The villi contribute to the mechanics in the guinea pig small intestine

Chen, Xudong; Zhao, Jingbo; Gregersen, Hans

doi:10.1016/j.jbiomech.2007.11.007

Cited by 14 publications

(10 citation statements)

References 26 publications

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“…In the M group, the villus height of duodenum increased while no change was observed for the other parameters. However, the expected increase in the opening angle and inner residual strain was not observed as previously reported (Chen et al, 2008). This may be due to other structural changes.…”

Section: Article In Presssupporting

confidence: 40%

Intestinal remodelling in mink fed with reduced protein content

Chen

Zhao

Nielsen

et al. 2009

Journal of Biomechanics

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Section: Article In Presssupporting

confidence: 40%

Intestinal remodelling in mink fed with reduced protein content

Chen

Zhao

Nielsen

et al. 2009

Journal of Biomechanics

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“…In addition to having motility, the upper GI tract is populated by finger‐like villi of up to a millimeter in length. These villi affect intestinal fluid dynamics, pressure and wall stiffness (Chen et al, ) in a way that has thus far not been replicated in current in vitro models. Our early studies have shown that simply by recreating the topograpy of the small intestine with the same shape, size, and distribution of human intestinal villi, Caco‐2 monolayers present differentiation morphology along the crypt‐villus axis and TEER values more highly correlated with that of in vivo intestines than a 2D (flat) model (Yu et al, ).…”

Section: Introductionmentioning

confidence: 99%

Synthetic small intestinal scaffolds for improved studies of intestinal differentiation

Costello

Jia

Shaffiey

et al. 2014

Biotech & Bioengineering

122

138

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In vitro intestinal models can provide new insights into small intestinal function, including cellular growth and proliferation mechanisms, drug absorption capabilities, and host-microbial interactions. These models are typically formed with cells cultured on 2D scaffolds or transwell inserts, but it is widely understood that epithelial cells cultured in 3D environments exhibit different phenotypes that are more reflective of native tissue. Our focus was to develop a porous, synthetic 3D tissue scaffold with villous features that could support the culture of epithelial cell types to mimic the natural microenvironment of the small intestine. We demonstrated that our scaffold could support the co-culture of Caco-2 cells with a mucus-producing cell line, HT29-MTX, as well as small intestinal crypts from mice for extended periods. By recreating the surface topography with accurately sized intestinal villi, we enable cellular differentiation along the villous axis in a similar manner to native intestines. In addition, we show that the biochemical microenvironments of the intestine can be further simulated via a combination of apical and basolateral feeding of intestinal cell types cultured on the 3D models.

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“…Hence, finite deformation computations were required. Since the villi do not support circumferential tensile stress, the villi height is subtracted from the total thickness for the intestinal stress and strain calculation.…”

Section: Methodsmentioning

confidence: 99%

Stress–strain analysis of duodenal contractility in response to flow and ramp distension in rabbits fed low‐fiber diet

Liu

Zhao

Liao

et al. 2018

Neurogastroenterology Motil

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Background Previously we demonstrated that low‐fiber diet in rabbits affects the passive mechanomorphological properties in the small intestine, resulting in reduced intestinal wall thickness and collagen content, as well as intestinal wall softening. The aim of the present study was to evaluate the contractility in rabbits on long‐term low‐fiber diet and specifically to compare the contraction threshold, the frequency, and the amplitude of flow‐induced and distension‐induced contractions in the duodenum between rabbits on normal diet and on long‐term low‐fiber diet. Methods Ten rabbits were fed a low‐fiber diet for 5 months (Intervention group), and five rabbits were fed normal diet (Control group). The duodenal segments were used for determination of mechanical parameters for analyses of contractility. The duodenal experiments were carried out in organ baths containing physiological Krebs solution. Pressure and diameter changes induced by contractions in response to flow and ramp distension were measured. The frequencies and amplitude of contractions were analyzed. Distension‐induced contraction thresholds and maximum contraction amplitude of flow‐induced contractions were calculated in terms of mechanical stress and strain. Multiple linear regression analyses were applied to study dependencies between contractility parameters and wall thickness, wall area, and muscle layer thickness. Key results During distension, the pressure, stress, and strain thresholds for induction of phasic contraction were biggest in the Intervention Group (P < 0.05). In addition, the contraction frequencies during flow‐induced contraction were highest in the Intervention Group (P < 0.05), whereas the maximum contraction amplitudes in terms of pressure, diameter, stress, and strain were lowest in the Intervention Group (P < 0.05). The contraction thresholds and contraction frequencies were negatively associated with the wall thickness, wall area, and muscle layer thickness, whereas maximum contraction amplitudes were positively associated with the wall thickness, wall area, and muscle layer thickness. Conclusions and inferences Duodenal contractility in rabbits fed with long‐term low‐fiber diet exhibited low contraction amplitudes and high contraction thresholds and frequencies. The changes were associated with the low‐fiber diet‐induced histomorphological remodeling. Studies on detailed structural and functional diet‐induced changes in smooth muscle and intestinal nerves are needed for better understanding the remodeling mechanisms.

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The villi contribute to the mechanics in the guinea pig small intestine

Cited by 14 publications

References 26 publications

Intestinal remodelling in mink fed with reduced protein content

Intestinal remodelling in mink fed with reduced protein content

Synthetic small intestinal scaffolds for improved studies of intestinal differentiation

Stress–strain analysis of duodenal contractility in response to flow and ramp distension in rabbits fed low‐fiber diet

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