The WUSCHEL-related homeobox transcription factor MtWOX9-1 stimulates somatic embryogenesis in Medicago truncatula

Tvorogova, Varvara E.; Fedorova, Yulia; Potsenkovskaya, Elina A.; Kudriashov, Andrei; Efremova, Elena P.; Kvitkovskaya, Valeria A.; Wolabu, Tezera W.; Zhang, Fei; Tadege, Million; Лутова, Л. А.

doi:10.1007/s11240-019-01648-w

Cited by 41 publications

(50 citation statements)

References 64 publications

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“…As well as w9o calli appear to be more embryogenic than wt ones according to our data (Tvorogova et al, 2019), we supposed that MtWOX9-1 should specifically stimulate expression of genes related with embryo development and development of pods. To check this hypothesis, we selected the genes with specific expression in seedpod (expression level in seedpod is at least 4 times higher than average expression level measured in seedpod, leafblade, nodule, root, flower and bud) from the M. truncatula genome database (Krishnakumar et al, 2015).…”

Section: Resultssupporting

confidence: 56%

“…It is important to note that in this study we used a single line with MtWOX9-1 overexpression, therefore one cannot exclude the possibility that observed effects are related with specific location of transgenic insert in this line. However, according to our results (Tvorogova et al, 2019), the positive effect of MtWOX9-1 overexpression on embryogenic capacity was observed after several independent transformation events, therefore we can assume that the majority of the effects demonstrated in this study are not unique for specific transgenic line.…”

Section: Discussionsupporting

confidence: 48%

“…The observed differences between w9o and wt calli and control can be considered as the specific effect of MtWOX9-1 overexpression but, on the other hand, they may result from SE process itself, which tends to start earlier in w9o calli (Tvorogova et al, 2019). Thus, these data may be useful both for MtWOX9-1 target search and for the search for new SEassociated genes and SE stimulators.…”

Section: Resultsmentioning

confidence: 97%

“…In our previous studies, three new M. truncatula genes of the WOX family, that are expressed during SE, were found: MtWOX9-1, MtWOX11-like and STENOFOLIA (MtWOX1) (Tvorogova et al, 2015). It was further shown that overexpression of STF or MtWOX9-1 stimulates the emergence of somatic embryos (Tvorogova et al, 2016(Tvorogova et al, , 2019. In the present work, we concentrated on studying the functions of the MtWOX9-1 gene, analyzing how its overexpression affects the expression profile of embryogenic callus.…”

Section: Introductionmentioning

confidence: 94%

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Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression

et al. 2019

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Somatic embryogenesis (SE) is the development of embryo-like structures from somatic plant tissues. This process rarely can be observed in nature, but for many plant species, in vitro protocols are developed, which allow to obtain somatic embryos formation directly from tissues of plant explant or from the embryogenic callus. SE is widely used for plant propagation and transformation; therefore, the search for SE stimulators and revealing of the mechanisms of their functioning are very important for biotechnology. Among the SE regulators, proteins of the WOX family play significant roles. WOX (WUSCHEL-RELATED HOMEOBOX) is a homeodomain-containing transcription factor family. Different WOX genes function in different plant organs and tissues, maintaining meristem activity and regulating cell proliferation and differentiation. Recently, we have shown that transcription factor MtWOX9-1, belonging to the WOX family, can stimulate SE in the Medicago truncatula callus culture. In this research, transcriptomic analysis of highly embryogenic calli with MtWOX9-1 overexpression was performed in comparison to wildtype calli. It was shown that MtWOX9-1 overexpression led to the activation of several groups of genes, including genes related to cell division, tissue diff erentiation, and seed development. Enriched GO pathways included several groups related to histone methyltransferase activity as well as DNA methylation and chromatin binding, suggesting major epigenetic changes that occur in call overexpressing MtWOX9-1. Using Medicago Truncatula Gene Expression Atlas, we also iden tified a group of genes coding for transcription factors that were both coexpressed with MtWOX9-1 in different plant organs and differentially expressed in our samples. These genes are putative targets of MtWOX9-1, and they may act in the same pathway with this regulator during SE.

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Section: Resultssupporting

confidence: 56%

Section: Discussionsupporting

confidence: 48%

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 94%

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Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression

et al. 2019

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“…In order to stimulate the expression of PIN1 and to control early zygotic embryo patterning, ARF5 cooperates with the WUSCHEL-RELATED HOMEOBOX (WOX) TF gene family members, including WOX1, WOX2, WOX5, WOX8 and WOX9 [105][106][107]. Although the expression of the WOX2, WOX8 and WOX9 genes was reported in an embryogenic culture of several plants, including grapevine, spruce, Arabidopsis, cotton, barrelclover, larch and longan [64,75,[108][109][110][111][112][113][114], the auxin-signalling regulators that control the WOX genes in SE induction have not been studied as yet. In particular, WOX2 seems to be an interesting ARF5 target candidate in regulating SE because of its role in balancing the cytokinin versus the auxin hormone pathways during the establishment of the shoot identity in the zygotic embryo [115].…”

Section: The Core Regulatory Components Of the Auxin-signalling Pathwmentioning

confidence: 99%

Current Perspectives on the Auxin-Mediated Genetic Network that Controls the Induction of Somatic Embryogenesis in Plants

Wójcik

Wójcikowska

Gaj

2020

IJMS

109

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Auxin contributes to almost every aspect of plant development and metabolism as well as the transport and signalling of auxin-shaped plant growth and morphogenesis in response to endo- and exogenous signals including stress conditions. Consistently with the common belief that auxin is a central trigger of developmental changes in plants, the auxin treatment of explants was reported to be an indispensable inducer of somatic embryogenesis (SE) in a large number of plant species. Treating in vitro-cultured tissue with auxins (primarily 2,4-dichlorophenoxyacetic acid, which is a synthetic auxin-like plant growth regulator) results in the extensive reprogramming of the somatic cell transcriptome, which involves the modulation of numerous SE-associated transcription factor genes (TFs). A number of SE-modulated TFs that control auxin metabolism and signalling have been identified, and conversely, the regulators of the auxin-signalling pathway seem to control the SE-involved TFs. In turn, the different expression of the genes encoding the core components of the auxin-signalling pathway, the AUXIN/INDOLE-3-ACETIC ACIDs (Aux/IAAs) and AUXIN RESPONSE FACTORs (ARFs), was demonstrated to accompany SE induction. Thus, the extensive crosstalk between the hormones, in particular, auxin and the TFs, was revealed to play a central role in the SE-regulatory network. Accordingly, LEAFY COTYLEDON (LEC1 and LEC2), BABY BOOM (BBM), AGAMOUS-LIKE15 (AGL15) and WUSCHEL (WUS) were found to constitute the central part of the complex regulatory network that directs the somatic plant cell towards embryogenic development in response to auxin. The revealing picture shows a high degree of complexity of the regulatory relationships between the TFs of the SE-regulatory network, which involve direct and indirect interactions and regulatory feedback loops. This review examines the recent advances in studies on the auxin-controlled genetic network, which is involved in the mechanism of SE induction and focuses on the complex regulatory relationships between the down- and up-stream targets of the SE-regulatory TFs. In particular, the outcomes from investigations on Arabidopsis, which became a model plant in research on genetic control of SE, are presented.

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WUSCHEL: a master regulator in plant growth signaling

2020

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The WUSCHEL-related homeobox transcription factor MtWOX9-1 stimulates somatic embryogenesis in Medicago truncatula

Cited by 41 publications

References 64 publications

Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression

Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression

Current Perspectives on the Auxin-Mediated Genetic Network that Controls the Induction of Somatic Embryogenesis in Plants

WUSCHEL: a master regulator in plant growth signaling

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