Three-Dimensional Reconstruction of Brain-wide Wiring Networks in Drosophila at Single-Cell Resolution

Chiang, Ann‐Shyn; Lin, Chih Yung; Chuang, Chao Chun; Chang, Hsiu Ming; Hsieh, Chang Huain; Yeh, Chang Wei; Shih, C. T.; Wu, Jian Jheng; Wang, Guo Tzau; Chen, Yung Chang; Wu, Cheng Chi; Chen, Guan Yu; Ching, Yu-Tai; Lee, Ping Chang; Lin, Chen‐Yuan; Lin, Hui Hao; Wu, Chia‐Chou; Hsu, Hui-Yu; Huang, Yun Ann; Chen, Jing Yi; Chiang, Hsin Jung; Lu, Chun; Ni, Ru Fen; Yeh, Chao‐Yuan; Hwang, Jenn Kang

doi:10.1016/j.cub.2010.11.056

Cited by 626 publications

(648 citation statements)

References 52 publications

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“…On the day after eclosion, D. melanogaster males were assigned randomly as rival or focal males. Males from other species were used as rival males and given an identifying wing clip using light CO 2 anesthesia, a procedure that does not affect the response of D. melanogaster focal males to rivals (Bretman, Westmancoat et al., 2011). Focal males were then held on their own or exposed to a rival for 3 days.…”

Section: Methodsmentioning

confidence: 99%

“…In order to inform our experimental design, we first assessed whether conflicting data on the role of visual cues in responses to conspecific rivals within D. melanogaster (Bretman, Westmancoat et al., 2011; Kim et al., 2012) could arise from strain differences. To do this, we replicated the same design as Kim et al.…”

Section: Methodsmentioning

confidence: 99%

“…Territorial competitors tend to be recognized through the detection of multiple cues within the same, or different, sensory modalities (Grether, 2011), and multimodal cues can also be used in the evaluation of sperm competition threat. For example, in D. melanogaster , any paired combination of sound, smell, and touch is required in order for males to respond to a conspecific rival (Bretman, Westmancoat, Gage, & Chapman, 2011), and manipulations of single sensory cues slow the speed with which D. melanogaster males can swap between high and low sperm competition modes (Rouse & Bretman, 2016). Conflicting data suggest that male D. melanogaster require only visual cues, specifically the perception of the red eyes of another fly, in order to respond to D. simulans or D. virilis males as rivals (Kim, Jan, & Jan, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…In order to confirm that we were manipulating the important sensory modalities, we first examined whether strain differences could explain conflicting reports on the role of visual cues in rivals’ responses within D. melanogaster (Bretman, Westmancoat et al., 2011; Kim et al., 2012). We then tested whether D. melanogaster males responded to males of four other species when exposed to a full sensory repertoire from the heterospecifics or when single sensory cues were removed in turn (Bretman, Westmancoat et al., 2011).…”

Section: Introductionmentioning

confidence: 99%

“…We then tested whether D. melanogaster males responded to males of four other species when exposed to a full sensory repertoire from the heterospecifics or when single sensory cues were removed in turn (Bretman, Westmancoat et al., 2011). This enabled us to test two predictions: (1) Given a full sensory repertoire, males should avoid investing in “rivals’ responses” to heterospecifics that pose no sperm competition threat and (2) the sensory modalities used to convey species‐specific information can be identified by manipulating cues in order to “trick” males into responding to heterospecific rivals as they would to conspecifics.…”

Section: Introductionmentioning

confidence: 99%

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The role of species‐specific sensory cues in male responses to mating rivals in Drosophila melanogaster fruitflies

Bretman

Rouse

Westmancoat

et al. 2017

Ecology and Evolution

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Complex sets of cues can be important in recognizing and responding to conspecific mating competitors and avoiding potentially costly heterospecific competitive interactions. Within Drosophila melanogaster, males can detect sensory inputs from conspecifics to assess the level of competition. They respond to rivals by significantly extending mating duration and gain significant fitness benefits from doing so. Here, we tested the idea that the multiple sensory cues used by D. melanogaster males to detect conspecifics also function to minimize “off‐target” responses to heterospecific males that they might encounter (Drosophila simulans, Drosophila yakuba, Drosophila pseudoobscura, or Drosophila virilis). Focal D. melanogaster males exposed to D. simulans or D. pseudoobscura subsequently increased mating duration, but to a lesser extent than following exposure to conspecific rivals. The magnitude of rivals’ responses expressed by D. melanogaster males did not align with genetic distance between species, and none of the sensory manipulations caused D. melanogaster to respond to males of all other species tested. However, when we removed or provided “false” sensory cues, D. melanogaster males became more likely to show increased mating duration responses to heterospecific males. We suggest that benefits of avoiding inaccurate assessment of the competitive environment may shape the evolution of recognition cues.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The role of species‐specific sensory cues in male responses to mating rivals in Drosophila melanogaster fruitflies

Bretman

Rouse

Westmancoat

et al. 2017

Ecology and Evolution

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Programmed cell death acts at different stages of Drosophila neurodevelopment to shape the central nervous system

2016

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Edited by Wilhelm JustNervous system development is a process that integrates cell proliferation, differentiation, and programmed cell death (PCD). PCD is an evolutionary conserved mechanism and a fundamental developmental process by which the final cell number in a nervous system is established. In vertebrates and invertebrates, PCD can be determined intrinsically by cell lineage and age, as well as extrinsically by nutritional, metabolic, and hormonal states. Drosophila has been an instrumental model for understanding how this mechanism is regulated. We review the role of PCD in Drosophila central nervous system development from neural progenitors to neurons, its molecular mechanism and function, how it is regulated and implemented, and how it ultimately shapes the fly central nervous system from the embryo to the adult. Finally, we discuss ideas that emerged while integrating this information.Keywords: apoptosis; Drosophila; neurodevelopment Apoptosis shapes Drosophila neural development from the emergence of neuroectoderm in the embryo to the eclosing adult. The earliest indication of programmed cell death (PCD) during neurodevelopment is at embryonic stages 11-12 [1-3], when the first neurons and epidermal cells die [4,5]. Neuronal apoptosis then increases dramatically peaking at embryonic stages 16-17, when the ventral nerve cord (VNC) condenses [3] and the embryo produces its first twitching movements [1]. Although the observed amount of neuronal death differs from embryo to embryo [5], symmetry in neuronal PCD is often observed between the right and left sides of a single embryo [1], indicating that both deterministic and 'random' processes participate in the selection of surviving neurons.Programmed cell death in Drosophila neural development comes in different flavors. It can be triggered by spatial, temporal, nutritional, hormonal, and metabolic signals; it can be regulated by Hox genes, Notch, and other signaling pathways; it can be mediated by one or more proapoptotic genes; and it can act at the stage of neural precursors, of newly born or of mature neurons/glia. What is clear is that dying is not trivial for a cell. Many layers of regulation exist that ensure

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Immunohistochemical techniques

Shiosaka¹,

Tohyama²

2014

Insect Histology

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Three-Dimensional Reconstruction of Brain-wide Wiring Networks in Drosophila at Single-Cell Resolution

Abstract: We found that the Drosophila brain is assembled from families of multiple LPUs and their interconnections. This provides an essential first step in the analysis of information processing within and between neurons in a complete brain.

Cited by 626 publications

References 52 publications

The role of species‐specific sensory cues in male responses to mating rivals in Drosophila melanogaster fruitflies

The role of species‐specific sensory cues in male responses to mating rivals in Drosophila melanogaster fruitflies

Programmed cell death acts at different stages of Drosophila neurodevelopment to shape the central nervous system

Immunohistochemical techniques

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