Thickness of Acquired Salivary Pellicle as a Determinant of the Sites of Dental Erosion

Abstract

Dental erosion shows a typical distribution pattern within the dental arches. Tooth protection from erosion by salivary pellicle has been shown in vitro, but the hypothesis that pellicle may differ quantitatively at sites of erosion has not been investigated. This study aimed to determine the thickness of acquired salivary pellicle within the dental arches, investigate the possible relationship of this thickness to the distribution and severity of erosion within the arches, and confirm the protective effect of pellicle against dental erosion. Eight enamel blocks were produced from each of 5 bovine incisors assigned to five volunteers. Each block was further cut into 2 slabs, producing control and experimental slabs. Pellicle developed on experimental slabs located on 8 intra-oral sites after 1 hr of exposure was stained by "sheep anti-human IgGAM-FITC". Slabs were then visualized, and pellicle thickness measured, by confocal laser scanning microscopy. Eroded enamel lesions were produced in experimental and control slabs by means of pure orange juice. The degree of erosion was quantified by transverse microradiography. Pellicle thickness varied significantly within the dental arches and among individuals. An inverse relationship (r = -0.96, p<0.001) was observed between the degree of erosion and pellicle thickness. Significant differences in erosion were observed between slabs with and those without pellicle. This study has shown that the thickness of acquired salivary pellicle varies within the dental arches, which may be responsible for the site-specificity of dental erosion, and that pellicle does protect the teeth from erosion.

Prevention. Part 3: Prevention of tooth wear

“…52 Saliva and pellicle are important factors in protection of tooth substance against acid attack. Amaechi et al 33 and Johansson et al 47 have shown that erosion is usually found in areas of the dental arches that are lacking in pellicle. Increasing salivary flow and hence accumulation of pellicle will, therefore, probably offer protection against erosion.…”

“…[29][30][31] Addition of fluoride to sports drinks has also been shown to reduce the erosive potential of these, otherwise highly erosive, drinks. 32 Amaechi et al, 33 have shown that xylitol and fluoride have an additive effect in reducing the erosive potential of orange juice in in vitro studies. Larsen, 34 however, showed that the protective effect against erosion of fluoride added to soft drinks was minimal.…”

Influência da frequência de aplicação da solução de AmF/NaF/SnCl2 no seu potencial em inibir a progressão da lesão de erosão no esmalte dental humano

“…Estudos sugerem que as superfícies dos dentes com películas mais finas têm uma resistência menor à dissolução mineral em comparação com as superfícies dos dentes com películas mais espessas (Amaechi et al, 1999;Hannig e Balz, 1999;Khan, 2002). Em indivíduos susceptíveis à erosão dental a película adquirida alterada pode ser um potencial fator de risco que contribui para o desenvolvimento de lesões erosivas Moazzez et al, 2014).…”

Determinação da mudança na composição da película adquirida formada sobre esmalte e dentina após exposição ácida: estudo proteômico

“…Em relação à erosão dentária, a película adquirida pode proteger por atuar como uma barreira de difusão ou membrana, prevenindo o contato direto entre os ácidos e a superfície dentária, desta maneira reduzindo a dissolução dos tecidos dentários duros (AMAECHI et al, 1999;BALZ, 1999;HANNIG, M. et al, 2004;HARA et al, 2006). Em adição, a película é também uma base para que as bactérias possam aderir sobre a superfície dentária quando entram na cavidade bucal (LENANDER-LUMIKARI; LOIMARANTA, 2000), interferindo, desta maneira, na ocorrência de cárie dentária.…”

“…Quadro 1 -Concentração proteica média (µg/mL) utilizando o micro BCA nas amostras de película adquirida (papel ou líquido) coletadas do esmalte ou dentina, em diferentes tempos da formação (10 minutos e 2 horas), após exposição a diferentes tipos de ácidos 57 1995;ROLLA, 1973). A película lubrifica as superfícies dentárias, o que reduz o atrito entre os dentes e a mucosa oral (evita o desgaste), forma uma barreira contra a erosão, serve como um reservatório de eletrólitos para a remineralização e apresenta várias propriedades antimicrobianas (AMAECHI et al, 1999;HANNIG, M., 2002;HARA et al, 2006;JOINER et al, 2008;GROGAN;OPPENHEIM, 2000).…”