The σE Regulon and the Identification of Additional Sporulation Genes in Bacillus subtilis

Eichenberger, Patrick; Jensen, Shane T.; Conlon, Erin M.; Ooij, Christiaan van; Silvaggi, Jessica M.; González-Pastor, José Eduardo; Fujita, Masaya; Ben-Yehuda, Sigal; Stragier, Patrick; Liu, Jun S.; Losick, Richard

doi:10.1016/s0022-2836(03)00205-5

Cited by 214 publications

(310 citation statements)

References 96 publications

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“…Moreover, in addition to SpoIIID and, hypothetically, to YlbO, the expression of some s Edependent genes may be influenced by other factors. Several known or putative transcriptional regulators have been assigned to the s E regulon: purR, encoding the repressor of the purine operons; birA, a biotin acetyl-CoA-carboxylase synthetase and transcriptional regulator (Eichenberger et al, 2003); yhgD (TetR/AcrR family) and ytzE (DeoR family) (Feucht et al, 2003). Note, however, that none of these four genes could be assigned to the s E regulon under our experimental conditions ( Fig.…”

Section: Differentiation Of the Compartment-specific Sporulation Regumentioning

confidence: 81%

“…These lower induction ratios might be a reflection either of the leakiness of the P SPAC promoter or of the only partial activity of the sigma factor alleles used. In the case of sigE, the limited activity of a sigE copy from which pro-amino acid sequences have been removed has been observed before (Eichenberger et al, 2003). Failure to induce the whole set of sporulation genes during growth is not unexpected because many sporulation genes might require other regulatory inputs that are not provided during growth.…”

Section: Differentiation Of the Compartment-specific Sporulation Regumentioning

confidence: 99%

“…Two studies have provided information on the composition of the s E regulon when sporulation is induced by growth and resuspension in a poorer synthetic medium. Eichenberger et al (2003) have reported transcriptional profiling and bioinformatics data to support their assignment of 253 genes to the s E regulon, including 181 new genes. Disruption of 12 of the newly identified genes produced a sporulation phenotype (Eichenberger et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Eichenberger et al (2003) have reported transcriptional profiling and bioinformatics data to support their assignment of 253 genes to the s E regulon, including 181 new genes. Disruption of 12 of the newly identified genes produced a sporulation phenotype (Eichenberger et al, 2003). Feucht et al (2003) have found a total of 171 s Edependent transcripts, 101 of which were previously unknown, and of these, mutations in about 10 diminished the efficiency of sporulation.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis

et al. 2005

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Temporal and compartment-specific control of gene expression during sporulation in Bacillus subtilis is governed by a cascade of four RNA polymerase subunits. s F in the prespore and s E in the mother cell control early stages of development, and are replaced at later stages by s G and s K , respectively. Ultimately, a comprehensive description of the molecular mechanisms underlying spore morphogenesis requires the knowledge of all the intervening genes and their assignment to specific regulons. Here, in an extension of earlier work, DNA macroarrays have been used, and members of the four compartment-specific sporulation regulons have been identified. Genes were identified and grouped based on: i) their temporal expression profile and ii) the use of mutants for each of the four sigma factors and a bofA allele, which allows s K activation in the absence of s G . As a further test, artificial production of active alleles of the sigma factors in non-sporulating cells was employed. A total of 439 genes were found, including previously characterized genes whose transcription is induced during sporulation: 55 in the s F regulon, 154 s E -governed genes, 113 s G -dependent genes, and 132 genes under s K control. The results strengthen the view that the activities of s F , s E , s G and s K are largely compartmentalized, both temporally as well as spatially, and that the major vegetative sigma factor (s A ) is active throughout sporulation. The results provide a dynamic picture of the changes in the overall pattern of gene expression in the two compartments of the sporulating cell, and offer insight into the roles of the prespore and the mother cell at different times of spore morphogenesis.

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Section: Differentiation Of the Compartment-specific Sporulation Regumentioning

confidence: 81%

Section: Differentiation Of the Compartment-specific Sporulation Regumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis

et al. 2005

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“…The first mother cell-specific transcription factor that is induced during sporulation is the alternative s factor s E (Stragier and Losick 1996;Piggot and Losick 2002). The s E regulon has been defined by transcriptional profiling (Eichenberger et al 2003;Feucht et al 2003;Steil et al 2005), and strains harboring deletions in most of the genes in the regulon have been generated (Eichenberger et al 2003). Using the criteria described above, we examined a subset of this ordered library of mutants using the fluorescent membrane fission assay of Sharp and Pogliano (1999).…”

Section: Fisb Is Required For Membrane Fission At the Last Stage Of Ementioning

confidence: 99%

FisB mediates membrane fission during sporulation in Bacillus subtilis

et al. 2013

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How bacteria catalyze membrane fission during growth and differentiation is an outstanding question in prokaryotic cell biology. Here, we describe a protein (FisB, for fission protein B) that mediates membrane fission during the morphological process of spore formation in Bacillus subtilis. Sporulating cells divide asymmetrically, generating a large mother cell and smaller forespore. After division, the mother cell membranes migrate around the forespore in a phagocytic-like process called engulfment. Membrane fission releases the forespore into the mother cell cytoplasm. Cells lacking FisB are severely and specifically impaired in the fission reaction. Moreover, GFP-FisB forms dynamic foci that become immobilized at the site of fission. Purified FisB catalyzes lipid mixing in vitro and is only required in one of the fusing membranes, suggesting that FisB-lipid interactions drive membrane remodeling. Consistent with this idea, the extracytoplasmic domain of FisB binds with remarkable specificity to cardiolipin, a lipid enriched in the engulfing membranes and regions of negative curvature. We propose that membrane topology at the final stage of engulfment and FisB-cardiolipin interactions ensure that the mother cell membranes are severed at the right time and place. The unique properties of FisB set it apart from the known fission machineries in eukaryotes, suggesting that it represents a new class of fission proteins.

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Microbial Development

Fisher¹

2006

Encyclopedia of Molecular Cell Biology and Molecular Medicine

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The σE Regulon and the Identification of Additional Sporulation Genes in Bacillus subtilis

Cited by 214 publications

References 96 publications

Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis

Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis

FisB mediates membrane fission during sporulation in Bacillus subtilis

Microbial Development

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