The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism

Moseley, James B.; Goode, Bruce L.

doi:10.1128/mmbr.00013-06

Cited by 350 publications

(439 citation statements)

References 435 publications

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“…7). The fact that actin within the cell body is needed for cell polarization is consistent with the already appreciated role for actin in this cellular location for cell migration (Cramer et al, 1997;Small et al, 1998;Svitkina et al, 1997;Verkhovsky et al, 1999a) and with the known role for actin-filament cables in the cell body for yeast cell polarity (reviewed by Moseley and Goode, 2006). Although our work shows that oriented actin-filament bundles within the cell body are necessary for cell polarization, it does not address whether they are sufficient, an important direction for future studies.…”

Section: Discussionsupporting

confidence: 49%

ADF/cofilin family proteins control formation of oriented actin-filament bundles in the cell body to trigger fibroblast polarization

Mseka

Bamburg

Cramer

2007

Journal of Cell Science

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How formation of the front and rear of a cell are coordinated during cell polarization in migrating cells is not well understood. Time-lapse microscopy of live primary chick embryo heart fibroblasts expressing GFP-actin show that, prior to cell polarization, polymerized actin in the cell body reorganizes to form oriented actin-filament bundles spanning the entire cell body. Within an average of 5 minutes of oriented actin bundles forming, localized cell-edge retraction initiates at either the side or at one end of the newly formed bundles and then elaborates around the nearest end of the bundles to form the cell rear, the first visual break in cell symmetry. Localized net protrusion occurs at the opposing end of the bundles to form the cell front and lags formation of the rear of the cell. Consequently, cells acquire full polarity and start to migrate in the direction of the long axis of the bundles, as previously documented for already migrating cells. When ADF/cofilin family protein activity or actin-filament disassembly is specifically blocked during cell polarization, reorganization of polymerized actin to form oriented actin-filament bundles in the cell body fails, and formation of the cell rear and front is inhibited. We conclude that formation of oriented actin-filament bundles in the cell body requires ADF/cofilin family proteins, and is an early event needed to coordinate the spatial location of the cell rear and front during fibroblast polarization.

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Section: Discussionsupporting

confidence: 49%

ADF/cofilin family proteins control formation of oriented actin-filament bundles in the cell body to trigger fibroblast polarization

Mseka

Bamburg

Cramer

2007

Journal of Cell Science

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“…Finally, spots 17 and 18 resulted in the identification of actin, and spots 20 and 21 were assigned to mannose-1-phosphate guanyltransferase; both proteins are required for normal cell wall structure. In fact, actin is a structural protein involved in many cytoskeletal functions such as cell wall organization and biogenesis [33,34] whilst mannose-1-phosphate guanyltransferase is an enzyme responsible for cell wall biosynthesis [35].…”

Section: Resultsmentioning

confidence: 99%

Different carbon sources affect lifespan and protein redox state during Saccharomyces cerevisiae chronological ageing

Magherini

Carpentieri

Amoresano

et al. 2009

Cell. Mol. Life Sci.

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Abstract. In this study, a proteomic approach that combines selective labelling of proteins containing reduced cysteine residues with two-dimensional electrophoresis/mass spectrometry was used to evaluate the redox state of protein cysteines during chronological ageing in Saccharomyces cerevisiae. The procedure was developed on the grounds that biotinconjugated iodoacetamide (BIAM) specifically reacts with reduced cysteine residues. BIAM-labelled proteins can then be selectively isolated by streptavidin affinity capture. We compared cells grown on 2 % glucose in the exponential phase and during chronological ageing and we found that many proteins undergo cysteine oxidation. The target proteins include enzymes involved in glucose metabolism. Both caloric restriction and growth on glycerol resulted in a decrease in the oxidative modification. Furthermore, in these conditions a reduced production of ROS and a more negative glutathione half cell redox potential were observed.

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“…Actin1 is one of the most important structural proteins involved in such critical processes as endocytosis, cytokinesis, cell polarity, and morphogenesis (22). Because cell morphology of several translocant cells was different from the parental SanI cells (see Fig.…”

Section: Actin1 Protein Was Modified In Several Translocantsmentioning

confidence: 99%

Cellular and molecular effects of nonreciprocal chromosome translocations in Saccharomyces cerevisiae

Nikitin

Tosato²,

Zavec³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Saccharomyces cerevisiae strains harboring a nonreciprocal, bridgeinduced translocation (BIT) between chromosomes VIII and XV exhibited an abnormal phenotype comprising elongated buds and multibudded, unevenly nucleated pseudohyphae. In these cells, we found evidence of molecular effects elicited by the translocation event and specific for its particular genomic location. Expression of genes flanking both translocation breakpoints increased up to five times, correlating with an increased RNA polymerase II binding to their promoters and with their histone acetylation pattern. Microarray data, CHEF, and quantitative PCR confirmed the data on the dosage of genes present on the chromosomal regions involved in the translocation, indicating that telomeric fragments were either duplicated or integrated mostly on chromosome XI. FACS analysis revealed that the majority of translocant cells were blocked in G1 phase and a few of them in G2. Some cells showed a posttranslational decrease of cyclin B1, in agreement with elongated buds diagnostic of a G2/M phase arrest. The actin1 protein was in some cases modified, possibly explaining the abnormal morphology of the cells. Together with the decrease in Rad53p and the lack of its phosphorylation, these results indicate that these cells have undergone adaptation after checkpointmediated G2/M arrest after chromosome translocation. These BIT translocants could serve as model systems to understand further the cellular and molecular effects of chromosome translocation and provide fundamental information on its etiology of neoplastic transformation in mammals.DNA integration ͉ yeast ͉ genome expression ͉ genome dynamics ͉ transcription

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The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism

Cited by 350 publications

References 435 publications

ADF/cofilin family proteins control formation of oriented actin-filament bundles in the cell body to trigger fibroblast polarization

ADF/cofilin family proteins control formation of oriented actin-filament bundles in the cell body to trigger fibroblast polarization

Different carbon sources affect lifespan and protein redox state during Saccharomyces cerevisiae chronological ageing

Cellular and molecular effects of nonreciprocal chromosome translocations in Saccharomyces cerevisiae

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