The utilisation of CytB and COI barcodes for the identification of bloodmeals and Culicoides species (Diptera: Ceratopogonidae) reveals a variety of novel wildlife hosts in South Africa.

Snyman, Jumari; Snyman, Louwrens Pieter; Labuschagne, Karien; Venter, Gert J.; Venter, Marietjie

doi:10.1016/j.actatropica.2021.105913

Cited by 8 publications

(14 citation statements)

References 43 publications

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“…Bluetongue and African horse sickness virus (AHSV) are also endemic to these regions. Culicoides midges were collected monthly from September 2012 to May 2017, at six sites in the three provinces, for two consecutive nights, using 220 V Onderstepoort light traps, as previously described [ 5 , 16 ]. Subsamples (10% of pooled sample) from various collections were morphologically identified to the species level to establish species composition at the surveillance sites ( Table S1 ) [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

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An Investigation of Culicoides (Diptera: Ceratopogonidae) as Potential Vectors of Medically and Veterinary Important Arboviruses in South Africa

Snyman

Venter

2021

Viruses

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Culicoides-borne viruses such as bluetongue, African horse sickness, and Schmallenberg virus cause major economic burdens due to animal outbreaks in Africa and their emergence in Europe and Asia. However, little is known about the role of Culicoides as vectors for zoonotic arboviruses. In this study, we identify both veterinary and zoonotic arboviruses in pools of Culicoides biting midges in South Africa, during 2012–2017. Midges were collected at six surveillance sites in three provinces and screened for Alphavirs, Flavivirus, Orthobunyavirus, and Phlebovirus genera; equine encephalosis virus (EEV); and Rhaboviridae, by reverse transcription polymerase chain reaction. In total, 66/331 (minimum infection rate (MIR) = 0.4) pools tested positive for one or more arbovirus. Orthobunyaviruses, including Shuni virus (MIR = 0.1) and EEV (MIR = 0.2) were more readily detected, while only 2/66 (MIR = 0.1) Middelburg virus and 4/66 unknown Rhabdoviridae viruses (MIR = 0.0) were detected. This study suggests Culicoides as potential vectors of both veterinary and zoonotic arboviruses detected in disease outbreaks in Africa, which may contribute to the emergence of these viruses to new regions.

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Section: Methodsmentioning

confidence: 99%

“…To date, over 50 arboviruses have been isolated from Culicoides , 45% of which have not been detected in other arthropods [ 1 ]. A recent study demonstrated human feeding to be as high as 11% in field collected Culicoides species, and as yet, little information exists on the role of Culicoides as vectors for zoonotic pathogens [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

An Investigation of Culicoides (Diptera: Ceratopogonidae) as Potential Vectors of Medically and Veterinary Important Arboviruses in South Africa

Snyman

Venter

2021

Viruses

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“…Blood meal sources of disease vectors indicate the host preference (Martínez-de la Puente et al, 2015;Snyman et al, 2021;Videvall et al, 2013), probable reservoir or cycling hosts and also contribute towards vector control and disease distribution (Slama et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Generally, feeding preferences of Culicoides have been classified as a mammalophilic, ornithophilic or opportunistic (Martínez-de la Puente et al, 2015;Riddin et al, 2019) and host selection is the key determinant of the intensity at which pathogens are transmitted (Snyman et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Blood meal sources of disease vectors indicate the host preference (Martínez‐de la Puente et al, 2015; Snyman et al, 2021; Videvall et al, 2013), probable reservoir or cycling hosts and also contribute towards vector control and disease distribution (Slama et al, 2015). Generally, feeding preferences of Culicoides have been classified as a mammalophilic, ornithophilic or opportunistic (Martínez‐de la Puente et al, 2015; Riddin et al, 2019) and host selection is the key determinant of the intensity at which pathogens are transmitted (Snyman et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Blood meal analysis of Culicoides species associated with livestock in West Bengal, India

Kar

Mondal

Pal

et al. 2022

Medical Vet Entomology

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Knowledge gaps exist on the feeding pattern and host range of bluetongue virus vectors, Culicoides species, associated with livestock in India. Adult midges were trapped with ultraviolet light traps at 13 household farms adjacent to human biotope. Host DNA was isolated from individual females (n = 101; blood engorged‐82, gravid‐4 and parous‐15) and subjected to PCR amplification targeting CytB and 16S rRNA gene fragments followed by sequencing of amplified DNA samples. However, DNA sequences from only 71 individuals (70.3%) comprising of 10 Culicoides species were obtained. Blood meal analysis revealed at least 10 species that fed on five mammalian hosts including humans, but surprisingly none tested positive for birds. Results revealed that Culicoides innoxius tested positive for four not previously recognized species indicating a potential role as a vector species. Likewise, Culicoides shortti and Culicoides hegneri preferred goat and cattle respectively as hosts, whereas Culicoides palpifer preferred cattle along with buffalo as hosts, which is being reported for the first time. This is the first document on DNA‐based blood meal identification and feeding preference of Culicoides midges associated with livestock in India.

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Evaluating the morphological and molecular challenges in identifying the afrotropical Atylotus species (Diptera: Tabanidae)

et al. 2022

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The utilisation of CytB and COI barcodes for the identification of bloodmeals and Culicoides species (Diptera: Ceratopogonidae) reveals a variety of novel wildlife hosts in South Africa.

Cited by 8 publications

References 43 publications

An Investigation of Culicoides (Diptera: Ceratopogonidae) as Potential Vectors of Medically and Veterinary Important Arboviruses in South Africa

An Investigation of Culicoides (Diptera: Ceratopogonidae) as Potential Vectors of Medically and Veterinary Important Arboviruses in South Africa

Blood meal analysis of Culicoides species associated with livestock in West Bengal, India

Evaluating the morphological and molecular challenges in identifying the afrotropical Atylotus species (Diptera: Tabanidae)

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