Thermophilic bio-energy process study on hydrogen fermentation with vegetable kitchen waste

Lee, Ze-Kun; Li, Shiue-Lin; Kuo, Po-Chih; Chen, I.-Chieh; Tien, Yu-Min; Huang, Yu-Jung; Chuang, Chung-Po; Wong, Shyh-Chyi; Cheng, Sheng-Shung

doi:10.1016/j.ijhydene.2009.11.126

Cited by 65 publications

(28 citation statements)

References 29 publications

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“…About 172 mmol l À1 of lactate and 52 mmol l À1 of acetate were consumed and 79 mmol l À1 of H 2 and 118 mmol l À1 of butyrate were formed. This production also followed reaction (1) as, from a thermodynamic perspective, the most favored pathway for the H 2 production from lactate [18].…”

Section: Experiments At Constant Phmentioning

confidence: 86%

“…However, it has been observed that the presence of Lactobacillus strains significantly reduce the efficiency of H 2 fermentation [13,14], since the reduction of the pH value due to the formation of lactic acid and the antibiotic effect of lactic acid producing bacteria prevent the growth of H 2 producing strains [15,16]. In independent studies, Ohnishi et al [17] and Lee et al [18] both found communities which were able to utilize lactic acid as a substrate for the H 2 fermentation. The bacteria cultivated by Ohnishi et al did not contain a significant number of Clostridia strains, but the Megasphaera elsdenii was assumed to be the H 2 producing species.…”

Section: Introductionmentioning

confidence: 99%

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Lactic acid as a substrate for fermentative hydrogen production

Grause

Igarashi

Kameda

et al. 2012

International Journal of Hydrogen Energy

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Section: Experiments At Constant Phmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Lactic acid as a substrate for fermentative hydrogen production

Grause

Igarashi

Kameda

et al. 2012

International Journal of Hydrogen Energy

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“…Alternatively, anaerobic bacteria, such as Clostridium thermocellum, can also be used for biomass conversion. Recent studies have shown that certain thermophilic anaerobes, such as C. thermocellum strains, have sufficiently high growth and metabolic rates when grown on cellulose to make them competitive with fungi (14,32,34,36,45,55). Many of these anaerobic bacteria utilize high-affinity cellulases organized in stable, membrane-bound, multienzyme cellulosomes, which greatly improve the efficiency of biomass conversion compared to fungi (46,51).…”

mentioning

confidence: 99%

Isolation and Characterization of Shigella flexneri G3, Capable of Effective Cellulosic Saccharification under Mesophilic Conditions

Wang

Gao

Ren

et al. 2011

Appl Environ Microbiol

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A novel Shigella strain (Shigella flexneri G3) showing high cellulolytic activity under mesophilic, anaerobic conditions was isolated and characterized. The bacterium is Gram negative, short rod shaped, and nonmotile and displays effective production of glucose, cellobiose, and other oligosaccharides from cellulose (Avicel PH-101) under optimal conditions (40°C and pH 6.5). Approximately 75% of the cellulose was hydrolyzed in modified ATCC 1191 medium containing 0.3% cellulose, and the oligosaccharide production yield and specific production rate reached 375 mg g Avicel ؊1 and 6.25 mg g Avicel ؊1 h ؊1 , respectively, after a 60-hour incubation. To our knowledge, this represents the highest oligosaccharide yield and specific rate from cellulose for mesophilic bacterial monocultures reported so far. The results demonstrate that S. flexneri G3 is capable of rapid conversion of cellulose to oligosaccharides, with potential biofuel applications under mesophilic conditions.Lignocellulosic biomass is abundant in nature, as well as in agricultural, forestry, and municipal wastes, and can be used as an excellent bioconversion feedstock. Biomass-derived saccharides, such as glucose, cellobiose, and other minor sugars, can be readily fermented by appropriate microbes into bioenergy products, such as hydrogen, ethanol, biodiesel, and other commodity chemicals. However, the high cost of converting biomass to sugars is the primary factor impeding establishment of a cellulosic-biofuels industry (24, 40). Lignocellulosic biofuels can be competitive on an industrial scale if efficient technologies can be developed (29,39). Currently, the most efficient process for utilization of cellulose as a feed stock is either a three-step process (separate hydrolysis and fermentation [SHF]) involving separate pretreatment, cellulose hydrolysis (i.e., saccharification), and hexose and pentose fermentation steps or a two-step process (simultaneous saccharification and fermentation [SSF]) involving separate pretreatment and simultaneous saccharification of hexose and pentose fermentation (48, 64). Combining hydrolysis of cellulose with simultaneous fermentation of hexose and pentose in a single process, i.e., direct microbial conversion (DMC), is an ideal strategy for converting cellulosic biomass to ethanol. However, no single microorganism/community can implement DMC with high efficiency (67). In any of these configurations, rapid and efficient saccharification is critical for developing competitive biotechnologies for cellulosic-biofuel production.In nature, cellulose is hydrolyzed to oligosaccharides by microorganisms, mainly fungi (e.g., brown-, white-, and soft-rot fungi) and bacteria (e.g., Clostridium and Cellulomonas), which produce either free cellulolytic enzymes or extracellular enzyme complexes known as cellulosomes (12). Many white-rot Basidiomycetes and some Actinomycetes have been employed for hydrolyzing lignocellulosic materials. For example, Trichoderma reesei has shown the highest cellulolytic activity currently known (27)....

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“…Acetate (HAc), butyrate (HBu), propionate (HPr), and lactate (HLa) were detected at all tested hydraulic retention times (HRT) as a soluble metabolite by-product, as shown in Lee et al, (2010) who found that the soluble metabolite byproducts increased proportionally with OLR up to a certain limit using I-CSTR. Further OLR increases beyond this limit decrease the soluble metabolite by-product.…”

Section: Metabolite By-productmentioning

confidence: 85%

“…The COD removal (%) deterioration associated with the negative effects on the fermentation process, was related to reducing the time span of substrate hydrolysis. Furthermore, a higher loading and a shorter HRT, inhibited and/or overloaded the process Lee et al, (2010). found that COD degradation efficiency decreased from 24 to 9 % at increasing OLR from 19 to 28 g COD /L/d in an intermittent-continuously stirred tank reactor (I-CSTR) fed with vegetable kitchen waste as the sole substrate.…”

mentioning

confidence: 99%