2003
DOI: 10.1007/s00299-003-0654-1
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

8
49
1
15

Year Published

2004
2004
2020
2020

Publication Types

Select...
6
3
1

Relationship

0
10

Authors

Journals

citations
Cited by 87 publications
(73 citation statements)
references
References 30 publications
8
49
1
15
Order By: Relevance
“…The effective mannose concentration varied among species, ranging from 0.36 g/l for Arabidopsis (63) to 10-50 g/l for rice (39). Our results confirmed the published data that better transformation efficiency is achieved when lower selection stringency of mannose is combined with a low supplemental carbohydrate concentration (5,72). Therefore, we used a combination of 10 g/l mannose and 5 g/l glucose during the first 6 weeks of the selection.…”
Section: Resultssupporting
confidence: 78%
“…The effective mannose concentration varied among species, ranging from 0.36 g/l for Arabidopsis (63) to 10-50 g/l for rice (39). Our results confirmed the published data that better transformation efficiency is achieved when lower selection stringency of mannose is combined with a low supplemental carbohydrate concentration (5,72). Therefore, we used a combination of 10 g/l mannose and 5 g/l glucose during the first 6 weeks of the selection.…”
Section: Resultssupporting
confidence: 78%
“…Estes resultados de eficiência estão de acordo com os resultados de outros trabalhos, em que a eficiência de transformação utilizando-se epicótilos de laranja "Pêra" foi menor independente da construção gênica utilizada na transformação (AZEVEDO, 2005;BARBOSA-MENDES, 2007;BOSCARIOL, 2004), comprovando que a eficiência de transformação varia com o genótipo e também é influenciada pela construção gênica utilizada, pela habilidade morfogênica e pela afinidade do genótipo com a Agrobacterium tumefaciens GHORBEL et al, 2000;MIYATA et al, 2011 (2005), de 2,5 a 3% para laranja "Hamlin", 0,5% para laranja doce "Pêra" e 3,3 a 4,5% para laranja "Valência". A eficiência de transformação sem gene repórter no trabalho realizado por Barbosa-Mendes (2007), utilizando-se como explantes epicótilos de laranja doce foi de 6,2% para laranja "Hamlin", 2,7% para laranja "Pêra" e 5,6% para laranja "Valência".…”
Section: Discussionunclassified
“…In Citrus sinensis, the best results were obtained when 13 g/L mannose as unique source of carbon was added into the selection media. Mannose combined with other sugars promoted reduction in transformation efficiencies and escapes (Boscariol et al, 2003). Apricot cv.…”
Section: Selection Systems a Critical Stepmentioning
confidence: 99%