The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphatase

Wicky, Sidonie; Tjandra, Hendri; Schieltz, David; Yates, John R.; Kellogg, Douglas R.

doi:10.1091/mbc.e10-06-0487

Cited by 33 publications

(71 citation statements)

References 83 publications

(141 reference statements)

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“…The proteins Zds1 and Zds2, which associate with and regulate PP2A Cdc55 (Yasutis et al 2010;Wicky et al 2011) help tip the kinase-phosphatase balance in favor of Cdk1 (Queralt and Uhlmann 2008b). They probably accomplish this by binding and retaining PP2A Cdc55 in the cytoplasm, thereby lowering its nuclear concentration (Rossio and Yoshida 2011), but the precise mechanism remains unclear.…”

Section: Fear Pathwaymentioning

confidence: 99%

Mitotic Exit and Separation of Mother and Daughter Cells

2012

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Productive cell proliferation involves efficient and accurate splitting of the dividing cell into two separate entities. This orderly process reflects coordination of diverse cytological events by regulatory systems that drive the cell from mitosis into G1. In the budding yeast Saccharomyces cerevisiae, separation of mother and daughter cells involves coordinated actomyosin ring contraction and septum synthesis, followed by septum destruction. These events occur in precise and rapid sequence once chromosomes are segregated and are linked with spindle organization and mitotic progress by intricate cell cycle control machinery. Additionally, critical parts of the mother/daughter separation process are asymmetric, reflecting a form of fate specification that occurs in every cell division. This chapter describes central events of budding yeast cell separation, as well as the control pathways that integrate them and link them with the cell cycle.

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Section: Fear Pathwaymentioning

confidence: 99%

Mitotic Exit and Separation of Mother and Daughter Cells

2012

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“…108,109 Suppression of this phenotype by loss of Swe1p suggested that the Zds proteins regulate the Swe1pdependent G 2 /M checkpoint. 111 In deciphering the mechanism of this regulation, Yasutis et al, 101 relying on a genetic approach that focused on Zds2p, and Wicky et al, 104 relying on a more biochemical approach that focused on Zds1p, identified Cdc55p, a regulatory subunit of PP2A, as a Zds-interacting protein. 101,104 These data are consistent with high-throughput studies (see refs.…”

Section: G 2 /M Checkpoint In S Pombe and Vertebratesmentioning

confidence: 99%

“…110) that showed protein-protein interactions between the Zds proteins and Cdc55p as well as with reports of co-immunopurification of Cdc55p and Zds1p. 104,112 Two observations position PP2A Cdc55p downstream of the Zds proteins in the G 2 /M checkpoint: (1) accelerated entry into mitosis upon Zds1p or Zds2p overexpression is Cdc55p-dependent, and (2) deletion of CDC55 suppresses the G 2 arrest phenotype (long buds) of zds1Δ zds2Δ cells. 101 These observations indicate that the Zds proteins inhibit Cdc55p at G 2 /M, allowing the transition into mitosis.…”

Section: G 2 /M Checkpoint In S Pombe and Vertebratesmentioning

confidence: 99%

Cell cycle checkpoint regulators reach a zillion

Yasutis

Kozminski

2013

Cell Cycle

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Entry into mitosis is regulated by a checkpoint at the boundary between the G2 and M phases of the cell cycle (G2/M). In many organisms, this checkpoint surveys DNA damage and cell size and is controlled by both the activation of mitotic cyclin-dependent kinases (Cdks) and the inhibition of an opposing phosphatase, protein phosphatase 2A (PP2A). Misregulation of mitotic entry can often lead to oncogenesis or cell death. Recent research has focused on discovering the signaling pathways that feed into the core checkpoint control mechanisms dependent on Cdk and PP2A. Herein, we review the conserved mechanisms of the G2/M transition, including recently discovered upstream signaling pathways that link cell growth and DNA replication to cell cycle progression. Critical consideration of the human, frog and yeast models of mitotic entry frame unresolved and emerging questions in this field, providing a prediction of signaling molecules and pathways yet to be discovered.

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“…4 Among them, the regulatory B subunit is largely responsible for the subcellular localization and substrate specificity of PP2A. 4 In budding yeast, cells lacking Cdc55 (a regulatory B subunit) exhibit multiple cell cycle defects, including mitotic entry, [5][6][7][8][9][10] spindle assembly checkpoint (SAC), 5,[11][12][13] and precocious mitotic exit. 12,[14][15][16][17][18] We have proposed previously that PP2A-Cdc55 activity is largely controlled by its subcellular distribution.…”

Section: Introductionmentioning

confidence: 99%