Thiamethoxam at sublethal concentrations induces histopathological, serum biochemical alterations and DNA damage in fish (<i>Labeo rohita)</i>

Hussain, Riaz; Ghaffar, Abdul; Abbas, Ghulam; Jabeen, Ghazala; Khan, Iahtasham; Abbas, Rao Zahid; Noreen, Sobia; Zahid, Iqbal; Chaudhary, Iqra Rasheed; Ishaq, Hafiz Muhammad; Ghori, Muhammad Taslim; Khan, Ahrar

doi:10.1080/15569543.2020.1855655

Cited by 27 publications

(14 citation statements)

References 48 publications

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“…Other possibility of raised oxidative stress in the present study could be the formation of free radicals as a result of toxicity induced by the acetochlor [ 67 , 68 ]. The toxicant-mediated pathways can greatly increase ROS formation and excessive levels of free radicals, which might affect the metabolism [ 69 – 71 ].…”

Section: Discussionmentioning

confidence: 99%

[Retracted] Acetochlor Affects Bighead Carp (Aristichthys Nobilis) by Producing Oxidative Stress, Lowering Tissue Proteins, and Inducing Genotoxicity

Mahmood

Hussain

Ghaffar

et al. 2022

BioMed Research International

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Acetochlor is persistently used in the agroproduction sector to control broadleaf weeds. Due to frequent and continuous applications, this herbicide can reach nearby water bodies and may induce deleterious changes in aquatic life. Therefore, investigation of harmful impacts of different environmental pollutants, including herbicides, is vital to knowing the mechanisms of toxicity and devising control strategies. The current experiment included bighead carp ( n = 80 ) to estimate adverse impacts. Fish were randomly placed in 4 different experimental groups (T0-T3) and were treated for 36 days with acetochlor at 0, 300, 400, and 500 μg/L. Fresh blood without any anticoagulant was obtained and processed for nuclear and morphological changes in erythrocytes. At the same time, various visceral organs, including the gills, liver, brain, and kidneys, were removed and processed on days 12, 24, and 36 to determine oxidative stress and various antioxidant biomarkers. Comet assays revealed significantly increased DNA damage in isolated cells of the liver, kidneys, brain, and gills of treated fish. We recorded increased morphological and nuclear changes ( P ≤ 0.05 ) in the erythrocyte of treated fish. The results on oxidative stress showed a higher quantity of oxidative biomarkers and a significantly ( P ≤ 0.05 ) low concentration of cellular proteins in the gills, liver, brain, and kidneys of treated fish compared to unexposed fish. Our research findings concluded that acetochlor renders oxidative stress in bighead carp.

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Section: Discussionmentioning

confidence: 99%

[Retracted] Acetochlor Affects Bighead Carp (Aristichthys Nobilis) by Producing Oxidative Stress, Lowering Tissue Proteins, and Inducing Genotoxicity

Mahmood

Hussain

Ghaffar

et al. 2022

BioMed Research International

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“…A significant shift in antioxidant enzymes and MDA has also been recorded. In addition, Hussain et al [ 59 ] indicated that TMX significantly increased DNA damage in hepatocytes, kidneys, and blood cells as measured by comet assay in fish.…”

Section: Discussionmentioning

confidence: 99%

Thiamethoxam-induced hematological, biochemical, and genetic alterations and the ameliorated effect of Moringa oleifera in male mice

2022

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“…MX directly interacts with DNA, and has a significant impact on numerous biological and biochemical indices in treated animals Jameel et al (2020). Furthermore, Hussain et al (2020) found that MX increased DNA damage in hepatocytes, kidneys, and blood cells in fish, as determined by comet assay. Recently, observed that giving rats low dosages of imidacloprid for 28 days induced DNA damage in leukocytes and brain cells.…”

Section: Discussionmentioning

confidence: 99%

Deterioration of Cytochrome C Content and Mitochondrial Dysfunction in Brain of Male Rats after Sub-Chronic Exposure to Thiamethoxam and Protective Role of N- Acetylcysteine

Abdel-Razik

Mosallam

Hamed

2022

Alexandria Science Exchange Journal

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Mitochondria sustain healthy brain function. Herein we aimed to evaluate the thiamethoxam (MX) effect on the rat brain mitochondria in addition to the protective role of N-acetylcysteine (NAC) against MX harmful effects. Thiamethoxam was administered orally with five doses each week for 28 days to male albino rats at 1/50 of the LD50 (31.26 mg/kg bw). The results demonstrated that the thiamethoxam neurotoxicity was confirmed by the significant rising in acetylcholinesterase, and lactate dehydrogenase activities of plasma. A significant increase in mitochondrial antioxidants as superoxide dismutase and reduced glutathione was found. Also, significant induction of the oxidative stress and DNA damage via rising the malondialdehyde, and 8-hydroxy-2'-deoxyguanosine biomarkers was recorded by 32.5% and 118.61% respectively. Substantial depression in mitochondrial NADH dehydrogenase, cytochrome c reductase, cytochrome c oxidase, and Mg 2+ ATPase complexes as well as 23 % cerebral infarction was manifested by histological evaluation using the dehydrogenase activity indicator, 2, 3, 5-triphenyl tetrazolium chloride staining. In conclusion, MX can pose a hazard to the integrity and functioning of rats' brain mitochondria, perhaps leading to neurodegenerative disorders. Additionally, earlier treatment of the synthetic antioxidant N-acetylcysteine could prove beneficial in combating the harmful effects of thiamethoxam.

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Thiamethoxam at sublethal concentrations induces histopathological, serum biochemical alterations and DNA damage in fish (Labeo rohita)

Cited by 27 publications

References 48 publications

[Retracted] Acetochlor Affects Bighead Carp (Aristichthys Nobilis) by Producing Oxidative Stress, Lowering Tissue Proteins, and Inducing Genotoxicity

[Retracted] Acetochlor Affects Bighead Carp (Aristichthys Nobilis) by Producing Oxidative Stress, Lowering Tissue Proteins, and Inducing Genotoxicity

Thiamethoxam-induced hematological, biochemical, and genetic alterations and the ameliorated effect of Moringa oleifera in male mice

Deterioration of Cytochrome C Content and Mitochondrial Dysfunction in Brain of Male Rats after Sub-Chronic Exposure to Thiamethoxam and Protective Role of N- Acetylcysteine

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