The yeast ATP synthase subunit 4: structure and function

The enigmatic mitochondrial ORF ymf39 codes for ATP synthase chain b

“…The nuclear-encoded ATP4 sequences from animals share signi®cant sequence similarity with those from fungi over the entire length of the protein, con®rming earlier reports from others (40). Surprisingly, within the region where Ymf39 proteins are most conserved (residue 11±54 in the S.ecuadoriensis protein), the similarity between ATP4 proteins from animals and fungi is only marginal.…”

The enigmatic mitochondrial ORF ymf39 codes for ATP synthase chain b

“…The nuclear-encoded ATP4 sequences from animals share signi®cant sequence similarity with those from fungi over the entire length of the protein, con®rming earlier reports from others (40). Surprisingly, within the region where Ymf39 proteins are most conserved (residue 11±54 in the S.ecuadoriensis protein), the similarity between ATP4 proteins from animals and fungi is only marginal.…”

Mitochondrial Efficiency-Dependent Viability of Saccharomyces cerevisiae Mutants Carrying Individual Electron Transport Chain Component Deletions

“…Both subunit b and d are components of the stator stalk of ATP synthase. Therefore, the absence of these genes could lead to structural instability of the F 1 F 0 complex ( Norais et al, 1991 ; Velours et al, 1989 ).…”

The Mitochondrial F0F1-ATPase Proton Pump Is Required for Function of the Proapoptotic Protein Bax in Yeast and Mammalian Cells

“…ATP4-deficient PVY10 cells were not killed after switching from glucose-to galactose-It was possible that the genetic disruption or pharmacological inhibition of the F0F1-ATPase proton pump indi-containing medium (Figure 2A). In contrast, cells of the isogenic wild-type strain (D273-10B/A) that had been rectly suppressed Bax-mediated lethality in yeast by blocking respiration (Velours et al, 1989;, transformed with pGilda-Bax began to die within 12 hr after switching to galactose.…”

“…mutagenesis (Velours et al, 1989;Paul et al, 1992), thus day to induce expression of Bax, and then plated on glucose-based semisolid medium, which suppresses the GAL1 promoter in pGilda. The plating efficiency of ATP4-deficient PVY10 cells was essentially the same, regardless of whether they contained the pGilda-Bax or pGilda plasmids (Figure 2B).…”

“…tained in DMEM-high glucose 10% serum medium at the nonpermissive temperature of 39ЊC and cultured at either 5 ϫ 10 4 cells/0.1 ml Yeast Methods in 96-well flat bottom plates or at 10 6 cells/5 ml in 6 cm dishes for Yeast strains and plasmids used for these studies have been decell death assays. The medium was then changed with fresh 32ЊC scribed previously (Velours et al, 1989;Paul et al, 1992;Giraud and medium, and cells were cultured at 32ЊC with or without various Velours, 1994;Zha et al, 1996). For generation of Bax-resistant concentrations of oligomycin or antimycin A for 12 hr.…”

The enigmatic mitochondrial ORF ymf39 codes for ATP synthase chain b

“…The nuclear-encoded ATP4 sequences from animals share signi®cant sequence similarity with those from fungi over the entire length of the protein, con®rming earlier reports from others (40). Surprisingly, within the region where Ymf39 proteins are most conserved (residue 11±54 in the S.ecuadoriensis protein), the similarity between ATP4 proteins from animals and fungi is only marginal.…”

Mitochondrial Efficiency-Dependent Viability of Saccharomyces cerevisiae Mutants Carrying Individual Electron Transport Chain Component Deletions

“…Both subunit b and d are components of the stator stalk of ATP synthase. Therefore, the absence of these genes could lead to structural instability of the F 1 F 0 complex ( Norais et al, 1991 ; Velours et al, 1989 ).…”

The Mitochondrial F0F1-ATPase Proton Pump Is Required for Function of the Proapoptotic Protein Bax in Yeast and Mammalian Cells

“…ATP4-deficient PVY10 cells were not killed after switching from glucose-to galactose-It was possible that the genetic disruption or pharmacological inhibition of the F0F1-ATPase proton pump indi-containing medium (Figure 2A). In contrast, cells of the isogenic wild-type strain (D273-10B/A) that had been rectly suppressed Bax-mediated lethality in yeast by blocking respiration (Velours et al, 1989;, transformed with pGilda-Bax began to die within 12 hr after switching to galactose.…”

“…mutagenesis (Velours et al, 1989;Paul et al, 1992), thus day to induce expression of Bax, and then plated on glucose-based semisolid medium, which suppresses the GAL1 promoter in pGilda. The plating efficiency of ATP4-deficient PVY10 cells was essentially the same, regardless of whether they contained the pGilda-Bax or pGilda plasmids (Figure 2B).…”

“…tained in DMEM-high glucose 10% serum medium at the nonpermissive temperature of 39ЊC and cultured at either 5 ϫ 10 4 cells/0.1 ml Yeast Methods in 96-well flat bottom plates or at 10 6 cells/5 ml in 6 cm dishes for Yeast strains and plasmids used for these studies have been decell death assays. The medium was then changed with fresh 32ЊC scribed previously (Velours et al, 1989;Paul et al, 1992;Giraud and medium, and cells were cultured at 32ЊC with or without various Velours, 1994;Zha et al, 1996). For generation of Bax-resistant concentrations of oligomycin or antimycin A for 12 hr.…”

The enigmatic mitochondrial ORF ymf39 codes for ATP synthase chain b

“…The nuclear-encoded ATP4 sequences from animals share signi®cant sequence similarity with those from fungi over the entire length of the protein, con®rming earlier reports from others (40). Surprisingly, within the region where Ymf39 proteins are most conserved (residue 11±54 in the S.ecuadoriensis protein), the similarity between ATP4 proteins from animals and fungi is only marginal.…”

Mitochondrial Efficiency-Dependent Viability of Saccharomyces cerevisiae Mutants Carrying Individual Electron Transport Chain Component Deletions

“…Both subunit b and d are components of the stator stalk of ATP synthase. Therefore, the absence of these genes could lead to structural instability of the F 1 F 0 complex ( Norais et al, 1991 ; Velours et al, 1989 ).…”

The Mitochondrial F0F1-ATPase Proton Pump Is Required for Function of the Proapoptotic Protein Bax in Yeast and Mammalian Cells

“…ATP4-deficient PVY10 cells were not killed after switching from glucose-to galactose-It was possible that the genetic disruption or pharmacological inhibition of the F0F1-ATPase proton pump indi-containing medium (Figure 2A). In contrast, cells of the isogenic wild-type strain (D273-10B/A) that had been rectly suppressed Bax-mediated lethality in yeast by blocking respiration (Velours et al, 1989;, transformed with pGilda-Bax began to die within 12 hr after switching to galactose.…”

“…mutagenesis (Velours et al, 1989;Paul et al, 1992), thus day to induce expression of Bax, and then plated on glucose-based semisolid medium, which suppresses the GAL1 promoter in pGilda. The plating efficiency of ATP4-deficient PVY10 cells was essentially the same, regardless of whether they contained the pGilda-Bax or pGilda plasmids (Figure 2B).…”

“…tained in DMEM-high glucose 10% serum medium at the nonpermissive temperature of 39ЊC and cultured at either 5 ϫ 10 4 cells/0.1 ml Yeast Methods in 96-well flat bottom plates or at 10 6 cells/5 ml in 6 cm dishes for Yeast strains and plasmids used for these studies have been decell death assays. The medium was then changed with fresh 32ЊC scribed previously (Velours et al, 1989;Paul et al, 1992;Giraud and medium, and cells were cultured at 32ЊC with or without various Velours, 1994;Zha et al, 1996). For generation of Bax-resistant concentrations of oligomycin or antimycin A for 12 hr.…”