Three-Dimensional Chiral Imaging by Sum-Frequency Generation

Ji, Na; Zhang, Kai; Yang, Haw; Shen, Y. R.

doi:10.1021/ja057775y

Cited by 61 publications

(64 citation statements)

References 18 publications

(33 reference statements)

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“…SFG measurements on aligned type I collagen have revealed that a symmetric methylene mode constitutes a major component of the achiral optical response, whereas carbonyl amide I vibrational modes are identified as chiral contributions in the SFG spectrum (50). Although these measurements have been performed only on macroscopic samples, similar experiments can be conducted in an optical microscope, providing access to detailed structural chemical information on a microscopic scale (44, 51). …”

Section: Vibrational Contrastmentioning

confidence: 99%

Biomolecular Imaging with Coherent Nonlinear Vibrational Microscopy

Chung

Potma

2013

Annu. Rev. Phys. Chem.

105

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Optical imaging with spectroscopic vibrational contrast is a label-free solution for visualizing, identifying, and quantifying a wide range of biomolecular compounds in biological materials. Both linear and nonlinear vibrational microscopy techniques derive their imaging contrast from infrared active or Raman allowed molecular transitions, which provide a rich palette for interrogating chemical and structural details of the sample. Yet nonlinear optical methods, which include both second-order sum-frequency generation (SFG) and third-order coherent Raman scattering (CRS) techniques, offer several improved imaging capabilities over their linear precursors. Nonlinear vibrational microscopy features unprecedented vibrational imaging speeds, provides strategies for higher spatial resolution, and gives access to additional molecular parameters. These advances have turned vibrational microscopy into a premier tool for chemically dissecting live cells and tissues. This review discusses the molecular contrast of SFG and CRS microscopy and highlights several of the advanced imaging capabilities that have impacted biological and biomedical research.

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Section: Vibrational Contrastmentioning

confidence: 99%

Biomolecular Imaging with Coherent Nonlinear Vibrational Microscopy

Chung

Potma

2013

Annu. Rev. Phys. Chem.

105

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“…SFG is selectively sensitive to non-centrosymmetric structures. Ji et al constructed the first optically active SFG microscope, which allows chirality-sensitive, 3-D sectional images with submicron spatial resolution [35]. SHG is a special case of SFG in which the two excitation photons are degenerate.…”

Section: Overview Of Nlo Modalitiesmentioning

confidence: 99%

Multimodal nonlinear optical microscopy

Yue

Slipchenko

Cheng

2011

Laser & Photonics Reviews

147

114

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Because each nonlinear optical (NLO) imaging modality is sensitive to specific molecules or structures, multimodal NLO imaging capitalizes the potential of NLO microscopy for studies of complex biological tissues. The coupling of multiphoton fluorescence, second harmonic generation, and coherent anti-Stokes Raman scattering (CARS) has allowed investigation of a broad range of biological questions concerning lipid metabolism, cancer development, cardiovascular disease, and skin biology. Moreover, recent research shows the great potential of using CARS microscope as a platform to develop more advanced NLO modalities such as electronic-resonance-enhanced four-wave mixing, stimulated Raman scattering, and pump-probe microscopy. This article reviews the various approaches developed for realization of multimodal NLO imaging as well as developments of new NLO modalities on a CARS microscope. Applications to various aspects of biological and biomedical research are discussed.

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“…Subsequently, thin layers of enantiomeric BN solutions containing micrometer sized silica beads have been used to prove that chiral imaging is in principle possible from bulk samples by scanning for SFG intensities [7]. More recently, the authors of the present work have tried to probe the potential of this latter technique for solid samples using the same BN molecule trapped in a sol-gel matrix [8].…”

Section: Introductionmentioning

confidence: 96%