ThehapC gene ofAspergillus nidulans is involved in the expression of CCAAT-containing promoters

Papagiannopoulos, Peter; Andrianopoulos, Alex; Sharp, Julie A.; Ma, Duan; Mj, Hynes

doi:10.1007/bf02172369

Cited by 49 publications

(65 citation statements)

References 41 publications

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“…Subsequently, Kennedy and Turner (32) demonstrated that the expression of acvA, another gene in the PN cluster encoding ␦-(L-␣-aminoadipyl)-L-cysteinyl-D-valine synthetase, is the rate-limiting step in PN biosynthesis. Furthermore, a reduction in ipnA caused by deletion of the hapC gene (which encodes a component of the wide-domain-regulatory CCAAT-binding complex AnCF/AnCP/ PENR1 [28,37,40]) resulted in only a moderate reduction in PN, since acvA expression was only slightly affected by the deletion (37). Our results showed a correlation between acvA expression levels and the bioassay results ( Fig.…”

Section: Discussionmentioning

confidence: 51%

The Expression of Sterigmatocystin and Penicillin Genes in Aspergillus nidulans Is Controlled by veA , a Gene Required for Sexual Development

2003

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Secondary metabolism is commonly associated with morphological development in microorganisms, including fungi. We found that veA, a gene previously shown to control the Aspergillus nidulans sexual/asexual developmental ratio in response to light, also controls secondary metabolism. Specifically, veA regulates the expression of genes implicated in the synthesis of the mycotoxin sterigmatocystin and the antibiotic penicillin. veA is necessary for the expression of the transcription factor aflR, which activates the gene cluster that leads to the production of sterigmatocystin. veA is also necessary for penicillin production. Our results indicated that although veA represses the transcription of the isopenicillin synthetase gene ipnA, it is necessary for the expression of acvA, the key gene in the first step of penicillin biosynthesis, encoding the delta-(L-alphaaminoadipyl)-L-cysteinyl-D-valine synthetase. With respect to the mechanism of veA in directing morphological development, veA has little effect on the expression of the known sexual transcription factors nsdD and steA. However, we found that veA regulates the expression of the asexual transcription factor brlA by modulating the ␣/␤ transcript ratio that controls conidiation.

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Section: Discussionmentioning

confidence: 51%

The Expression of Sterigmatocystin and Penicillin Genes in Aspergillus nidulans Is Controlled by veA , a Gene Required for Sexual Development

2003

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“…PENR1 positively regulates the expression of two penicillin biosynthetic genes, ipnA and aatA. Deletion of hapB, hapC, and hapE genes, which encode PENR1 complex components, resulted in identical phenotypes characterized by low levels of ipnA and aatA expression as well as a slow-growth phenotype and a reduction in conidiation (74,87,112).…”

Section: Ccaat Binding Protein Complexmentioning

confidence: 99%

Relationship between Secondary Metabolism and Fungal Development

Calvo

Wilson

Bok

et al. 2002

Microbiol Mol Biol Rev

932

652

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Filamentous fungi are unique organisms—rivaled only by actinomycetes and plants—in producing a wide range of natural products called secondary metabolites. These compounds are very diverse in structure and perform functions that are not always known. However, most secondary metabolites are produced after the fungus has completed its initial growth phase and is beginning a stage of development represented by the formation of spores. In this review, we describe secondary metabolites produced by fungi that act as sporogenic factors to influence fungal development, are required for spore viability, or are produced at a time in the life cycle that coincides with development. We describe environmental and genetic factors that can influence the production of secondary metabolites. In the case of the filamentous fungus Aspergillus nidulans, we review the only described work that genetically links the sporulation of this fungus to the production of the mycotoxin sterigmatocystin through a shared G-protein signaling pathway

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“…The hapC gene of A. nidulans was identified as a homolog of the HAP3 gene of S. cerevisiae (40). Deletion of this gene resulted in low levels of expression of amdS, aatA, and ipnA reporter gene fusions (28,40).…”

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confidence: 99%

AnCF, the CCAAT Binding Complex of Aspergillus nidulans, Contains Products of the hapB,hapC, and hapE Genes and Is Required for Activation by the Pathway-Specific Regulatory Gene amdR

Steidl

Papagiannopoulos

Litzka

et al. 1999

Molecular and Cellular Biology

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CCAAT binding factors (CBFs) positively regulating the expression of the amdS gene (encoding acetamidase) and two penicillin biosynthesis genes (ipnA and aatA) have been previously found in Aspergillus nidulans. The factors were called AnCF and PENR1, respectively. Deletion of the hapC gene, encoding a protein with significant similarity to Hap3p of Saccharomyces cerevisiae, eliminated both AnCF and PENR1 binding activities. We now report the isolation of the genes hapB and hapE, which encode proteins with central regions of high similarity to Hap2p and Hap5p of S. cerevisiae and to the CBF-B and CBF-C proteins of mammals. An additional fungus-specific domain present in HapE was revealed by comparisons with the homologs from S. cerevisiae, Neurospora crassa, and Schizosaccharomyces pombe. The HapB, HapC, and HapE proteins have been shown to be necessary and sufficient for the formation of a CCAAT binding complex in vitro. Strains with deletions of each of the hapB, hapC, and hapE genes have identical phenotypes of slow growth, poor conidiation, and reduced expression of amdS. Furthermore, induction of amdS by omega amino acids, which is mediated by the AmdR pathway-specific activator, is abolished in the hap deletion mutants, as is growth on ␥-aminobutyric acid as a sole nitrogen or carbon source. AmdR and AnCF bind to overlapping sites in the promoters of the amdS and gatA genes. It is known that AnCF can bind independently of AmdR. We suggest that AnCF binding is required for AmdR binding in vivo.

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ThehapC gene ofAspergillus nidulans is involved in the expression of CCAAT-containing promoters

Cited by 49 publications

References 41 publications

The Expression of Sterigmatocystin and Penicillin Genes in Aspergillus nidulans Is Controlled by veA , a Gene Required for Sexual Development

The Expression of Sterigmatocystin and Penicillin Genes in Aspergillus nidulans Is Controlled by veA , a Gene Required for Sexual Development

Relationship between Secondary Metabolism and Fungal Development

AnCF, the CCAAT Binding Complex of Aspergillus nidulans, Contains Products of the hapB,hapC, and hapE Genes and Is Required for Activation by the Pathway-Specific Regulatory Gene amdR

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