Three-Dimensional Structure and Biophysical Characterization of Staphylococcus aureus Cell Surface Antigen–Manganese Transporter MntC

Transcriptional Regulation, Metal Binding Properties and Structure of Pden1597, an Unusual Zinc Transport Protein from Paracoccus denitrificans

“…To our knowledge, the structures of at least 10 different cluster 9 SBPs from various organisms have been determined by x-ray crystallography. Based on the above criteria, only the ZnuA homologues (14, 16 -19) are predicted to be specific for Zn 2ϩ , whereas the SitA (15), MntC (21,22,25), PsaA (20,23,24), and MtsA (26) homologues should be specific for Mn 2ϩ . TroA is the only member of the group that violates these rules, having both His-205 (Zn-specific) and Asp-289 (Mn-specific) ligands and no His-rich loop (Mn-specific) (27)(28)(29) for 4-and 6-coordinate environments, respectively (53).…”

Lipoproteins of Gram-Positive Bacteria: Key Players in the Immune Response and Virulence

“…Indeed, it has been shown that active immunization with MntC reduced the bacterial load of S. aureus and S. epidermidis infection in an acute murine bacteremia model, and antiMntC monoclonal antibodies were protective in a rat passive immunization model and induced neutrophil respiratory burst activity (122). Moreover, MntC from S. aureus and SitA from S. pseudintermedius are orthologous, show high sequence identity, and have been well characterized biochemically and structurally, suggesting that they may be suitable for industrial-scale production as staphylococcal vaccine antigens (1,123).…”

Manganese uptake and streptococcal virulence

“…The sequences of the 38 SBPs included in this study were obtained from the NCBI and had the following GI numbers: AdcA_Spn, GI:116516060 (Bayle et al 2011); AdcAII_Spn, GI:116515739 (Bayle et al 2011; Loisel et al 2008); FimA_Spa, GI:504525723 (Oetjen et al 2002); Lbp_Spy, GI:16151821 (Linke et al 2008; Linke et al 2009); Lmb_Sag, GI:22537388 (Ragunathan et al 2009a; Ragunathan et al 2009b); MntA_Ban, GI:229264967 (Gat et al 2005); MtsA_Sag, GI:22537676 (Bray et al 2009); MtsA_Spy, GI:383493478 (Sun et al 2009; Sun et al 2008); MntC_Ngo, GI:59800624 (Lim et al 2008); MntC_Sau, GI:88194402 (Horsburgh et al 2002a); MntC_Syn, GI:1653002 (Adir et al 2002); MtsA_Sin, GI:343481042 (Zou et al 2010); MtuA_Sub, GI:222113460 (Banerjee et al 2003); PsaA_Spn, GI:116515973 (McDevitt et al 2011); ScaA_Sgo, GI:310633 (Jakubovics et al 2000; Kolenbrander et al 1998); SitA_Eco, GI:84060846 (Sabri et al 2006); SitA_Sen, GI:16761638 (Zhou et al 1999); SitA_Sme, GI:15966779 (Chao et al 2004; Davies and Walker 2007; Platero et al 2004); SitA_Sps, GI:10135086 (Abate et al 2014); SitC_Sep, GI:57636840 (Cockayne et al 1998; Massonet et al 2006); SloC_Smu, GI:8925939 (Paik et al 2003); SsaB_San, GI:153826 (Crump et al 2014); TroA_Ssu, GI:386583456 (Wichgers Schreur et al 2011; Zheng et al 2011); TroA_Tpa, GI:1777933 (Deka et al 1999; Desrosiers et al 2007; Lee et al 1999; Lee et al 2002); YfeA_Ype, GI:1245464 (Bearden and Perry 1999; Bearden et al 1998); ZnuA_Cje, GI:384442496 (Davis et al 2009); ZnuA_Eco, GI:298380994 (Patzer and Hantke 1998); ZnuA_Hin, GI:16272089 (Lu et al 1997); ZnuA_Sen, GI:161353595 (Ammendola et al 2007; Ilari et al 2011); ZnuA_Syn, GI:38492833 (Banerjee et al 2003); ZnuA_Tpa, GI:378974662 (Desrosiers et al 2007); ZnuA_Vpa, GI:28901162 (Gribenko et al 2013); ZnuA_Ype, GI:22126135 (Bobrov et al 2014); ZnuA_Yru, GI:238754865 (Dahiya and Stevenson 2010); YcdH_Bsu, GI:2415736 (Gaballa and Helmann 1998; Gaballa et al 2002). …”