Three‐dimensional reconstruction of deferent ducts papillae in urogenital duct system of the male rat

Wakui, Shin; Motohashi, Masaya; Inomata, Tomo; Ichihara, Nobutsune; Mutou, Tomoko; Takahashi, Hiroyuki; Ikegami, Machiko; Asari, Masao

doi:10.1002/pros.22948

Cited by 3 publications

(5 citation statements)

References 11 publications

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“…Prior to commencement, the suitability of the mouse urogenital tract as a model for human prostatic NE cells had to be verified. As had been shown previously for the rat urogenital tract ( 6 ), the distribution pattern of murine prostatic NE cells differs from the situation in humans in that, in the former, the NE cells are generally absent from the prostatic glandular epithelium proper and restricted to the terminal gland ducts of the ventral (and less of the lateral and dorsal) prostate and the urethral opening of the terminal excretory duct of the seminal vesicle (even though the definite topographical situation is controversial ( 27 )).…”

Section: Discussionmentioning

confidence: 91%

Neuroendocrine Cells of the Prostate Derive from the Neural Crest

Szczyrba

Niesen

Wagner

et al. 2017

Journal of Biological Chemistry

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The histogenesis of prostatic neuroendocrine cells is controversial: a stem cell hypothesis with a urogenital sinus-derived progeny of all prostatic epithelial cells is opposed by a dual origin hypothesis, favoring the derivation of neuroendocrine cells from the neural crest, with the secretory and basal cells being of urogenital sinus origin. A computer-assisted 3D reconstruction was used to analyze the distribution of chromogranin A immunoreactive cells in serial sections of human fetal prostate specimens (gestation weeks 18 and 25). Immunohistochemical double labeling studies with YFP and serotonin antisera combined with electron microscopy were carried out on double-transgenic Wnt1-Cre/ROSA26-YFP mice showing stable YFP expression in all neural crest-derived cell populations despite loss of Wnt1 expression. 3D reconstruction of the distribution pattern of neuroendocrine cells in the human fetal prostate indicates a migration of paraganglionic cells passing the stroma and reaching the prostate ducts. Double-transgenic mice showed 55% double labeling of periurethral neuroendocrine cells expressing both serotonin and YFP, whereas single serotonin labeling was observed in 36% and exclusive YFP labeling in 9%. The results favor the assumption of a major fraction of neural crest-derived neuroendocrine cells in both the human and murine prostates.

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Section: Discussionmentioning

confidence: 91%

Neuroendocrine Cells of the Prostate Derive from the Neural Crest

Szczyrba

Niesen

Wagner

et al. 2017

Journal of Biological Chemistry

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“…Variations in the number and arrangement of the ducts draining each male accessory sex glands have been observed in several animal species (Price, ; Jones et al., ; Schehata, ; Oh et al., ; Aumuller et al., ). In rats, the seminal vesicle ducts are located dorsally to the ampullary glands (Wakui et al., ), and an ejaculatory sinus pair is formed by the fusion of these two ducts (Jones et al., ; Aumuller et al., ). In rats, the deferent ducts drain into the main ducts of ampullary glands with lack of the ejaculatory ampullae (Wakui et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…In rats, the seminal vesicle ducts are located dorsally to the ampullary glands (Wakui et al., ), and an ejaculatory sinus pair is formed by the fusion of these two ducts (Jones et al., ; Aumuller et al., ). In rats, the deferent ducts drain into the main ducts of ampullary glands with lack of the ejaculatory ampullae (Wakui et al., ). Although the ejaculatory ducts system have been described to be composed of the deferent ducts that receive the seminal vesicle ducts by recent macroscopical and microscopical observations (Aumuller et al., ; : Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In the male accessory sex glands of rats, the prostate and coagulation glands are considered to be the origin of the urogenital sinus, while the ejaculatory duct system, including the deferent ducts, seminal vesicles, ampullary glands, and the ducts of all prostate lobes, is considered to derive from the Wolffian ducts (Price, ; Jones et al., ; Jesik et al., ; Tamano et al., ). While because of its anatomical complexity, a uniform histopathological evaluation of the accessory sex glands is difficult and, therefore, may introduce observer bias (Aumuller et al., ; Wakui et al., ). Although the ejaculatory duct system of male rodents has been previously described, major concerns remain regarding the formation of the terminal regions of ejaculatory duct system (Tamano et al., ; Pinheiro et al., ; Aumuller et al., ; Wakui et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…While because of its anatomical complexity, a uniform histopathological evaluation of the accessory sex glands is difficult and, therefore, may introduce observer bias (Aumuller et al., ; Wakui et al., ). Although the ejaculatory duct system of male rodents has been previously described, major concerns remain regarding the formation of the terminal regions of ejaculatory duct system (Tamano et al., ; Pinheiro et al., ; Aumuller et al., ; Wakui et al., ). The purpose of this study was to describe, in detail, the structure of the terminal portion of the ejaculatory ducts in rat using three‐dimensional reconstruction methods with serial sections to provide data for further anatomical and/or experimental studies of ejaculatory system on rats.…”

Section: Introductionmentioning

confidence: 99%

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Three-Dimensional Study of the Terminal Portion in Sprague-Dawley Rat Ejaculatory Ducts

Motohashi

Inomata

Takahashi

et al. 2015

Anat. Histol. Embryol.

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In mammals, a pair of ejaculatory ducts exists in the urethra at the seminal colliculus. The detailed anatomical structures of the distal end of the ejaculatory ducts of Sprague-Dawley rats were investigated by the computer-assisted three-dimensional reconstruction analysis using light-microscopic serial sections. A three-dimensional reconstruction revealed that in adult rats, the ejaculatory sinus pair consists of two parts: the cranial section - a compartment region composed of a fusion of the ampullary gland duct and the seminal vesicle duct, and the caudal section - a grooved region composed of a long slitlike ejaculatory ostium that extends into the urethra on both sides of the seminal colliculus. But the sphincter structure was not observed. The long axis of the compartment region was approximately 58 μm in length, and that of the groove region was approximately 495 μm. Although many epithelial glands ducts were distributed throughout the ejaculatory sinuses, the prostate and coagulation gland ducts did not open in these sinuses. The urethra was composed of transitional epithelium, while the ejaculatory sinuses were composed of single to stratified cuboidal epithelium. The ejaculatory ducts continued to the ejaculatory ostium in male adult Sprague-Dawley rat were composed of the seminal vesicle ducts received the ampullary gland ducts.

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Study on multi-density contrast agent fillers of duct casting based on CT three-dimensional reconstruction

Huang

Chen²,

Wang

et al. 2017

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The three-dimensional visualization model of human body duct is based on virtual anatomical structure reconstruction with duct angiography, which realizes virtual model transferred from two-dimensional, planar and static images into three-dimensional, stereoscopic and dynamic ones repectively. In recent years, the multi-duct segmentation and division of the same specimen (or organ) is the focus of attention shared by surgeons and clinical anatomists. On the basis of 4.22 g/cm body bone density, this study has screened out metal oxide contract agent with different density for infusion and modeling, as well as compared and analyzed the effects of three-dimensional image of CT virtual bronchoscopy (CTVB), three-dimensional image of CT maximum intensity projection and three-dimensional model. This experiment result showed synchronously infusing multi-duct of same specimen (or organ) with contrast agent in different densities could reconstruct three-dimensional models of all ducts once only and adjust threshold to develop single or multiple ducts. It was easier to segment and observe the duct structure, anastomosis, directions and crossing in different parts, which was beyond comparison with three-dimensional image of CTVB. Although the existing three-dimensional duct reconstruction techniques still cannot be applied in living bodies temporarily, this study focused on a creative design of ducts segmentation in different density, which proposed a new experimental idea for developing multi-duct three-dimensional model in living body in the future. It will play a significant role in disease diagnosis and individual design in surgical treatment program. Therefore, this study observes the three-dimensional status of human duct with the application of contrast agent fillers in different density, combined with three-dimensional reconstruction technology. It provides an innovative idea and method for constructing three-dimensional model of digital multi-duct specimen, and the ultimate goal is to develop the digitized virtual human and precise medical treatment better and faster.

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Three‐dimensional reconstruction of deferent ducts papillae in urogenital duct system of the male rat

Abstract: The deferent ducts of rat terminated at the deferent ducts papillae that located at the main duct of ampullary glands that drained into the urethra. The deferent ducts papillae might be controlled by the expansion/contraction of well-developed papillary mucosal capillary vessels.

Cited by 3 publications

References 11 publications

Neuroendocrine Cells of the Prostate Derive from the Neural Crest

Neuroendocrine Cells of the Prostate Derive from the Neural Crest

Three-Dimensional Study of the Terminal Portion in Sprague-Dawley Rat Ejaculatory Ducts

Study on multi-density contrast agent fillers of duct casting based on CT three-dimensional reconstruction

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