Thioredoxin upregulation by 5‐aminolaevulinic acid‐based photodynamic therapy in human skin squamous cell carcinoma cell line

Kuhara, Tomoe; Watanabe, Daisuke; Akita, Yoichi; Takeo, Tomohiro; Ishida, Natsuko; Nakano, Aki; Yamashita, Noriko; Ohshima, Yuichiro; Kawada, Morihiro; Yanagishita, Takeshi; Tamada, Yasuhiko; Matsumoto, Yoshinari

doi:10.1111/j.1600-0781.2008.00354.x

Cited by 7 publications

(5 citation statements)

References 31 publications

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“…Depletion of glutathione with buthionine sulfoximine significantly potentiates the antitumor effects of PDT both in vitro and in vivo (45). Low-dose ALA-PDT was shown to induce thioredoxin (TRX) expression that was associated with tumor cell death prevention (47). Moreover, methylene blue-mediated PDT altered the expression of peroxiredoxins (PRXs) (48), however the exact role of this enzyme family in PDT effectiveness remains to be elucidated.…”

Section: Ros-scavenging Enzymesmentioning

confidence: 99%

Approaches to improve photodynamic therapy of cancer

Firczuk

Winiarska²,

Szokalska³

et al. 2011

Front Biosci

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Photodynamic therapy (PDT) is a clinically approved method of tumor treatment. Its unique mechanism of action results from minimal invasiveness and high selectivity towards transformed cells. However, visible light used to excite most photosensitizers has rather limited ability to penetrate tissues resulting in insufficient destruction of deeply seated malignant cells. Therefore, novel strategies for further potentiation of the anticancer effectiveness of PDT have been developed. These include combined treatments with surgery, chemo- and radiotherapy, strategies targeting cytoprotective mechanisms induced in PDT-treated cells, as well as attempts aimed at enhancement of PDT-mediated antitumor immune response. Moreover, new photosensitizers and novel light sources are being developed. Impressive progress in nanotechnology and understanding of tumor cell biology rise hopes for further improvements in this elegant and promising method of cancer treatment.

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Section: Ros-scavenging Enzymesmentioning

confidence: 99%

Approaches to improve photodynamic therapy of cancer

Firczuk

Winiarska²,

Szokalska³

et al. 2011

Front Biosci

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“…Therefore, TrxR plays important roles in controlling the reduced intracellular redox environment, cellular growth, defense against oxidative stress, or control of apoptosis . Moreover, in PDT‐treated cells both general TrxR protein level and its enzymatic activity were induced in response to an increased intracellular ROS level . Based on these facts, we hypothesized that suppression of TrxR can enhance intracellular ROS level and the anticancer effect induced by PDT.…”

Section: Introductionmentioning

confidence: 98%

Au Nanoclusters and Photosensitizer Dual Loaded Spatiotemporal Controllable Liposomal Nanocomposites Enhance Tumor Photodynamic Therapy Effect by Inhibiting Thioredoxin Reductase

Gao

Zheng

Gao

et al. 2017

Adv Healthcare Materials

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Photodynamic therapy (PDT) is a minimally invasive therapeutic procedure of tumors with high selectivity and low side effect. However, it is usually not efficient in long-lasting tumor control. One of the main reasons is tumor cells develop some protective mechanisms that help them to deal with oxidative stress in the environment. The thioredoxin system in cancer is an important antioxidant defense system. Au nanoclusters could effectively inhibit thioredoxin reductase (TrxR) in tumor cell cytoplasm. Herein, Au nanoclusters and photosensitizer Chlorine 6 (Ce6) are co-loaded in spatiotemporal controllable liposomal nanocomposites. pH responsive molecule inserted in lipid bilayer greatly contributes to the instability of the lipid membrane in lysosomal at low pH environment. Then the payloads can rapidly release into cytoplasm. Au nanoclusters effectively inhibit TrxR in cytoplasm and enhance the photodynamic-induced intracellular reactive oxygen-free radical concentration, improving the effect of PDT. Breast cancer is chosen as a tumor model and the Au nanoclusters and photosensitizer co-loaded liposomal nanocomposites are studied to improve the effect of PDT both in vitro and in vivo, and its corresponding mechanism is investigated. This study develops a new application of gold nanoclusters and provides a new train of thoughts for enhancing the effect of PDT.

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“…In contrast , high-dose exposure of cells to ALA-PDT, caspase-3 expression, and cell death due to beneficial apoptosis and/or necrosis were observed whereas, Trx was barely detected, thereby contributing to the, in this case, beneficial apoptosis. Based on these results, the authors stated that Trx, as a common antioxidant, suppressed apoptosis and facilitated cell growth (not beneficial in the case of cancer cells)(Kuhara et al 2008). Beneficial apoptosis appeared to occur with the high-dose exposure of cells to the ALA-PDT when the Trx was barely detected.…”

mentioning

confidence: 93%

“…In superficial skin cancers, the 5-aminolevulinic acid-based photodynamic 13 therapy (ALA-PDT) is widely used in clinical settings. This results in favorable outcomes, although residual tumor and tumor recurrence occasionally occur.Kuhara et al investigated Trx expression following ALA-PDT in a human skin squamous cell carcinoma cell line, HSC-Kuhara et al 2008). They found that Trx expression was only observed following a low-dose exposure of the ALA-PDT and that multiple low-dose exposures of cells to ALA-PDT resulted in significant cell proliferation..…”

mentioning

confidence: 96%

Thioredoxins in cardiovascular disease

Whayne

Parinandi

Maulik

2015

Can. J. Physiol. Pharmacol.

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Key thioredoxin (Trx) system components are nicotinamide adenine dinucleotide phosphate (NADPH), Trx reductase (TrxR), and Trx. TrxR catalyzes disulfide reduction in Trx with NADPH as cofactor. Because Trx is an antioxidant, oxidative stress results in an increase in Trx, which has a reduced disulfide component. If Trx is suppressed, oxidative stress in higher.In contrast a decrease in oxidative stress is associated with low Trx levels. Trx is involved in inflammation, apoptosis, embryogenesis, and cardiovascular disease (CVD). This review focuses on the Trx system in CVD. Abnormal Trx binding occurs in mouse familial combined hyperlipidemia; however, this has not been confirmed in humans. Congestive heart failure is a manifestation of many CVDs, which may be improved by attenuating oxidative stress through the suppression of Trx and decreased reactive oxygen species. Angiotensin II is associated with hypertension and other CVDs and its receptor blockade results in decreased oxidative stress with reduced Trx levels. Inflammation is a major causative factor of CVDs and myocarditis as an example, is associated with increased Trx levels. Vascular endothelial dysfunction has an association with CVD. This dysfunction is alleviated by hormone replacement therapy, which involves decreased oxidative stress and Trx levels. Diabetes mellitus has a major association with CVDs; increase in Trx levels may reflect insulin resistance. Identification of Trx system abnormalities may lead to innovative approaches to treat multiple CVDs and other pathologies.

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Thioredoxin upregulation by 5‐aminolaevulinic acid‐based photodynamic therapy in human skin squamous cell carcinoma cell line

Abstract: Low-dose exposure ALA-PDT increased the expression of thioredoxin and facilitated the growth of HSC-5 cells.

Cited by 7 publications

References 31 publications

Approaches to improve photodynamic therapy of cancer

Approaches to improve photodynamic therapy of cancer

Au Nanoclusters and Photosensitizer Dual Loaded Spatiotemporal Controllable Liposomal Nanocomposites Enhance Tumor Photodynamic Therapy Effect by Inhibiting Thioredoxin Reductase

Thioredoxins in cardiovascular disease

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