Three Spectrophotometric Methods for Determination of Alogliptin Benzoate and Pioglitazone HCl in Combined Tablet Dosage Form

Mabrouk, Mokhtar M.; Hammad, Sherin F.; Mansour, Fotouh R.; Amer, Mona M.

doi:10.1080/22297928.2018.1442246

Cited by 10 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was found that the combination of ALO and PIO gives a better effect in the treatment of T2DM than the effect of single drug treatment by improving the function of beta-cells [ 10 ]. The literature review reported a few analytical techniques for the estimation of PIO and ALO including spectrophotometric methods (area under the curve, first derivative of ratio spectra, and second order derivative) [ 11 , 12 ], HPTLC [ 13 , 14 ], LC-MS/MS in human plasma [ 15 , 16 ] and RP-HPLC [ 17 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

Earth-friendly micellar UPLC technique for determination of four hypoglycemic drugs in different pharmaceutical dosage forms and spiked human plasma

et al. 2023

View full text Add to dashboard Cite

A novel, sensitive, and green micellar UPLC method was proposed and validated for the simultaneous determination of four hypoglycemic agents used in type II diabetes mellitus treatment namely, pioglitazone, alogliptin, glimepiride, and vildagliptin. The developed UPLC method was successfully applied for quantitative analysis of these drugs in bulk, in pharmaceutical formulations, and in spiked human plasma. Chromatographic separation was carried out on a Kinetex® 1.7 μm XB-C18 100 Å (50 × 2.1 mm) column, using a degassed and filtered mixture of (0.1 M SDS- 0.3% triethyl amine- 0.1% phosphoric acid (pH 6)) and n-propanol (85:15 v/v), at a flow rate of 0.2 mL/min. The experimental conditions of the suggested method were well investigated and optimized. The newly developed micellar UPLC method is capable of determining different dosage forms at the same time with the same solvents, saving time and effort. The method was found to be efficiently applicable in spiked human plasma and could be extended to study the pharmacokinetics of the cited drugs in real human plasma samples. The greenness of the developed method was evaluated by applying the Eco-scale scoring tool, which verified the excellent greenness of the analytical method.

show abstract

Section: Introductionmentioning

confidence: 99%

Earth-friendly micellar UPLC technique for determination of four hypoglycemic drugs in different pharmaceutical dosage forms and spiked human plasma

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Alogliptin benzoate is a new drug and is non official in any pharmacopoeia. In literature a number of methods for estimation of AGT in drug products, either alone or in combination with other drugs was reported using chromatographic methods [4–7], spectrophotometric methods [8], and capillary electrophoresis [9].…”

Section: Introductionmentioning

confidence: 99%

Different chromatographic methods for determination of alogliptin benzoate, metformin hydrochloride, and metformin impurity in bulk and pharmaceutical dosage form

Magdy

Ali

Taha

et al. 2021

J of Separation Science

View full text Add to dashboard Cite

Two simple, sensitive, and reproducible methods were developed for the determination of alogliptin and metformin hydrochloride in presence of metformin impurity “melamin” in pure form and in pharmaceutical formulation. Method (A) was a thin layer chromatographic method in which separation was achieved using ethyl acetate–methanol–formic acid (6:3.8:0.2, by volume) as a developing system followed by densitometric scanning at 230 nm. Method (B) was a high‐performance liquid chromatography method; separation was achieved on C18 column, the mobile phase consisted of a mixture of sodium lauryl sulfate buffer 0.1% w/v, pH 3: methanol in the ratio 70:30, v/v and measurement was done at 220 nm. System suitability testing parameters were calculated to ascertain the quality performance of the developed chromatographic methods. The proposed methods have been validated regarding accuracy, precision, and selectivity, moreover they have been successfully applied to Westirizide tablets containing both alogliptin and metformin hydrochloride, results indicate that there was no interference from additives. No significance difference was found when these methods were compared to the reported one.

show abstract

Spectrofluorometric determination of alogliptin an antidiabetic drug in pure and tablet form using fluorescamine, a fluorogenic agent: application to content uniformity test

et al. 2020

View full text Add to dashboard Cite

Alogliptin is an antidiabetic drug that belongs to a group called dipeptidyl peptidase-4 enzyme inhibitors. As the drug contains a primary amino group in its structure, it readily reacts with fluorescamine in slightly alkaline medium (borate buffer, pH 8.8) to form a highly fluorescent product. Emission of this product was measured at 477 nm (λ ex = 387 nm). The linear range between the fluorescence intensity and the drug concentration was 0.1-0.5 μg ml −1 with a good correlation coefficient (0.9986). Limits of detection and quantitation were 22 and 72 ng ml −1 , respectively. Guidelines of the International Conference for Harmonisation were followed to validate the developed method with acceptable results. Alogliptin content was determined successfully in its commercial dosage form using the fluorescamine method with good recovery (98.60-101.26%). The method has excellent levels of accuracy and precision compared with the reported method as assessed using Student's t-test and Fisher's exact test. The method was applied successfully for the content uniformity test with high recovery and low relative standard deviation.

show abstract

Three Spectrophotometric Methods for Determination of Alogliptin Benzoate and Pioglitazone HCl in Combined Tablet Dosage Form

Cited by 10 publications

References 12 publications

Earth-friendly micellar UPLC technique for determination of four hypoglycemic drugs in different pharmaceutical dosage forms and spiked human plasma

Earth-friendly micellar UPLC technique for determination of four hypoglycemic drugs in different pharmaceutical dosage forms and spiked human plasma

Different chromatographic methods for determination of alogliptin benzoate, metformin hydrochloride, and metformin impurity in bulk and pharmaceutical dosage form

Spectrofluorometric determination of alogliptin an antidiabetic drug in pure and tablet form using fluorescamine, a fluorogenic agent: application to content uniformity test

Contact Info

Product

Resources

About