Thermostable alkaline proteases of Bacillus licheniformis MIR 29: isolation, production and characterization

Ferrero, Marcela Alejandra; Castro, Guillermo R.; Abate, Carlos Mauricio; Baigorí, Mario Domingo; Siñeríz, Faustino

doi:10.1007/s002530050691

Cited by 142 publications

(91 citation statements)

References 16 publications

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“…This result suggests that catabolite repression plays a role in the regulation of alkaline protease expression in NA7. Other reports have indicated similar repressive role of excessive casamino acids on alkaline protease production by other Bacillus strains (Gusek et al, 1988;Ferrero et al, 1996;Gupta et al, 2002;Chauhan and Gupta, 2004). Catabolite repression by simple sugars is also well known in the regulation of protease production in many microorganisms (Sato and Sudo, 1999;Gupta et al, 2002;Sandhya et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge no single report that investigates the expression of alkaline protease by a B. haloalkaliphilus strain has been published. It is well known that protease production by Bacillus cells is directly related to biomass production and is associated with the stationary phase of cell growth (Bascarán et al, 1990;Ferrero et al, 1996;Kumar and Takagi, 1999;Puri et al, 2002;Gupta et al, 2002). The study of NA7 growth kinetics with reference to protease production demonstrated that, prior to the end of the exponential phase, the culture showed marked protease production efficiency with relatively stable maximum activity records (0.610-0.625 TU) for more than 60 h during the stationary phase.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Lotfy¹,

Abd-El-Karim²,

El-Sharouny³

et al. 2017

Afr. J. Biotechnol.

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A Gram-positive, rod-shaped, spore-forming haloalkaliphilic bacterium designated as NA7 was isolated from the surface of a Helianthemum nummularium root sample obtained from Wadi Natrun in Egypt. Sequence analysis of the 16S rRNA gene revealed a Bacillus haloalkaliphilius strain as the closest match with 99% identity. In a shake flask culture containing 10% NaCl, adjusted to pH 10 and incubated at 37°C, the isolated strain produced thermostable extracellular alkaline protease with relatively stable maximum activity records (0.610-0.625 TU) within a relatively long stationary phase that exceeded 60 h. A 2-level fractional factorial design (Plackett-Burman) was then applied to screen for nutritional and cultivation factors regulating protease production by the isolate and to appraise their effects. Calculated statistical parameters revealed that NaCl and MgSO 4 are the most significant independent variables affecting alkaline protease production by NA7 and suggested a near-optimum culture condition. Verification of this predicted condition resulted in an alkaline protease specific activity record of 509 TU/mg protein with a 1.27 fold increase when compared to the basal medium culture.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Lotfy¹,

Abd-El-Karim²,

El-Sharouny³

et al. 2017

Afr. J. Biotechnol.

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show abstract

“…Alkaline proteases of the genus Bacillus generally show an optimal activity and a good stability at high alkaline pH values. The optimum pH range of Bacillus alkaline proteases is generally between 9.0 and 11.0, with a few exceptions of higher pH optimum of 11.5 [122], 11.0-12.0 [30] and 12.0-13.0 [25,26,123,124]. The first report concerning an alkaline protease from alkaliphilic Bacillus strain 221 was published by Horikoshi in 1971.…”

Section: Alkaline Proteases From Alkaliphilesmentioning

confidence: 99%

“…The optimal pH and temperature values of alkaline protease purified from B. licheniformis MIR29 were respectively 12.0 and 60 o C [123]. The alkaline protease of facultative alkaliphilic B. brevis strain was found to be most active at pH 10.5 and 37 o C [28].…”

Section: Alkaline Proteases From Alkaliphilesmentioning

confidence: 99%

Extreme Environments: Goldmine of Industrially Valuable Alkali-stable Proteases

Garg¹,

Singh²

2015

Enz Eng

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Since the advent and release of Bio 40 (the first detergent with bacterial alkaline protease) in 1959, exploration of the microbial alkaline proteases has been exploited beyond expectations. The inventory of microbial alkaline proteases is difficult to draft as it is increasing daily. Most of these proteases are the result of studies in which one or a few isolates were targeted for an alkali-stable enzyme. Although, the worldwide research on microbial alkaline proteases has been widely performed, we still need an improved enzyme capable of catalyzing reactions under extreme conditions (also called as extremozymes), e.g., high alkalinity and salinity, anhydrous environment, cold and hot environment, etc. As the search for extremozymes is in progress, it is imperative to explore the extreme environments to get some novel microbial strains (extremophiles) for alkali-stable proteases. This review article is an effort to compile the scattered information on some extremophiles and their alkali-stable proteases, which may have better specific industrial applications. It includes: (i) various extreme environments relevant to industrial biocatalysts, (ii) strategies of extremophilic microbes and enzymes which make them able to tolerate such conditions, and (iii) an overview of some important work done so far to explore industrially relevant extremophilic enzymes from extremophiles.

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“…On the other hand, extracellular protease activity was observed when crude extracts of Bt strains were electrophoresed on SDS-PAGE containing gelatin powder [50]. The gels were then processed according to [51] for proteolysis to occur. Gel was stained with 0.1% (wt vol -1 ) Coomasie Blue R-250.…”

Section: Assessment Of Enzyme Activities In B Thuringiensismentioning

confidence: 99%

Characterization and Biological Activity of Bacillus thuringiensis Isolates that Are Potentially Useful in Insect Pest Control

Álvarez¹,

Loto²

2012

Biodiversity Enrichment in a Diverse World

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Thermostable alkaline proteases of Bacillus licheniformis MIR 29: isolation, production and characterization

Cited by 142 publications

References 16 publications

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Extreme Environments: Goldmine of Industrially Valuable Alkali-stable Proteases

Characterization and Biological Activity of Bacillus thuringiensis Isolates that Are Potentially Useful in Insect Pest Control

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