TheBrassica mitochondrial DNA plasmid and large RNAs are not exclusively associated with cytoplasmic male sterility

Kemble, R. J.; Carlson, John E.; Erickson, L.; Sernyk, J. L.; Thompson, D. J.

doi:10.1007/bf02428050

Cited by 38 publications

(9 citation statements)

References 10 publications

(16 reference statements)

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“…Brassica has already been demonstrated [46,24]. The disappearance of this 11.3 kb plasmid via in vitro culture was also recently shown by Erickson et al [ 13].…”

Section: Patterns Of Mtdna Change In Independent Culture Linesmentioning

confidence: 77%

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Patterns of mitochondrial DNA instability in Brassica campestris cultured cells

et al. 1991

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We previously showed that the mitochondrial DNA (mtDNA) of a Brassica campestris callus culture had undergone extensive rearrangements (i.e. large inversions and a duplication) relative to DNA of the control plant [54]. In this study we observed that after continued growth, the mtDNA of this culture continues to change, with rearranged forms amplifying and diminishing to varying proportions. Strikingly similar changes were detected in the mtDNA profiles of a variety of other long- and short-term callus and cell suspension lines. However, the proportions of parental ('unrearranged') and novel ('rearranged') forms varied in different cultured cell mtDNAs. To address the source of this heterogeneity, we compared the mtDNA organization of 28 individual plants from the parental seed stock. With the exception of one plant containing high levels of a novel plasmid-like mtDNA molecule, no significant variation was detected among individual plants and therefore source plant variation is unlikely to have contributed to the diversity of mitochondrial genomes observed in cultured cells. The source of this culture-induced heterogeneity was also investigated in 16 clones derived from single protoplasts. A mixed population of unrearranged and rearranged mtDNA molecules was apparent in each protoclone, suggesting that the observed heterogeneity in various cultures might reflect the genomic composition of each individual cell; however, the induction of an intercellular heterogeneity subsequent to the protoplast isolation was not tested and therefore cannot be ruled out. The results of this study support our earlier model that the rapid structural alteration of B. campestris mtDNA in vitro results from preferential amplification and reassortment of minor pre-existing forms of the genome rather than de novo rearrangement. Infrequent recombination between short dispersed repeated elements is proposed as the underlying mechanism for the formation of these minor mtDNA molecules.

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“…Brassica has already been demonstrated [46,24]. The disappearance of this 11.3 kb plasmid via in vitro culture was also recently shown by Erickson et al [ 13].…”

Section: Patterns Of Mtdna Change In Independent Culture Linesmentioning

confidence: 77%

“…HMV denotes high molecular weight undigested mtDNA. The arrows denote the array of putative RNA molecules that occasionally co-purify with mtDNA [24].…”

Section: Patterns Of Mtdna Change In Independent Culture Linesmentioning

confidence: 99%

Patterns of mitochondrial DNA instability in Brassica campestris cultured cells

et al. 1991

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“…These dsRNAs appear to replicate independently of the cellular DNA (Finnegan and Brown 1986); however, their natural infectivity has never been proved and their biological significance is unknown but of considerable interest. Some dsRNAs are correlated with male sterility of the host plants (Grill and Garger 1981;Scalla et al 1981;Sisco et al 1984), although other authors question a causal connection between the dsRNAs and this phenotype (Kemble et al 1986;Mackenzie et al 1988). …”

Section: Introductionmentioning

confidence: 96%

Association of particles that contain double-stranded RNAs with algal chloroplasts and mitochondria

Ishihara

Pak

Fukuhara

et al. 1992

Planta

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Linear dsRNAs (double-stranded RNAs) belonging to several distinct size classes were found to be localized in chloroplasts and mitochondria of Bryopsis spp., raising the possibility that these dsRNAs are prokaryotic in nature. The algal cytosol and nuclei did not contain dsRNAs. The amount of the dsRNAs in the organelles appeared constant, and there were about 500 copies per chloroplast. The four major dsRNAs from Bryopsis chloroplasts were about 2 kbp (kilobase pairs) in length and originated from discrete isometric particles of about 25 nm diameter. These virus-like particles were purified by CsCl density gradient centrifugation after extraction from isolated chloroplasts with chloroformbutanol and subsequent precipitation with polyethylene glycol. They had a buoyant density of about 1.40 g · cm(-3) and contained four major and three minor proteins. Mitochondrial dsRNAs were about 4.5 kbp in length and formed less-stable particles of about 40 nm in diameter with a buoyant density of 1.47 g · cm(-3). Some observations support the hypothesis that vertical transmission of the protein-coated, non-infectious dsRNAs occurs within cell organelles. Double-stranded RNAs of various sizes were found in most green, red, and brown algae. The characteristics of the algal dsRNAs are compared with those of dsRNAs from higher plants and the biological significance of the dsRNAs in cell organelles is discussed.

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“…However, an extensive analysis using more than 100 different Brassica nucleocytoplasmic combinations later revealed that there was no close association between the presence of this plasmid DNA and the cms trait (Kemble et al 1986). There have been no further reports regarding the biological role of this linear molecule in rapeseed mitochondria to date.…”

Section: Introductionmentioning

confidence: 97%

Structural features and expression analysis of a linear mitochondrial plasmid in rapeseed (Brassica napus L.)

2002

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A linear plasmid molecule about 11 kb in length is present in the mitochondria of some varieties of rapeseed (Brassica napus L.). This plasmid can be inherited from the male parent, through the pollen, as well as by the usual maternal route, although the main mitochondrial genome is maternally inherited in rapeseed. We determined the complete nucleotide sequence of this plasmid DNA and clarified its genetic organization. The length of the linear plasmid is 11,640 bp. At the termini of the plasmid molecule are inverted repeats of 327 bp. The GC content of the plasmid DNA is 30.9%; thus, the plasmid is quite AT-rich compared to the main mitochondrial genome in higher plants. The plasmid has six ORFs, two of which encode a phage-type DNA polymerase and a phage-type RNA polymerase, respectively. RT-PCR analyses revealed that all six ORFs are transcribed, and all four ORFs on the minus strand are probably cotranscribed from a single promoter located in the terminal inverted repeat. We also show here that at least three of the six ORFs are translated into proteins in rapeseed mitochondria, and expressed at relatively high levels in flowers, as shown by Western analysis. These results suggest that this linear DNA molecule is able to replicate as an autonomous replicon and to express the genes it carries in rapeseed mitochondria.

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TheBrassica mitochondrial DNA plasmid and large RNAs are not exclusively associated with cytoplasmic male sterility

Cited by 38 publications

References 10 publications

Patterns of mitochondrial DNA instability in Brassica campestris cultured cells

Patterns of mitochondrial DNA instability in Brassica campestris cultured cells

Association of particles that contain double-stranded RNAs with algal chloroplasts and mitochondria

Structural features and expression analysis of a linear mitochondrial plasmid in rapeseed (Brassica napus L.)

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