Thermal inactivation of Cronobacter sakazakii ATCC 29544 in powdered infant formula milk using thermostatic radio frequency

Zhang, Yue; Xie, Yongkang; Tang, Juming; Wang, Shaojin; Wang, Lu; Zhu, Guangfei; Li, Xingyi; Liu, Yanhong

doi:10.1016/j.foodcont.2020.107270

Cited by 31 publications

(13 citation statements)

References 48 publications

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“…Results both showed similar gene response and cell morphology changes, indicating that causes of microbial inactivation are similar after RF pasteurisation and traditional heat treatment. Similar finding was also reported by Zhang et al (2020c). Some valuable insights about mechanisms of pathogens pasteurised by RF heating were provided by these findings.…”

Section: Pasteurisation Mechanism Of Rf Heatingsupporting

confidence: 90%

“…The pasteurisation mechanism with RF heating for inactivating microorganisms are generally considered as thermal effects (Kou et al ., 2018; Zhang et al ., 2020c). However, a few research reports still claimed that non‐thermal effects during RF heating existed (Saadi et al ., 2014).…”

Section: Applications Of Rf Pasteurisation Treatment For Seedsmentioning

confidence: 99%

“…Similar finding was also reported by Zhang et al . (2020c). Some valuable insights about mechanisms of pathogens pasteurised by RF heating were provided by these findings.…”

Section: Applications Of Rf Pasteurisation Treatment For Seedsmentioning

confidence: 99%

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Recent developments in pasteurising seeds and their products using radio frequency heating: a review

Guan

Lin

et al. 2021

Int J of Food Sci Tech

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Food-borne or plant diseases mainly rely on the spread of seeds with pathogens. It is vital to eliminate food/seed-borne pathogens of seeds and their products before commercial import/export or circulation. Radio frequency (RF) heating is identified as an effective method with lack of chemical residues, rapid or volumetric heating and large penetration depth, and also validated as a potential physical method to eliminate pests or pathogens in agricultural products or foods. Although, many reviews have summarised the applications of RF treatment during the process of foods, little comprehensive review has been focused on seeds and their products pasteurised by the RF energy. Comprehensive summary about principles and main applications of RF energy for pasteurising seeds and their products was reported in this review. This review also introduced some typical RF heating systems, thermal death kinetics of pathogens, influences of RF heating on germination rate of seeds and some ways for improving uniformity of RF heating. Furthermore, this review recommended the future research directions about treatment of seeds and their products using RF energy, especially for seed industry to control the pathogens in seeds without reducing their quality after RF treatments.

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Section: Pasteurisation Mechanism Of Rf Heatingsupporting

confidence: 90%

Section: Applications Of Rf Pasteurisation Treatment For Seedsmentioning

confidence: 99%

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Recent developments in pasteurising seeds and their products using radio frequency heating: a review

Guan

Lin

et al. 2021

Int J of Food Sci Tech

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“…However, of these metabolites, only acetate was negatively correlated with C. sakazakii. Acetate, in addition to propionate and butyrate, has been reported to reduce the growth and pathogenicity of foodborne pathogens (Peng and Biswas, 2017;Zhang et al, 2020). In this study, however, only acetate was shown to be a significant factor in reducing the levels of C. sakazakii.…”

Section: Discussioncontrasting

confidence: 55%

Inhibition of Cronobacter sakazakii in an infant simulator of the human intestinal microbial ecosystem using a potential synbiotic

Parreira²,

Farber³

et al. 2022

Front. Microbiol.

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Powdered infant formula (PIF) can be contaminated with Cronobacter sakazakii, which can cause severe illnesses in infants. Synbiotics, a combination of probiotics and prebiotics, could act as an alternative control measure for C. sakazakii contamination in PIF and within the infant gut, but synbiotics have not been well studied for their ability to inhibit C. sakazakii. Using a Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) inoculated with infant fecal matter, we demonstrated that a potential synbiotic, consisting of six lactic acid bacteria (LAB) strains and Vivinal GOS, can inhibit the growth of C. sakazakii in an infant possibly through either the production of antimicrobial metabolites like acetate, increasing species diversity within the SHIME compartments to compete for nutrients or a combination of mechanisms. Using a triple SHIME set-up, i.e., three identical SHIME compartments, the first SHIME (SHIME 1) was designated as the control SHIME in the absence of a treatment, whereas SHIME 2 and 3 were the treated SHIME over 2, 1-week treatment periods. The addition of the potential synbiotic (LAB + VGOS) resulted in a significant decrease in C. sakazakii levels within 1 week (p < 0.05), but in the absence of a treatment the significant decline took 2 weeks (p < 0.05), and the LAB treatment did not decrease C. sakazakii levels (p ≥ 0.05). The principal component analysis showed a distinction between metabolomic profiles for the control and LAB treatment, but similar profiles for the LAB + VGOS treatment. The addition of the potential synbiotic (LAB + VGOS) in the first treatment period slightly increased species diversity (p ≥ 0.05) compared to the control and LAB, which may have had an effect on the survival of C. sakazakii throughout the treatment period. Our results also revealed that the relative abundance of Bifidobacterium was negatively correlated with Cronobacter when no treatments were added (ρ = −0.96; p < 0.05). These findings suggest that C. sakazakii could be inhibited by the native gut microbiota, and inhibition can be accelerated by the potential synbiotic treatment.

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“…Contamination with C. sakazakii usually occurs during the post-pasteurization packaging or adding ingredients to the infant formula foods [23]. The thermal resistance of this pathogen is significantly higher than that of other Enterobacteriaceae species [30]. Stabilized membrane proteins and phospholipids and trehalose production and accumulation by C. sakazakii protect this pathogen from the dry conditions in low-moisture foods such as infant formula and improve its thermal resistance.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance and Molecular Characterization of Cronobacter sakazakii Strains Isolated from Powdered Infant Formula Milk

Pakbin

Brück²,

Allahyari

et al. 2022

Foods

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Background: Cronobacter sakazakii is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of C. sakazakii strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the C. sakazakii isolates. Results: We isolated 25 C. sakazakii strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant C. sakazakii are required to control the contamination of this foodborne pathogen in infant foods.

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Thermal inactivation of Cronobacter sakazakii ATCC 29544 in powdered infant formula milk using thermostatic radio frequency

Cited by 31 publications

References 48 publications

Recent developments in pasteurising seeds and their products using radio frequency heating: a review

Recent developments in pasteurising seeds and their products using radio frequency heating: a review

Inhibition of Cronobacter sakazakii in an infant simulator of the human intestinal microbial ecosystem using a potential synbiotic

Antibiotic Resistance and Molecular Characterization of Cronobacter sakazakii Strains Isolated from Powdered Infant Formula Milk

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