The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps

Martinelli, Arturo B.; Waichert, Cecília; Barbosa, Diego N.; Fagundes, Valéria; Azevedo, Celso O.

doi:10.1590/0001-3765201720160825

Cited by 8 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taxonomic characters related to the male genitalia and hypopygium were examined after dissection. Genitalia and hypopygium were diaphanized according to Martinelli et al (2017). After this step, the genitalia were transferred into a microvial filled with glycerin for preservation.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Integrative taxonomy solves taxonomic impasses: a case study from Epyrinae (Hymenoptera, Bethylidae)

Colombo

Tribull

Waichert

et al. 2022

Systematic Entomology

Self Cite

View full text Add to dashboard Cite

We apply integrative taxonomy to solve a historical taxonomic impasse in a flat wasp group (Hymenoptera, Bethylidae). The phylogeny of Epyrinae is inferred with parsimony analyses under equal and implied weights, maximum likelihood and Bayesian inference using the nuclear markers 18S and 28S, the mitochondrial genes 16S, cytochrome oxidase subunit I and cytochrome B and 232 morphological characters. This is the first phylogenetic study to examine all 42 epyrine genera, including junior synonyms and extinct taxa, applying a combined molecular and morphological approach. This study considered 186 terminal taxa with 166 representing the ingroup taxa and 20 outgroup taxa. All resulting trees of the combined analyses recovered Anisepyris Kieffer, Aspidepyris Evans, Bakeriella Kieffer, Disepyris Kieffer, Laelius Ashmead and Trachepyris Kieffer as monophyletic; Chlorepyris Kieffer, Epyris Westwood, Formosiepyris Terayama and Holepyris Kieffer are nonmonophyletic. Calyozina Enderlein is recovered as an independent lineage in implied weights, Bayesian and maximum likelihood topologies. Here, we describe three new genera and revalidate six genera, five of them junior synonyms of Epyris and one of Holepyris. In addition, we analysed the presence/absence of a sulcus between the mesoscuto-scutellar foveae and the shape of mesoscuto-scutellar foveae as the most important taxonomic characters for Epyrinae using ancestral state reconstruction. Finally, we provide a checklist of all species of Epyrinae, compiled via direct observation of the type, illustrations, or literature and an updated and illustrated taxonomic key for all epyrine genera.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Taxonomic characters related to the male genitalia and hypopygium were examined after dissection. Genitalia and hypopygium were diaphanized according to Martinelli et al (2017).…”

Section: Terminology and Treatmentmentioning

confidence: 99%

Integrative taxonomy solves taxonomic impasses: a case study from Epyrinae (Hymenoptera, Bethylidae)

Colombo

Tribull

Waichert

et al. 2022

Systematic Entomology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Studies about biological aspects and parasitoid specificity of P. obliqua (Murgas 2005(Murgas , 2008, show that most of the adults that emerge from the larvae are females, that could be due to a infection of the symbiotic bacteria, Wolbachia pipientis (Hertig), that is lethal to male parasitoids (stoutahaMer 1997). The presence of Wolbachia in DNA of Bethylidae was reported by Martinelli et al (2017). This, combined with the fact that Prosierola rarely is captured, could be the reason why so few males were found.…”

Section: Discussionmentioning

confidence: 92%

Taxonomic additions of the Brazilian fauna of Prosierola (Hymenoptera, Bethylidae)

Pereira,

Brito,

Azevedo

2023

EntomoBrasilis

View full text Add to dashboard Cite

Prosierola is characterized by having a pair of conspicuous anteromedial metapostnotal foveae on the metapectal-propodeal disc. Little is known about this genus because it is rarely sampled in collections. Therefore, the main goal of this contribution is to expand the knowledge of intraspecific morphological variations and geographical distribution of its species that occur in Brazil. We sorted material from the main collections and obtained 69 specimens of Prosierola nasalis (Westwood) recorded for the first time from Maranhão, Minas Gerais, Pará, Rio Grande do Norte and Rio Grande do Sul; one of Prosierola obliqua Evans recorded for the first time from Distrito Federal; five of Prosierola rotunda Schiffler & Azevedo recorded for the first time from Pará; and 13 of Prosierola rufescens Evans recorded for the first time from Brazil (Amazonas, Bahia, Espírito Santo, Minas Gerais). The mesoscuto-scutellar foveae placed on the dorsal surface of the mesoscutellum shown to be highly variable in size, distance, and shape at both the intra- and interspecific levels.

show abstract

“…The hypopygium and male genitalia were diaphanised according to Martinelli et al (2017). Drawings were made using a camera lucida attached to a Leica DM2500 light microscope and digitalised afterwards with an image editor.…”

Section: Methodsmentioning

confidence: 99%

Revision of Nothepyris Evans, 1973 (Hymenoptera: Bethylidae), with description of four new species

Colombo

Azevedo

2023

Austral Entomology

Self Cite

View full text Add to dashboard Cite

Nothepyris Evans, 1973 is revised. Six species are recognised, two being previously described species, Nothepyris brasiliensis Evans, 1973 and Nothepyris pretiosus Colombo & Azevedo, 2019, and four species described and illustrated as new, Nothepyris beedrill sp. nov., Nothepyris combee sp. nov., Nothepyris durant sp. nov. and Nothepyris vespiquen sp. nov. The species Nothepyris sulcatus (Azevedo, 1999) syn. nov. is proposed as a junior synonym of Nothepyris brasiliensis. For the first time, males of this genus are described, and the morphology of the male genitalia is compared and discussed with other Scleroderminae. An updated taxonomic key to all species for both sexes is presented.

show abstract

The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps

Cited by 8 publications

References 7 publications

Integrative taxonomy solves taxonomic impasses: a case study from Epyrinae (Hymenoptera, Bethylidae)

Integrative taxonomy solves taxonomic impasses: a case study from Epyrinae (Hymenoptera, Bethylidae)

Taxonomic additions of the Brazilian fauna of Prosierola (Hymenoptera, Bethylidae)

Revision of Nothepyris Evans, 1973 (Hymenoptera: Bethylidae), with description of four new species

Contact Info

Product

Resources

About