Abstract:Two new tetrahydrofuran lignans, kadlongirins A and B (1, 2), a new cadinane-type sesquiterpenoid, 2,7-dihydroxy-11,12-dehydrocalamenene (3), together with seven known lignans, grandisin, fragransin B 1 , vladirol F, kadsuralignan C, otobaphenol, isoanwulignan, and 4-[4-(3,4-dimethoxyphenyl)-2,3-dimethylbutyl]-2-methoxyphenol, were isolated from the leaves and stems of Kadsura longipedunculata. The structures of these new compounds were elucidated by spectroscopic methods. Compound 2 exhibited weak anti-human … Show more
“…The CH 2 Cl 2 extract was subjected to a silica gel column chromatography (CC) eluted with petroleum ether-acetone in a gradient (1 : 0 to 1 : 1), to afford 14 fractions (Fr. [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Fr.…”
Two new norsesquiterpenoids, oxyphyllenotriol A (1) and oxyphyllone G (2), and two new sesquiterpenoids, oxyphyllol D (3) and oxyphyllol E (4), together with two known compounds (5, 6) were isolated from the fruits of Alpinia oxyphylla. Their structures were determined on the basis of spectroscopic analysis, including high resolution electrospray ionization mass spectrometry (HR-ESI-MS), 1D-and 2D-NMR, and the absolute configurations of 2 and 4 were determined by theoretical calculation of electronic circular dichroism (ECD) and comparison with the experimental ECD spectra.
“…The CH 2 Cl 2 extract was subjected to a silica gel column chromatography (CC) eluted with petroleum ether-acetone in a gradient (1 : 0 to 1 : 1), to afford 14 fractions (Fr. [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Fr.…”
Two new norsesquiterpenoids, oxyphyllenotriol A (1) and oxyphyllone G (2), and two new sesquiterpenoids, oxyphyllol D (3) and oxyphyllol E (4), together with two known compounds (5, 6) were isolated from the fruits of Alpinia oxyphylla. Their structures were determined on the basis of spectroscopic analysis, including high resolution electrospray ionization mass spectrometry (HR-ESI-MS), 1D-and 2D-NMR, and the absolute configurations of 2 and 4 were determined by theoretical calculation of electronic circular dichroism (ECD) and comparison with the experimental ECD spectra.
“…Kadsura longipedunculata Finet et Gagnep (Nanwuweizi), which is a type of traditional Chinese medicine, is widely distributed in the southern part of China [3,4]. Kadsura longipedunculata (KL) has been used for the treatment of a variety of diseases, including tumors, hepatitis [5,6], inflammation [7], immunosuppression [8], and insomnia [9,10], for thousands of years.…”
Objectives
Kadsura longipedunculata (KL) has been widely used for the treatment of insomnia in traditional Chinese medicine. The aim of this study was to explore the mechanism of the sedative and hypnotic effects of KL.Materials and MethodsThe content of KL was evaluated by HPLC-TOF-MS, and a potential target was found and used to construct its 3D structure to screen for potential ligands among the compounds in KL by using bioinformatics analysis, including similarity ensemble approach (SEA) docking, homology modeling, molecular docking and ligand-based pharmacophore. The PCPA-induced insomnia rat model was then applied to confirm the potential targets related to the sedative effects of KL by performing the forced swimming test (FST), the tail suspension test (TST) and the measurement of target-related proteins using western blotting and immunofluorescence.ResultsBioinformatics analysis showed that most of lignan compounds in KL were optimal ligands for the 5-HT1A receptor (5-HT1AR), and they were found to be potential targets related to sedative effects; the main lignan content of KL extracts was characterized by HPLC-TOF-MS, with 7 proposed lignans detected. Administration of KL could significantly reduce FST and TST immobility time in the PCPA-induced 5HT-depleted insomnia rat model. The expressions of proteins related to the 5-HT1AR pathway were regulated by extracts of KL in a concentration-dependent manner, indicating that extracts of KL had 5-HT1AR agonist-like effects.Conclusion
In silico analysis and experimental validation together demonstrated that lignan extracts from KL can target 5-HT1AR in insomniac rats, which could shed light on its use as a potential 5-HT1AR agonist drug.
Schisanpropinoic acid (1), a new bergamotane sesquiterpenoid, and schisanpropinin (2), a new tetrahydrofuran lignan with a rare epoxyethane unit, were identified from the stems and leaves of Schisandra propinqua var. propinqua. Their structures were determined based on comprehensive spectroscopic and mass spectrometric analysis. The absolute configuration of 1 was determined by X-ray analysis. Compounds 1 and 2 were tested for their cytotoxic activity against five human tumor cell lines.Graphical Abstract
Electronic supplementary materialThe online version of this article (doi:10.1007/s13659-017-0129-7) contains supplementary material, which is available to authorized users.
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