“…An important requirement for these systems is the ability to rapidly and efficiently mix small amounts of samples at microscales 15,66 . Various techniques have been utilized to enable rapid mixing in microfluidic devices including chaotic advection 13,14,26,33,40,62 , hydrodynamic focusing 45,51 , electrokinetically driven mixing 16,23,32,48,60 , 3D combinatorial bubble-based mixing 38,47 , and thermally- 63,69 as well as optically-induced 25 mixing. Recently, acoustic-based mixers 39,43,52,59,64 have generated significant interest because of their non-invasive nature.…”