The Wound Healing Effect of Hydnocarpi Semen Extract on Ulcer in Diabetic Mice

Abstract

-The extract from Hydnocarpi Semen (HS) has been used to treat leprosy and its anti-inflammatory activity has been reported. However, the effect of HS on the treatment of diabetic or peripheral ulcer is not well known. We therefore examined its wound healing effects on ulcer area in diabetic mice. GC and GC/MS analysis with the total extract of HS show that the main constituents of the extract are chaulmoogric acid, hydnocarpic acid, and gorlic acid. Whereas HS showed wound healing effect in diabetic ulcer, there was no hypoglycemic effect in diabetic mice. The treatment of HS extract significantly decreased the level of total WBC and neutrophils in mice compared to control mice. Cutting ulcer was induced by the round-shaped punch on the backside of diabetic mice and the extract of HS was given orally or topically. The wound area score significantly decreased after treatment of HS at dose of 50 mg/kg. The treatment of HS also induced the activation of macrophages and increased the production of IL-12 and TNF-α in macrophages, indicating that the wound healing by HS extract is associated with the inflammatory effect via the activation of macrophages. Our results suggest that HS extract can be a new therapeutic candidate for treatment of diabetic ulcer.

Then-Hexane, ethylacetate, and butanol fractions from Hydnocarpi Semen enhanced wound healing in a mice ulcer model

“…healing effect in hyperglycemic mice. (10) However, it is unknown which constituents of HS extract show the wound healing. Therefore, this study focused on the wound healing by n-Hexane, EtOAc, and BuOH fractions from HS crude extract in the skin ulcer in diabetic mice.…”

“…The area of wound healing was determined to demonstrate the antiulcer effect of three fractions (n-hexane, EtOAc, and BuOH) on ulcer in hyperglycemic mice. (10) Treatment with the fractions by oral administration increased wound healing in ulcer lesion compared to non-treated control and the wound area score was significantly decreased at a dose-dependent manner. Especially, BuOH fraction exhibited the most potent wound healing effect and the wound area score significantly decreased after treatment of BuOH fraction even at dose of 2 mg/kg (Figure 2) suggesting that BuOH fraction may have major components for wound healing.…”

“…The wound surface area was checked by tracing the boundary of open wound on semi-transparent paper and calculation of area was measured by using a graph paper. (10) …”

“…Diabetic ulcer was induced as previously described. (10) The ulcerated mice were treated by oral administration with the n-hexane, EtOAc, and BuOH fractions. The wound surface area was checked by tracing the boundary of open wound on semi-transparent paper and calculation of area was measured by using a graph paper.…”

“…(6)(7)(8)(9) We, previously, demonstrated that the extract of HS showed the wound healing effect on ulcer in diabetic mice. (10) However, the component showing wound healing has not been elucidated yet. In this study, fractions of n-Hexane, ethylacetate (EtOAc), and butanol (BuOH) from HS crude extract were evaluated for their wound healing activity by using in vivo diabetic ulcer…”