The use of the DNA barcode gene 16S mRNA for the clarification of taxonomic problems within the family Sertulariidae (Cnidaria, Hydrozoa)

Abstract

2011). The use of the DNA barcode gene 16S mRNA for the clarification of taxonomic problems within the family Sertulariidae (Cnidaria, Hydrozoa). -Zoologica Scripta, 40, 520-537. The Sertulariidae are the most speciose family of the Hydrozoa, and their systematics are problematic. Here, 77 new 16S mtDNA sequence 'barcodes' of Atlantic and Mediterranean sertulariids were subject to phylogenetic analyses along with another 29 already deposited in Genbank. Amongst the 12 nominal genera and around 40 putative species analysed, several taxonomic inconsistencies and corroborations were highlighted by the genetic data. Patterns of morphological characters between the specimens analysed genetically were extremely congruent with molecular estimates. The genera Sertularia, Dynamena and Symplectoscyphus were found to be polyphyletic, and the genus Sertularella paraphyletic. The validity of Sertularella robusta at a species level was confirmed, along with the exclusion of Sertularelloides cylindritheca from the genus Sertularella. Potential cryptic species were detected within Sertularella fusiformis, Sertularella mediterranea, Sertularella ellisii, Sertularia distans, Dynamena disticha, Thuiaria articulata and Diphasia margareta. Conversely, probable cases of synonomy were revealed for the species pairs: Sertularia cupressina and Sertularia argentea, Amphisbetia minima and Amphisbetia operculata, and Thuiaria thuja and T. articulata. 16S haplotype data also revealed probable phylogeographic structure across localities of the NE Atlantic and Mediterranean. As an alternative 'DNA barcode', the 16S gene has the great advantage of being easily amplified and sequenced across hydrozoan taxa. Furthermore, the marked increase in deposited 16S sequences in public databases facilitates the identification of hydrozoan samples to the level of family, genus, and especially species or even populations, in a manner that is coherent with phenotypic and geographic ⁄ environmental differences.

Similiclava nivea(Cnidaria: Hydrozoa: Similiclavidae): a new family, genus and species of athecate hydroid from the Pacific coast of North America

“…Polymerase chain reaction (PCR) amplification and sequencing followed the protocol outlined in Moura et al (2008). To amplify c. 600 base pairs of the mitochondrial 16S gene, we used the primers SHA (ACGGAATGAACTCAAATCATGT) and SHB (TCGACTGTTTACCAAAA ACATA) (Cunningham and Buss 1993;Moura et al 2011). Forty additional 16S sequences of hydroids, representing nine families, were obtained from GenBank (Appendix 1).…”

DNA barcoding of hydromedusae in northern Beibu Gulf for species identification

“…泛采纳为通用的标准条形码基因片段 (Hebert et al, 2004b;Ward et al, 2005;Hajibabaei et al, 2006;Ratnasingham et al, 2007) Shearer et al, 2002;Hellberg et al, 2006;Huang et al, 2008;McFadden et al, 2011)。然而, Sinniger等 母目和软水母目种类中表现得更为明显 (Shearer et al, 2002;Miglietta et al, 2009;Moura et al, 2011bMoura et al, , 2012 Collins et al, 2008;Moura et al, 2008;Leclère et al, 2009;Bucklin et al, 2011;Moura et al, 2012)。同 时, 它还常作为一种有效的分子标记, 用以区分形 态差异细微、 不可辨识或尚未描述的种类 Miglietta et al, 2009;Miranda et al, 2010;Moura et al, 2011a, b)。Schuchert等(2006, 2007, 2008a, b, 2009 …”

First record of Aequorea macrodactyla (Cnidaria, Hydrozoa) from the Israeli coast of the eastern Mediterranean Sea, an alien species indicating invasive pathways

“…It is considered to be a gene that is much easier to amplify and is more available in the GenBank database (Cantero et al 2010;Moura et al 2011;Laakmann and Holst 2014;Zheng et al 2014).…”