The Use of Tissue-Engineered Skin (Apligraf) to Treat a Newborn With Epidermolysis Bullosa

Falabella, Anna; Schachner, Lawrence A.; Valencia, Isabel C.; Eaglstein, William H.

doi:10.1001/archderm.135.10.1219

Cited by 80 publications

(51 citation statements)

References 22 publications

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“…1,2 One critical obstacle in this promising field is vascularization of the engineered tissues. The most successful applications of tissue engineering to date have been limited to thin (<2 mm) avascular tissues, such as skin 3 and cartilage, 4 in which delivery of nutrients and oxygen occurs by diffusion. Before the establishment of blood flow, cells in the inner portion of an implanted tissue are exposed to an environment with minimal oxygen and nutrients, which can lead to significant cell death.…”

Section: Introductionmentioning

confidence: 99%

Rapid Anastomosis of Endothelial Progenitor Cell–Derived Vessels with Host Vasculature Is Promoted by a High Density of Cotransplanted Fibroblasts

Chen

Aledia

Popson

et al. 2010

Tissue Engineering Part A

183

193

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To ensure survival of engineered implantable tissues thicker than approximately 2-3 mm, convection of nutrients and waste products to enhance the rate of transport will be required. Creating a network of vessels in vitro, before implantation (prevascularization), is one potential strategy to achieve this aim. In this study, we developed three-dimensional engineered vessel networks in vitro by coculture of endothelial cells (ECs) and fibroblasts in a fibrin gel for 7 days. Vessels formed by cord blood endothelial progenitor cell-derived ECs (EPC-ECs) in the presence of a high density of fibroblasts created an interconnected tubular network within 4 days, compared with 5-7 days in the presence of a low density of fibroblasts. Vessels derived from human umbilical vein ECs (HUVECs) in vitro showed similar kinetics. Implantation of the prevascularized tissues into immunecompromised mice, however, revealed a dramatic difference in the ability of EPC-ECs and HUVECs to form anastomoses with the host vasculature. Vascular beds derived from EPC-ECs were perfused within 1 day of implantation, whereas no HUVEC vessels were perfused at day 1. Further, while almost 90% of EPC-EC-derived vascular beds were perfused at day 3, only one-third of HUVEC-derived vascular beds were perfused. In both cases, a high density of fibroblasts accelerated anastomosis by 2-3 days. We conclude that both EPC-ECs and a high density of fibroblasts significantly accelerate the rate of functional anastomosis, and that prevascularizing an engineered tissue may be an effective strategy to enhance convective transport of nutrients in vivo.

show abstract

Section: Introductionmentioning

confidence: 99%

Rapid Anastomosis of Endothelial Progenitor Cell–Derived Vessels with Host Vasculature Is Promoted by a High Density of Cotransplanted Fibroblasts

Chen

Aledia

Popson

et al. 2010

Tissue Engineering Part A

183

193

View full text Add to dashboard Cite

show abstract

“…Allogeneic cultured dermal substitutes (CDS) have also been used to treat intractable ulcerated wounds in patients with RDEB (44,47). Apligraf ® (Organogenesis, Canton, MA, USA) is an allogeneic cultured skin substitute consisting of keratinocytes and fibroblasts supported on a scaffold and was initially used in the treatment of venous ulcers.…”

Section: Wound Grafting and Topical Therapiesmentioning

confidence: 99%

Novel and emerging therapies in the treatment of recessive dystrophic epidermolysis bullosa

Rashidghamat

McGrath

2017

IRDR

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“…90 Novel therapeutic approaches include the use of biologic dressings or skin equivalents. 91,[94][95][96] Because heat and excessive sweating are known aggravating factors in EBS, aluminium chloride has been used in an attempt to prevent sweating with mixed results. 97,98 Recently, botulinum toxin has been used to prevent plantar blistering in EBS with some degree of success.…”

Section: Preventionmentioning

confidence: 99%

Epidermolysis Bullosa Simplex

Sprecher

2010

Dermatologic Clinics

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The Use of Tissue-Engineered Skin (Apligraf) to Treat a Newborn With Epidermolysis Bullosa

Cited by 80 publications

References 22 publications

Rapid Anastomosis of Endothelial Progenitor Cell–Derived Vessels with Host Vasculature Is Promoted by a High Density of Cotransplanted Fibroblasts

Rapid Anastomosis of Endothelial Progenitor Cell–Derived Vessels with Host Vasculature Is Promoted by a High Density of Cotransplanted Fibroblasts

Novel and emerging therapies in the treatment of recessive dystrophic epidermolysis bullosa

Epidermolysis Bullosa Simplex

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