Thromboelastographic evaluation of hemostatic functionin dogs with dilated cardiomyopathy

Abstract

This study aimed to evaluate the hemostatic function by thromboelastography (TEG) and determine if there was an association between the changes in hemostatic parameters and serum cardiac troponin I (cTnI) in dogs with dilated cardiomyopathy (DCM). DCM was diagnosed by echocardiography in 19 dogs. Ten healthy dogs were selected as controls. Myocardial injury was confirmed by increased serum cTnI levels. Coagulation was assessed by TEG evaluating clot kinetics (reaction time [R] and kinetic time [K]), clot strengthening (alpha [α] angle and G value), platelet function (maximum amplitude [MA] and coagulation-index [CI]), clot stability (LY30, the percentage of lysis 30 min after MA), and global clotting times (activated partial thromboplastin time [aPTT] and prothrombin time [PT]). Dogs with DCM had higher R and K times but lower MA, α-angle, G, and CI compared to controls (P < 0.05). LY30 and PT did not statistically differ between groups, but the aPTT in dogs with DCM was higher than that of the controls. Serum cTnI (median [range], ng/mL) was higher in dogs with ) than in healthy dogs (0.03 [0.01-0.06]). There was no statistical relation between cTnI and coagulation parameters. This study showed that alterations of the coagulation status in dogs with DCM could develop independently of serum cTnI elevations.

Platelet proteome changes in dogs with congestive heart failure

“…Echocardiographic examinations were performed using conventional methods (2-D, M-mode, and color Doppler) and imaging techniques (right parasternal short -RPSA, and long axis -RPLA, left apical 4-5 chamber and subcostal views) with three different choices (2.5-5, 5-7.5, and 7.5-10 MHz) of phasedarray cardiac transducers in all dogs (Caris Plus Esaote, Italy) [76][77][78]. Left ventricular (LV) -related parameters such as interventricular septum (IVS) and post-wall (LVPW) thickness, LV internal dimension at diastole and systole (LVDd and LVDs), and E-point to septal separation (EPSS) were measured at RPLA 4 chamber view by 2D and M-mode echocardiography.…”

Platelet proteome changes in dogs with congestive heart failure

“…ECG was performed using 3 bipolar standard limb leads in un-sedated dogs. Cardiac rhythm analyses and measurements were performed with a standard calibration (10 mm/mV and 50 mm/sec), as reported in our previous studies (69,70,71). /body weight (kg) 0.294 (65).…”

Platelet proteome changes in dogs with congestive heart failure

“…ECG was performed using 3 bipolar standard limb leads in un-sedated dogs. Cardiac rhythm analyses and measurements were performed with a standard calibration (10 mm/mV and 50 mm/sec), as reported in our previous studies (76,77). LV diastolic function was evaluated by trans-mitral in ow pro le including early LV lling (E), late atrial contraction (A) and their ratio (E/A) by Pulsed-wave Doppler echo at apical 4-chamber view.…”

“…Echocardiographic examinations were performed using conventional methods (2-D, M-mode, and color Doppler) and imaging techniques (right parasternal short -RPSA, and long axis -RPLA, left apical 4-5 chamber and subcostal views) with three different choices (2.5-5, 5-7.5, and 7.5-10 MHz) of phased-array cardiac transducers in all dogs (Caris Plus Esaote, Italy)(76)(77)(78). Left ventricular (LV) -related parameters such as interventricular septum (IVS) and post-wall (LVPW) thickness, LV internal dimension at diastole and systole (LVDd and LVDs), and E-point to septal separation (EPSS) were measured at RPLA 4 chamber view by 2D and M-mode echocardiography.…”