Theoretical investigations of a modified compressed ultrafast photography method suitable for single-shot fluorescence lifetime imaging

Abstract

A single-shot fluorescence lifetime imaging (FLIM) method based on the compressed ultrafast photography (CUP) is proposed, named space-restricted CUP (srCUP). srCUP is suitable for imaging objects moving slowly (< ∼ 150/M mm/s, M is the magnification of the objective lens) in the field of view with the intensity changing within nanoseconds in a measurement window around 10 ns. We used synthetic datasets to explore the performances of srCUP compared with CUP and TCUP (a variant of CUP). srCUP not only provides superior reconstruction performances, but its reconstruction time is also two-and threefold faster than CUP and TCUP, respectively. The lifetime determination performances were assessed by estimating lifetime components, amplitude-and intensity-weighted average lifetimes (τ A and τ I ) with the reconstructed scenes using the least square method based on a bi-exponential model. srCUP has the best accuracy and precision for lifetime determinations with a relative bias less than 7% and a coefficient of variation less than 7% for τ A and a relative bias less than 10% and a coefficient of variation less than 11% for τ I .

Design and Study of a Two-Dimensional (2D) All-Optical Spatial Mapping Module

“…All-optical mapping photography [1][2][3] has the advantages of high time resolution, high luminous flux, and anti-radiation interference and can effectively overcome the shortcomings of the pump-probe technique [4,5], which is unable to perform non-repeatable or difficult-to-replicate event observation. Meanwhile, it can also avoid the drawbacks of low spatial resolution and complex image reconstruction from compressed ultra-fast spectroscopy [6,7] and multi-spectral tomography [8]. This technology has significant application prospects in the fields of observation and analysis of events at the picosecond and even femtosecond scale, such as shockwave dynamics, engine tail flame detection, superconducting electronic states, structural dynamics of chemical reactions, protein folding, and the process of photosynthesis [9][10][11][12][13][14].…”