Three-dimensional culture conditions lead to decreased radiation induced cytotoxicity in human mammary epithelial cells

Sowa, Marianne B.; Chrisler, William B.; Zens, Kyra D.; Ashjian, Emily; Opresko, Lee K.

doi:10.1016/j.mrfmmm.2010.03.004

Cited by 32 publications

(26 citation statements)

References 30 publications

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“…This observation was confirmed in 2008 by Fourre et al, demonstrating the protective effect of the extracellular matrix on the HT1080 cell line, against the action of doxorobucin 10 . Reduced radiation induced toxicity when cells are grown in a 3D environment has also been recently demonstrated 11 . Numerous other publications can be found in the literature describing modification of the cell phenotype, metabolism or composition when grown in a 3D matrix, compared to 2-D environments 12,13 .…”

Section: Introductionmentioning

confidence: 85%

Imaging live cells grown on a three dimensional collagen matrix using Raman microspectroscopy

Bonnier¹,

Knief²,

Lim³

et al. 2010

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Section: Introductionmentioning

confidence: 85%

Imaging live cells grown on a three dimensional collagen matrix using Raman microspectroscopy

Bonnier¹,

Knief²,

Lim³

et al. 2010

Analyst

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“…The impact of such matrices on the cell phenotype and metabolism has been already documented in the literature. For example, it has been shown that antibodies against B1-integrins exhibit different behaviour when tested in 2D compared to 3D models (Wang et al, 1998); induced doxorubicin-resistance by the extra cellular matrix in human osteosarcoma and HT1080 cells has been demonstrated (Fourre et al, 2008;Harisi et al, 2007); reduced radiation induced toxicity when cells are grown in a 3D environment has also been reported (Sowa et al, 2010). The use of collagen matrices as 3-D cell culture matrices has greatly increased in the last few years and numerous other publications can be found in the literature describing modification of the cell phenotype, metabolism or composition when grown in a 3D matrix, compared to 2-D environments (Lupanova et al, 2010;Wu et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models

Bonnier¹,

Keating²,

Wróbel

et al. 2015

Toxicology in Vitro

203

164

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“…The use of collagen gels or other commercially developed systems are reported to provide the cells with a microenvironment or matrix which is analogous to the extra cellular matrix (ECM) experienced by cells in vivo and is widely considered to be a better representation of the cancerous cell phenotype when compared to those grown in conventional 2D culture (Petersen et al, 1992, Prestwich, 2008, Weaver et al, 1995. The effects on cells grown in such systems compared to 2D systems is widely documented and it has been reported that cells can exhibit different behaviour when cultured in 3D when compared to 2D, such as toxicological response, phenotype, metabolism and composition (Sowa et al;2010;Wang et al, 1998;Fourre et al, 2008;Harisi et al) .…”

Section: Introductionmentioning

confidence: 99%

“…For example, it has been shown that antibodies against B1-integrins exhibit different behaviour when tested in 2D compared to 3D models (Wang et al, 1998); induced doxorubicin-resistance by the extra cellular matrix in human osteosarcoma and HT1080 cells has been demonstrated (Fourre et al, 2008;Harisi et al, 2007); and reduced radiation induced toxicity when cells are grown in a 3D environment has also been reported (Sowa et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Chemotherapeutic efficiency of drugs in vitro: Comparison of doxorubicin exposure in 3D and 2D culture matrices

Casey¹,

Gargotti²,

Bonnier

et al. 2016

Toxicology in Vitro

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The interest in the use of 3D matrices for in vitro analysis, with a view to increasing the relevance of in vitro studies and reducing the dependence on in vivo studies, has been growing in recent years.Cells grown in a 3D in vitro matrix environment have been reported to exhibit significantly different properties to those in a conventional 2D culture environment. However, comparison of 2D and 3D cell culture models have recently been noted to result in differing responses of cytotoxic assays, without any associated change in viability. The effect was attributed to differing conversion rates and effective concentrations of the resazurin assay in 2D and 3D environments, rather than differences in cellular metabolism. In this study, the efficacy of a chemotherapeutic agent, doxorubicin, is monitored and compared in conventional 2D and 3D collagen gel exposures of immortalized human cervical cells. Viability was monitored with the aid of the Alamar Blue assay and drug internalisation was verified using confocal microscopy. Drug uptake and retention within the collagen matrix was monitored by absorption spectroscopy. The viability studies showed apparent differences between the 2D and 3D culture systems, the differences attributed in part to the physical transition from 2D to a 3D environment causing alterations to dye resazurin uptake and conversion rates. The use of 3D culture matrices has widely been interpreted to result in "reduced" toxicity or cellular "resistance" to the chemotherapeutic agent. The results of this study show that the reduced efficiency of the drug to cells grown in the 3D environment can be accounted for by a sequential reduction of the effective concentration of the test compound and assay. This is due to absorption within the collagen gel inducing a higher uptake of both drug and assay thereby influencing the toxic impact of the drug and conversion rate of resazurin, and. the increased effective surface area of the cell exposed to the drug and assay in the 3D environment. The effect was noted to be higher after shorter exposure periods and should be accounted for in in vitro 2D and 3D culture environment comparisons.

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Three-dimensional culture conditions lead to decreased radiation induced cytotoxicity in human mammary epithelial cells

Cited by 32 publications

References 30 publications

Imaging live cells grown on a three dimensional collagen matrix using Raman microspectroscopy

Imaging live cells grown on a three dimensional collagen matrix using Raman microspectroscopy

Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models

Chemotherapeutic efficiency of drugs in vitro: Comparison of doxorubicin exposure in 3D and 2D culture matrices

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