Therapeutic effects of Nigella sativa on hormonal dysfunctions

Wani, Javaid Ahmad; Tsagkaris, Christos; Majid, Sabhiya; Ganie, Majid A.; Akhter, Rehana; Ahmad, Sheikh Bilal; Ovais, Syed; Khan, Mosin Saleem; Wani, Hilal Ahmad

doi:10.1016/b978-0-12-824462-3.00001-9

Cited by 5 publications

(8 citation statements)

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“…In addition, the antioxidant defense of TQ against the generation of ROS might explain its efficacy in protecting the testicular tissue (Negi et al, 2018). Recently, TQ was also reported to block the release of apoptotic mediators and inflammatory cytokines, as was evident in our study, and subsequently inhibit the inflammatory process and apoptosis in the testicular tissue (Wani et al, 2022).…”

Section: Figuresupporting

confidence: 80%

“…Reduced TH level is described to be associated with reduction in the antioxidant defense with subsequent loss of testicular defense mechanism against ROS produced by the cellular metabolism ( Sahoo et al, 2008 ). Thymoquinone (TQ), a prominent bioactive flavonoid of Nigella sativa , has a highly potent testicular protective effect via its antioxidant, anti-inflammatory, antiapoptotic, fertility-enhancing, and endocrine and immune-modulatory effects ( Hassan et al, 2019 ; Wani et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Thymoquinone protects the testes of hypothyroid rats by suppressing pro-inflammatory cytokines and oxidative stress and promoting SIRT1 testicular expression

et al. 2022

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Background: Hypothyroidism has been linked to many testicular structural and dysfunctional changes in males. Thymoquinone (TQ) has shown a potent testicular protective effect through its antioxidant, anti-inflammatory, antiapoptotic, fertility-enhancing, and endocrine modulatory activities.Objectives: This study aimed to investigate the efficacy of TQ in preserving the testicular structure of a model of experimentally induced hypothyroidism in rats and identify the mechanism behind this effect.Materials and methods: Propylthiouracil (PTU) was used to induce hypothyroidism in adult male Wistar rats, who were then treated with TQ (50 mg/kg/body weight) for 4 weeks and compared to the untreated rats. Thyroid hormonal profile, oxidants/antioxidants profile, and serum testosterone levels were assessed. Gene expression and immune expression of SIRT1 and pro-inflammatory cytokines TNF-α and NF-κB were also assessed in the testicular tissue.Results: TQ administration successfully improved PTU-induced disturbance in the thyroid hormonal profile (T3, T4, and TSH), serum testosterone level, and pancreatic antioxidants compared to the untreated hypothyroid group. TQ significantly downregulated (p = 0.001, p ˂ 0.001) TNF-α and NF-κB transcription, while it significantly upregulated (p = 0.01) SIRT1 transcription in the testes of hypothyroid rats. TQ markedly relieved the histopathological testicular changes induced by PTU and significantly increased (p = 0.002, p = 0.01) the sectional area of seminiferous tubules and germinal epithelial height, respectively. TUNEL-positive apoptotic germinal cells were significantly decreased (p ˂ 0.001), while PCNA-positive proliferating germinal cells and androgen receptor expression were significantly increased (p ˂ 0.001) in the testes of TQ-treated hypothyroid rats.Conclusion: Thymoquinone could limit the hypothyroidism-induced structural changes in the testis, mostly through the upregulation of SIRT1 expression, which seems to mediate its promising antioxidant, anti-inflammatory and antiapoptotic effects that were evident in this study. Therefore, TQ is recommended as an adjuvant safe supplement in managing hypothyroidism, especially in males.

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Section: Figuresupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Thymoquinone protects the testes of hypothyroid rats by suppressing pro-inflammatory cytokines and oxidative stress and promoting SIRT1 testicular expression

et al. 2022

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“…It also promoted sex hormone levels and corrected the sexual hormone imbalance both in vitro and in vivo by upregulating aromatase gene expression and reducing oxidative stress by elevating the levels of antioxidant enzymes. The study proved that NSO had therapeutic effects against PbO toxicity in rats (Wani et al, 2022). These results are in line with a previous study, where NSO at 250 mg/kg was reported to increase the level of testosterone in rats exposed to lead acetate (Assi et al, 2016).…”

Section: Discussionsupporting

confidence: 92%

“…Thymoquinone can protect against testicular tissueinduced damage through an antioxidant mechanism (Mollazadeh & Hosseinzadeh, 2014). This compound inhibits the inflammation process and apoptosis within the testicular tissue by blocking the release of apoptotic mediators and inflammatory cytokines such as cytochrome C and Bax proteins (Wani et al, 2022). These findings agree with the remarks that show PbO acts as a spermicidal factor, leading to the suppression of serum reproductive hormone concentration and spermatogenesis in rats.…”

Section: Discussionsupporting

confidence: 90%

EFFICACY OF Nigella sativa OIL TO RELIEVE EFFECTS OF THE LEAD MONOXIDE TOXICITY ON TESTICULAR EFFICIENCY AND SEXUAL BEHAVIOUR DISORDERS IN ALBINO RATS

Chabuk

Awadh

2022

MABJ

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The prevalence of testicular toxicity induced via contamination with heavy metals is increasing worldwide, and a close connection between testicular toxicity and lead has recently been identified. The present study demonstrated the possible therapeutic and protection properties of Nigella sativa oil (NSO) against dangerous testicular toxicity and sexual behaviour disorders in rat models induced by lead monoxide (PbO) food poisoning. A total of 24 male rats were used and divided into four groups, consisting of Group 1: Control (1 mL of distilled water); Group 2: PbO (80 mg/kg); Group 3: NSO (0.5 mL/rat); and Group 4: (NSO+PbO) 0.5 mL/rat of NSO, followed by PbO (80 mg/kg/day) respectively for 60 days. The testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), 17 beta-hydroxysteroid dehydrogenase activity (17β-HSD), sperm parameters, and sexual behaviour (copulation behaviour test) were measured. The results showed that sex hormone levels were significantly decreased (p<0.05) in rats exposed to PbO, while the levels of these hormones were significantly higher in rats exposed to NSO and NSO+PbO. The sperm parameters (numbers, concentrations, motilities, and abnormality) were lower in rats exposed to PbO compared to those in the control, NSO, and NSO+PbO groups that showed significant increases. Sperm abnormality and 17β-HSD activity showed a significant increase at p<0.05 in the group exposed to the PbO compared to those of the control, NSO, and NSO+PbO groups. The results also significantly suppressed sexual behaviour in rats exposed to PbO compared to those in the control, NSO, and NSO+PbO groups, rats were administered NSO+PbO showed improvement in sexual behaviour parameters compared to PbO groups. PbO toxicity was obvious in the reproductive tract through severe histopathological changes. The impact of lead on tissues was partially mitigated by NSO. The study showed protective effects of N. sativa oil against changes in sex hormones, sperm characteristics, and sexual behaviour in male rats exposed to lead monoxide-induced testicular toxicity.

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“…Researchers have identified that a lot of the pharmacological activities of Nigella sativa are credited to the most active nutrient—thymoquinone a bioactive flavonoid (Tavakkoli et al, 2017). The reported pharmacological activities of the plant include but are not limited to antioxidant (Iqbal et al, 2021), anti‐inflammatory (Bordoni et al, 2019), immunomodulatory (Khazdair et al, 2021; Shaterzadeh‐Yazdi et al, 2018), neuroprotective (Fanoudi et al, 2019; Landucci et al, 2021) and fertility‐enhancing (Razak et al, 2017; Shikhbahaei et al, 2018; Wani et al, 2022) capacities, among others. Hence, this experiment aims to examine the probable protective potential of thymoquinone against cyclophosphamide‐induced testicular toxicity using animal models.…”

Section: Introductionmentioning

confidence: 99%

Oral thymoquinone modulates cyclophosphamide‐induced testicular toxicity in adolescent Wistar rats

et al. 2022

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Cyclophosphamide (CYP) is an effective anti‐cancer drug that is widely accepted, but it is not devoid of unintended toxic effects. Gonadal toxicity is reported as one of the side effects of its long‐time use. This study examined the effects of thymoquinone (TQ) on the biological integrities of the testes after cyclophosphamide exposure. Thirty adolescent male Wistar rats (100–110 g) were divided into six groups (n = 5), receiving normal saline (NS), 20 mg/kg of CYP (CYP), 5 mg/kg of TQ (TQ5), 10 mg/kg of TQ (TQ10), 20 mg/kg of CYP and 5 mg/kg of TQ (CTQ5), and 20 mg/kg of CYP and 10 mg/kg of TQ (CTQ10) respectively. On the 22nd day, blood, semen and testicular samples were collected for the assay of serum reproductive hormones (follicle‐stimulating (FSH) and luteinizing (LH) hormones), semen analysis and testicular histology and proliferating cell nuclear antigen (PCNA) expression. The results revealed that CYP exposure affected functional and structural integrities of the testes, by depleting sperm count and motility, testosterone, LH, spermatogenic and mature sperm cell population, Leydig cells and PCNA immunoreactive proliferating cells. TQ interventions were able to reverse all cytotoxic CYP impacts, but with differential activities on the hormonal concentrations, specifically LH and FSH. Cumulatively, thymoquinone may be a potent agent against cyclophosphamide effects on the physiological, regeneration and histological integrities of the testes, as observed in this study.

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Therapeutic effects of Nigella sativa on hormonal dysfunctions

Cited by 5 publications

References 50 publications

Thymoquinone protects the testes of hypothyroid rats by suppressing pro-inflammatory cytokines and oxidative stress and promoting SIRT1 testicular expression

Thymoquinone protects the testes of hypothyroid rats by suppressing pro-inflammatory cytokines and oxidative stress and promoting SIRT1 testicular expression

EFFICACY OF Nigella sativa OIL TO RELIEVE EFFECTS OF THE LEAD MONOXIDE TOXICITY ON TESTICULAR EFFICIENCY AND SEXUAL BEHAVIOUR DISORDERS IN ALBINO RATS

Oral thymoquinone modulates cyclophosphamide‐induced testicular toxicity in adolescent Wistar rats

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