The WHO Collaborating Centre for Research and Control of Schistosomiasis at Niamey, Niger

Chippaux, Jean‐Philippe; Boulanger, Denis; Brémond, Philippe; Campagne, G.; Véra, C.; Sellin, Bertrand

doi:10.1590/s0074-02761997000500030

Cited by 5 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[45] Since these schistosome species often infect snails inhabiting the same bodies of water, examining cercariae or infected snails for estimating the transmission of S. haematobium is always confounded by the need to differentially identify S. haematobium . [678] Although cercariae belonging to these related species are not readily distinguishable morphologically from others within the group, quite a number of approaches have been taken for differential identification. The standard method being infection of laboratory animals and subsequent parasite species identification based on the morphology of the adult worms, but this method is laborious and time consuming.…”

Section: Introductionmentioning

confidence: 99%

Differentiating Schistosoma haematobium from Schistosoma magrebowiei and other closely related schistosomes by polymerase chain reaction amplification of a species specific mitochondrial gene

et al. 2014

View full text Add to dashboard Cite

Introduction:Schistosoma haematobium infection afflicts about 150 million people in 53 countries in Africa and the Middle East. In many endemic areas, S. haematobium is sympatric with Schistosoma bovis, Schistosoma mattheei, Schistosoma curassoni, Schistosoma intercalatum and Schistosoma magrebowiei, its closely related species. In addition, they also develop in the same intermediate snail hosts. Since these schistosome species often infect snails inhabiting the same bodies of water, examining cercariae or infected snails for estimating transmission of S. haematobium is always confounded by the need to differentially identify S. haematobium from these other species. Recently, differentiating S. haematobium by polymerase chain reaction (PCR) from S. bovis, S. mattheei, S. curassoni and S. intercalatum, but not from S. magrebowiei was reported. However, to be able to evaluate residual S. haematobium transmission after control interventions in areas where S. haematobium may be sympatric with S. magrebowiei, a differential tool for accurate monitoring of infected snails is needed.Materials and Methods:Thus in this study, we developed a new PCR assay using a pair of primers, ShND-1/ShND-2, to amplify a target sequence of 1117 bp (GenBank accession number KF834975) from S. haematobium mitochondrion complete genome (GenBank accession number DQ157222). Sensitivity of the assay was determined by PCR amplification of different concentrations of S. haematobium gDNA serially diluted from 10ng to 0.1pg. For assay specificity, different concentrations of gDNA from S. haematobium and the other schistosome species, 20 positive urine samples and five controls as well as 20 infected snails were subjected to PCR amplification, while some of the PCR products were sequenced.Results:The assay detected up to 1pg of S. haematobium gDNA, while a differential identification of S. haematobium DNA content from other closely related species was achieved when applied to urine and naturally infected snails. When a protein-protein blast search was carried out using Blastp, the amplified sequence was found to encode a protein that shows a 100% similarity with S. haematobium nicotinamide adenine dinucleotide dehydrogenase subunit 3 (GenBank accession number YP_626524.1).Conclusion:The PCR assay was sensitive, specific and was able to successfully differentiate S. haematobium from S. magrebowiei, in addition to its other closely related animal infective schistosome species.

show abstract

Section: Introductionmentioning

confidence: 99%

Differentiating Schistosoma haematobium from Schistosoma magrebowiei and other closely related schistosomes by polymerase chain reaction amplification of a species specific mitochondrial gene

et al. 2014

View full text Add to dashboard Cite

show abstract

“…2,3 Because S. haematobium and its related schistosome species often infect snails inhabiting the same bodies of water, the assessment of the transmission of S. haematobium by examining cercariae or infected snails is confounded by the need to differentially identify S. haematobium. [4][5][6] Of the schistosome species related to S. haematobium, S. bovis is the most widespread, with reported distribution in the Middle East, the Mediterranean basin, and most parts of Africa. 7,8 The other species of schistosomes examined in this study, S. mattheei, S. margrebowiei, and S. curassoni, are also present in various parts of Africa, although less prevalent.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Schistosoma Haematobium From Related Schistosomes by PCR Amplifying an Inter-Repeat Sequence

Abbasi¹,

King²,

Sturrock³

et al. 2007

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abbasi, I; King, CH; Sturrock, RF; Kariuki, C; Muchiri, E; Hamburger, J; (2007) Abstract. Schistosoma haematobium infects nearly 150 million people, primarily in Africa, and is transmitted by select species of local bulinid snails. These snails can host other related trematode species as well, so that effective detection and monitoring of snails infected with S. haematobium requires a successful differentiation between S. haematobium and any closely related schistosome species. To enable differential detection of S. haematobium DNA by simple polymerase chain reaction (PCR), we designed and tested primer pairs from numerous newly identified Schistosoma DNA repeat sequences. However, all pairs tested were found unsuitable for this purpose. Differentiation of S. haematobium from S. bovis, S. mattheei, S. curassoni, and S. intercalatum (but not from S. margrebowiei) was ultimately accomplished by PCR using one primer from a newly identified repeat, Sh110, and a second primer from a known schistosomal splice-leader sequence. For evaluation of residual S. haematobium transmission after control interventions, this differentiation tool will enable accurate monitoring of infected snails in areas where S. haematobium is sympatric with the most prevalent other schistosome species.

show abstract

Efficiency of the oral, intramuscular and subcutaneous routes for the experimental infection of hamster and sheep with Schistosoma bovis

Oleaga

Ramajo

2004

Veterinary Parasitology

View full text Add to dashboard Cite

The WHO Collaborating Centre for Research and Control of Schistosomiasis at Niamey, Niger

Cited by 5 publications

References 7 publications

Differentiating Schistosoma haematobium from Schistosoma magrebowiei and other closely related schistosomes by polymerase chain reaction amplification of a species specific mitochondrial gene

Differentiating Schistosoma haematobium from Schistosoma magrebowiei and other closely related schistosomes by polymerase chain reaction amplification of a species specific mitochondrial gene

Differentiation of Schistosoma Haematobium From Related Schistosomes by PCR Amplifying an Inter-Repeat Sequence

Efficiency of the oral, intramuscular and subcutaneous routes for the experimental infection of hamster and sheep with Schistosoma bovis

Contact Info

Product

Resources

About