Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.01.015 View full text
John R. Griffiths, Simon Perkins, Yvonne Connolly, Lu Zhang, Mark Holland, Valeria Barattini, Luisa Pereira, Anthony Edge, Harald Ritchie, Duncan L. Smith

Abstract: We present the first investigation into the utility of porous graphitic carbon (PGC) as a stationary phase in proteomic workflows involving complex samples. PGC offers chemical and physical robustness and is capable of withstanding extremes of pH and higher temperatures than traditional stationary phases, without the likelihood of catastrophic failure. In addition, unlike separations driven by ion exchange mechanisms, there is no requirement for high levels of non-volatile salts such as potassium chloride in t…

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