The Zinc Finger Transcription Factor Fts2 Represses the Yeast-to-Filament Transition in the Dimorphic Yeast Yarrowia lipolytica

Chen, Jia-Wen; Mao, Yi-Sheng; Yan, Lv-Qiao; Gao, Xiang-Dong

doi:10.1128/msphere.00450-22

Cited by 4 publications

(3 citation statements)

References 51 publications

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“…During the Cu(II)-induced dimorphic transition in Y. lipolytica , we could find the participation of many recently reported genes are involved, including YlGPI7 , YlCDC25 , YlRAC1 , YlTEC1 , YlRSR1 , and FTS2 ( Table 3 ) [ 41 ]. These genes should play a common role in the yeast-to-hypha transition of Y. lipolytica .…”

Section: Discussionmentioning

confidence: 99%

Copper Ion Mediates Yeast-to-Hypha Transition in Yarrowia lipolytica

Ran

Zhao

Jiao

et al. 2023

JoF

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Copper is an essential element that maintains yeast physiological function at low concentrations, but is toxic in excess. This study reported that Cu(II) significantly promoted the yeast-to-hypha transition of Yarrowia lipolytica in dose-dependent manner. Strikingly, the intracellular Cu(II) accumulation was drastically reduced upon hyphae formation. Moreover, we investigated the effect of Cu(II) on the physiological function of Y. lipolytica during the dimorphic transition and found that cellular viability and thermomyces lanuginosus lipase (TLL) were both influenced by the Cu(II)-induced yeast-to-hypha transition. Overall, hyphal cells survived better than yeast-form cells with copper ions. Furthermore, transcriptional analysis of the Cu(II)-induced Y. lipolytica before and after hyphae formation revealed a transition state between them. The results showed multiple differentially expressed genes (DEGs) were turned over between the yeast-to-transition and the transition-to-hyphae processes. Furthermore, gene set enrichment analysis (GSEA) identified that multiple KEGG pathways, including signaling, ion transport, carbon and lipid metabolism, ribosomal, and other biological processes, were highly involved in the dimorphic transition. Importantly, overexpression screening of more than thirty DEGs further found four novel genes, which are encoded by YALI1_B07500g, YALI1_C12900g, YALI1_E04033g, and YALI1_F29317g, were essential regulators in Cu-induced dimorphic transition. Overexpression of each of them will turn on the yeast-to-hypha transition without Cu(II) induction. Taken together, these results provide new insight to explore further the regulatory mechanism of dimorphic transition in Y. lipolytica.

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Section: Discussionmentioning

confidence: 99%

Copper Ion Mediates Yeast-to-Hypha Transition in Yarrowia lipolytica

Ran

Zhao

Jiao

et al. 2023

JoF

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“…Several proteins, including transcription factors, Mhy1, Hoy1, Tec1, Rim101, Fts1, Fts2, Nrg1, Tup1, and Ssn6, and regulators, Ras2, Rac1, and Bmh1, are shown to be associated with the transition of cell morphology in Y. lipolytica (Chen et al 2022 ; Hurtado et al 2000 ; Hurtado and Rachubinski 1999 , 2002a , b ; Mao et al 2023 ; Richard et al 2001 ; Shu et al 2021 ; Torres-Guzmán and Domínguez 1997 ; Zhao et al 2013 ). The relationships between these proteins and the LTPs are issues to be elucidated in the future.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Utilization of n-alkane and roles of lipid transfer proteins in Yarrowia lipolytica

Fukuda

2023

World J Microbiol Biotechnol

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Yarrowia lipolytica, a dimorphic yeast belonging to the Ascomycota, has potent abilities to utilize hydrophobic compounds, such as n-alkanes and fatty acids, as carbon and energy sources. Yarrowia lipolytica can synthesize and accumulate large amounts of lipids, making it a promising host to produce various lipids and convert n-alkanes to useful compounds. For advanced use of Y. lipolytica in these applications, it is necessary to understand the metabolism of these hydrophobic compounds in this yeast and the underlying molecular mechanisms. In this review, current knowledge on the n-alkane metabolism and how this is regulated in Y. lipolytica is summarized. Furthermore, recent studies revealed that lipid transfer proteins are involved in the utilization of n-alkanes and the regulation of cell morphology in response to n-alkanes. This review discusses the roles of membrane lipids in these processes in Y. lipolytica.

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“…lipolytica is a dimorphic yeast that changes its cell morphology between the yeast form and pseudohyphal or hyphal form in response to various environmental conditions, including pH and nutrition (34). The cell morphology is regulated by transcription factors, such as Mhy1 and Hoy1, that promote the transition from the yeast to hyphal form and by transcription factors, such as Znc1, Tec1, Fts1, and Nrg1, that promote the transition from the hyphal to yeast form (35)(36)(37)(38)(39)(40). Nonetheless, the entire picture of the transcriptional regulatory network of cell morphogenesis in Y. lipolytica remains to be established.…”

Section: Introductionmentioning

confidence: 99%

Mar1, an HMG-box protein, regulatesn-alkane adsorption and cell morphology of the dimorphic yeastYarrowia lipolytica

Kimura-Ishimaru,

Liang,

Matsuse

et al. 2024

Preprint

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The dimorphic yeastYarrowia lipolyticapossesses an excellent ability to utilizen-alkane as a sole carbon and energy source. Although there are detailed studies on the enzymes that catalyze the reactions in the metabolic processes ofn-alkane inY. lipolytica, the molecular mechanism underlying the incorporation ofn-alkane into the cells remains to be elucidated. BecauseY. lipolyticaadsorbsn-alkane, we postulated thatY. lipolyticaincorporatesn-alkane through direct interaction with it. We isolated and characterized mutants defective in adsorption ton-hexadecane. One of the mutants harbored a nonsense mutation inMAR1(Morphology andn-alkane Adsorption Regulator) encoding a protein containing a high mobility group box. The deletion mutant ofMAR1exhibited defects in adsorption ton-hexadecane and filamentous growth on solid media, whereas the strain that overexpressedMAR1exhibited hyperfilamentous growth. Fluorescence microscopic observations suggested that Mar1 localizes in the nucleus. RNA-seq analysis revealed the alteration of the transcript levels of several genes, including those encoding transcription factors and cell surface proteins, by the deletion ofMAR1. These findings suggest thatMAR1is involved in the transcriptional regulation of the genes required forn-alkane adsorption and cell morphology transition.

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The Zinc Finger Transcription Factor Fts2 Represses the Yeast-to-Filament Transition in the Dimorphic Yeast Yarrowia lipolytica

Abstract: The repressors of filamentation are important negative regulators of the yeast-to-filament transition. However, except in Candida albicans , very few repressors of filamentation are known in dimorphic fungi.

Cited by 4 publications

References 51 publications

Copper Ion Mediates Yeast-to-Hypha Transition in Yarrowia lipolytica

Copper Ion Mediates Yeast-to-Hypha Transition in Yarrowia lipolytica

Utilization of n-alkane and roles of lipid transfer proteins in Yarrowia lipolytica

Mar1, an HMG-box protein, regulatesn-alkane adsorption and cell morphology of the dimorphic yeastYarrowia lipolytica

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