ocular surface: Mechanisms and interpretations

Clinical staining of the ocular surface: Mechanisms and interpretations

EMMPRIN Modulates Epithelial Barrier Function through a MMP–Mediated Occludin Cleavage

The role of dinucleoside polyphosphates on the ocular surface and other eye structures

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