Three new species of Krogia (Ramalinaceae, lichenised Ascomycota) from the Paleotropics

Abstract

Krogiaborneensis Kistenich & Timdal, K.isidiata Kistenich & Timdal and K.macrophylla Kistenich & Timdal are described as new species, the first from Borneo and the two latter from New Caledonia. The new species are supported by morphology, secondary chemistry and DNA sequence data. Krogiaborneensis and K.isidiata contain sekikaic and homosekikaic acid, both compounds reported here for the first time from the genus. Krogiamacrophylla contains an unknown compound apparently related to boninic acid as the major compound. DNA sequences (mtSSU and nrITS) are provided for the first time for Krogia and a phylogeny of the genus based on 15 accessions of five of the six accepted species is presented. Krogiaantillarum is reported as new to Brazil, Guatemala and Mexico.

A regional study of the genus Phyllopsora (Ramalinaceae) in Asia and Melanesia

“…For DNA extraction, PCR amplification and DNA sequencing of the mitochondrial ribosomal small subunit (mtSSU) and the nuclear ribosomal internal transcribed spacer region (ITS: ITS1, 5.8S, ITS2), we followed the protocols outlined in Kistenich et al (2018a). For sequence assembly and preliminary alignment, we used Geneious R9 (Kearse et al 2012).…”

“…They often differ, however, from Phyllopsora in ascospore size and arrangement, presence of prothallus, thallus construction and chemistry (Kalb and Elix 1995; Kistenich et al 2018b; Timdal 2008a; 2009). Recently, Kistenich et al (2018a) described three new species of Krogia from Asia and Oceania, which were all tentatively identified as Phyllopsora sp., indicating the morphological similarity between these two genera.…”

A revision of the genus Aciculopsora (Ramalinaceae), with the description of one new species and one new combination

“…The molecular methods for DNA extraction, PCR amplification and Sanger sequencing of the mitochondrial ribosomal small subunit (mtSSU) and of the nuclear ribosomal internal transcribed spacer region (ITS: ITS1, 5.8S, ITS2) followed those described by Kistenich et al (2018b).…”

“…Regarding the procedures employed for morphological and chemical analyses, see the Methods section in Kistenich et al (2018b).…”

“…We defined four subsets, one for mtSSU and three for ITS corresponding to the ITS1, 5.8S and ITS2 regions, and analysed those with the TESTMERGE function resembling PartitionFinder2. In addition, we analysed the dataset with MrBayes v.3.2.6 (Altekar et al 2004;Ronquist & Huelsenbeck 2003) as described in Kistenich et al (2018b). Three length parameter α/β was set to 3.4 and the temperature increment parameter to 0.15.…”