Thermoactive metallo-keratinase from Bacillus sp. NFH5: Characterization, structural elucidation, and potential application as detergent additive

Kokwe, Lupho; Nnolim, Nonso E.; Ezeogu, Lewis I.; Sitholé, Bruce; Nwodo, Uchechukwu U.

doi:10.1016/j.heliyon.2023.e13635

Cited by 9 publications

(9 citation statements)

References 81 publications

(113 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 25 ] Proteases, especially from Bacillus species, are vital alkaline enzymes widely studied for industrial use. [ 6,9,11,12,26 ] In the present study, the majority of alkaliphilic and proteolytic isolates from Lake Van were Bacillus species. However, E. alkaliphilum VLP1 displayed superior proteolytic activity than the other isolates (Figure S1).…”

Section: Resultsmentioning

confidence: 55%

“…Development of environmentally friendly cleaners has become more important in recent years in order to reduce the use of detergents containing many synthetic chemicals such as surfactants, phosphates, chlorine, and bleach, which can harm human health, aquatic life, soil and other ecosystems. [2,11,12] Enzymes also enable effective cleaning in short wash cycles, avoiding the need for repeated washes and ensuring deep cleaning. Enzymes, derived from renewable sources, are non-toxic and biodegradable, supporting environmentally conscious cleaning practices.…”

Section: Washing Performance Of E Alkaliphilum Vlp1 Protease As a Det...mentioning

confidence: 99%

“…[ 7,9 ] Bacillus species are the preferred source of proteases for use in the detergent industry due to their ability to grow and produce large quantities of protease in extremely alkaline conditions. [ 9–12 ] Still, proteases from novel sources adapted to extreme conditions continue to be screened. [ 13,14 ]…”

Section: Introductionmentioning

confidence: 99%

“…[7,9] Bacillus species are the preferred source of proteases for use in the detergent industry due to their ability to grow and produce large quantities of protease in extremely alkaline conditions. [9][10][11][12] Still, proteases from novel sources adapted to extreme conditions continue to be screened. [13,14] Soda lakes, considered extreme habitats in terms of pH, are distinguished from other lakes by their high carbonate and salt content.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of a novel purified and characterized alkaline protease from the extremophile Exiguobacterium alkaliphilum VLP1 as a detergent additive

Hemsinli,

Gurkok

2023

Biotechnology Journal

View full text Add to dashboard Cite

This study focused on the isolation and identification of a novel alkaline protease‐producing strain from Lake Van, the largest soda lake on Earth. The objective was to purify, characterize, and investigate the potential application of protease in the detergent industry. Through a combination of classical and molecular methods, the most potent protease producer was identified as Exiguobacterium alkaliphilum VLP1. The purification process, involving ammonium sulfate precipitation, ultrafiltration, and anion exchange chromatography, resulted in a 45‐fold purification with a yield of 6.4% and specific activity of 1169 U mg−1 protein. The enzyme exhibited a molecular weight of 69 kDa, a Km value of 0.4 mm, and a maximal velocity (Vmax) value of 2000 U mg−1. The optimum activity was observed at 40°C and potential of hydrogen (pH) 9, while the enzyme also exhibited remarkable stability in the ranges of 30–60°C and pH 9–12. Notably, this study represents the first report of an alkaline protease isolated and characterized from E. alkaliphilum. This study also highlighted the potential of the enzyme as a detergent additive, as it showed compatibility with commercial detergents and effectively removed blood and chocolate stains from fabrics.

show abstract

Section: Resultsmentioning

confidence: 55%

Section: Washing Performance Of E Alkaliphilum Vlp1 Protease As a Det...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…[7,9] Bacillus species are the preferred source of proteases for use in the detergent industry due to their ability to grow and produce large quantities of protease in extremely alkaline conditions. [9][10][11][12] Still, proteases from novel sources adapted to extreme conditions continue to be screened. [13,14] Soda lakes, considered extreme habitats in terms of pH, are distinguished from other lakes by their high carbonate and salt content.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of a novel purified and characterized alkaline protease from the extremophile Exiguobacterium alkaliphilum VLP1 as a detergent additive

Hemsinli,

Gurkok

2023

Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…It is observed that the enzyme maintained its stability very well in the presence of commercial detergents, similar to strawberry protease. [ 47 ] In a study examining the potential of Bacillus cereus YQ15 keratinase as a detergent additive, researchers investigated the effects of different commercial detergents known in the local market on enzyme activity. According to the reported results, the residual activity of the enzyme as a result of incubation with seven different detergents separately at 50 °C for 30 min varied by 60% and above.…”

Section: Resultsmentioning

confidence: 99%

Strawberry Protease as a Laundry Detergent Additive Candidate: Immobilization, Compatibility Study with Detergent Ingredients, and Washing Performance Test

Alici,

Arabaci

2023

Global Challenges

View full text Add to dashboard Cite

The potential of strawberry‐derived protease as a component of laundry detergent is investigated. The compatibility of the enzyme with various surfactants, oxidizing agents, and commercial detergents is tested. The immobilized enzyme prepared by immobilizing Co2+ ions together with the enzyme is also tested. Strawberry crude protease shows high stability in the presence of surfactants frequently used in detergents. The enzyme is found to be relatively stable to oxidizing agents. In addition, it is determined that strawberry protease works in excellent compatibility with different commercial solid and liquid detergents in the Turkish market and also maintains its stability very well. Washing tests based on visual examination also reveal that the enzyme improves the washing performance of the tested detergent. All these properties and high activity at alkaline pH make this enzyme a very strong candidate for use in laundry detergent formulations.

show abstract

Thermostable keratinase from Bacillus cereus L10: optimization and some potential biotechnological applications

Derhab,

Mabrouk,

El-Metwally

et al. 2023

Biomass Conv. Bioref.

View full text Add to dashboard Cite

The accumulation of feather waste is a critical problem in solid waste management; therefore, microbial management of waste is highly significant. Consequently, the keratinolytic capacity of some bacteria isolated from the keratinous waste area was evaluated. The most promising isolate coded as L10 showed the highest feather degradation efficiency 72.7% was identified as Bacillus cereus L10 using 16S rRNA gene sequencing. The effect and contribution of independent process variables were studied by Plackett–Burman design. The optimal values of the independent variables obtained from the Plackett–Burman design were chicken feather, 1%; K2HPO4, 0.3%; KH2PO4, 0.05%; MgSO4 7H2O, 0.01%; yeast extract, 0.05%; inoculum size 4% with pH 7 and incubation period 5 days at 35 °C. The optimal parameters were validated resulting in the enhancement of keratinase production (9.602 U/ml), protein concentration (521.17 µg/ml), and feather degradation (94%) along with a 4.56-fold increased of keratinase production. The enzyme is relatively thermostable, completely stable between 30 and 80 °C for 1 h and retained 97.66% of its activity at 100 °C. B. cereus L10 was able to degrade different types of keratins. Interestingly, the feather hydrolysate from optimized keratinase production medium showed plant growth promoting activity by producing indole-3-acetic acid (IAA). The feathers hydrolysate produced a great benefit by its use as a soil biofertilizer through enhancing the germination of Triticum aestivum. The important implication of keratinase in laundry detergent and leather industry was examined. Results revealed that the enzyme achieved great efficiency in removing protein and blood-rich stains within 30 min at 36 °C in addition to its thoroughly dehairing of cow-hide after 40 h incubation without any damage. The dehaired pelt exhibited a smooth, velvety, and white color surface, good flexibility. Therefore, our study presents an effective method to turn waste into wealth. Graphical Abstract

show abstract

Thermoactive metallo-keratinase from Bacillus sp. NFH5: Characterization, structural elucidation, and potential application as detergent additive

Cited by 9 publications

References 81 publications

Evaluation of a novel purified and characterized alkaline protease from the extremophile Exiguobacterium alkaliphilum VLP1 as a detergent additive

Evaluation of a novel purified and characterized alkaline protease from the extremophile Exiguobacterium alkaliphilum VLP1 as a detergent additive

Strawberry Protease as a Laundry Detergent Additive Candidate: Immobilization, Compatibility Study with Detergent Ingredients, and Washing Performance Test

Thermostable keratinase from Bacillus cereus L10: optimization and some potential biotechnological applications

Contact Info

Product

Resources

About