The use of molecular techniques to characterize the microbial communities in contaminated soil and water

Malik, Shaukat Iqbal; Beer, Michael; Megharaj, Mallavarapu; Naidu, Ravi

doi:10.1016/j.envint.2007.09.001

Cited by 174 publications

(107 citation statements)

References 165 publications

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“…As an alternative to cultivation-based techniques, phospholipids fatty acid (PLFA) analysis is increasingly common in studies of microbial communities (Malik et al, 2008). Phospholipids are essential membrane components of all living cells.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of microbial community structure in different filter media of constructed wetland

Zhou

Tao

et al. 2010

Journal of Environmental Sciences

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Section: Introductionmentioning

confidence: 99%

Comparative study of microbial community structure in different filter media of constructed wetland

Zhou

Tao

et al. 2010

Journal of Environmental Sciences

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“…Aunque se ha reportado que sólo 0.1 a 1 % de la población microbiana puede detectarse en muestras ambientales mediante técnicas clásicas de cultivo in vitro (Malik et al 2008), actualmente se efectúan intentos para mejorar estos porcentajes de detección (Tanaka 2014). No obstante, se ha sugerido que la carga bacteriana podría superar el billón de organismos por gramo de suelo pertenecientes a más de mil diferentes especies (Torsvik y Ǿvreas 2002).…”

Section: Cuadro I Variación De Los Parámetros Bioquímicos En Muestraunclassified

“…Como es sabido, en la actualidad existen técnicas moleculares (mediante la identificación del gen ARNr16S) y bioquímicas (perfil de ácidos grasos metil esterificados, entre otras) que permiten evaluar la diversidad de microorganismos cultivables y no cultivables de una manera más exhaustiva (Malik et al 2008). Sin embargo, este trabajo sólo pretende mostrar los cambios en la diversidad de las bacterias cultivables, asociados con la presencia de metales en concentraciones que se estiman permisibles según la legislación venezolana.…”

Section: Cuadro I Variación De Los Parámetros Bioquímicos En Muestraunclassified

Efectos Sobre Parámetros Bioquímicos Y Biológicos Bacterianos en Suelos Que Contienen Concentraciones Permisibles De Metales

Matute

Guerrero

Durán

et al. 2018

Rev. Int. Contam. Ambie.

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Palabras clave: diversidad bacteriana, carga bacteriana, contaminación con metales, normativas ambientales RESUMEN La contaminación de ambientes naturales por metales es básicamente producto de actividades antrópicas, tales como siderurgia, minería, galvanoplastia y Curtiembre es el lugar donde ocurre el proceso de curtido de pieles, no el proceso en sí de pieles y cuero. Como consecuencia, los microorganismos en los ambientes impactados se someten a condiciones estresantes, provocando una disminución de la biomasa y diversidad biológica, particularmente la bacteriana. En este estudio se reportan disminuciones estadísticamente significativas en el contenido de carbono orgánico total (70 %), actividad ureásica (54 %), biomasa bacteriana (47 %) y carga bacteriana (30-80 %) en suelos que contienen metales en niveles considerados permisibles por la legislación venezolana. El cociente respiración basal/biomasa microbiana se incrementó en 58 % respecto al suelo testigo. La diversidad bacteriana se redujo y se lograron aislar sólo 12 morfotipos en placas de agar LB suplementadas con sales ) fueron parámetros determinantes en la reducción de la diversidad y carga bacterianas. Los datos reportados deberían constituir un llamado de alerta a la autoridad ambiental venezolana para revisar y establecer los niveles permisibles de metales en ambientes naturales, de tal manera de que los parámetros bioquímicos y fisiológicos del suelo no se afecten negativamente; adicionalmente, la carga y diversidad microbianas deberían ser incluidas como bioindicadores de salud para suelos.Key words: bacterial diversity, bacterial load, contamination by metals, environmental normative ABSTRACT Contamination of natural environments by metals is basically due to anthropogenic activities such as iron and steel industries, mining, electroplating and tannery. As a A.J. Armado-Matute et al. 442consequence, proliferating microbes in such environments are subjected to stress conditions which induce decreases of the microbial diversity and biomass, particularly the bacterial one. This study reports statistical significant reductions of the total organic carbon content (70 %), urease activity (54 %), microbial biomass (47 %), and bacterial load (3080 %) in soil samples containing permissible levels of metals according to the Venezuelan legislation. Besides, the basal respiration/microbial biomass ratio increased 58 % respect to the witness soil. The bacterial diversity was severally reduced and only 12 bacterial morphotypes were isolated from agar LB plates supplemented with Cr ) were the most important parameters involved in reducing the bacterial diversity and load. The reported data should constitute an alarm call to the Venezuelan environmental authority to revise and set the metal permissible levels in natural environments, in such a way the soil biochemical and physiological parameters are not negatively affected; besides, microbial load and diversity should be included as health bioindicators to soils. INTRODUCCIÓNEl suelo es uno de ambientes na...

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“…Nucleic acid extraction from target niches and amplifi cation of the 16S rRNA gene by polymerase chain reaction (PCR) has proved extremely useful in assessing the microbial community [21,22]. Changes in the population dynamics can also be assessed by analyzing the microbial diversity at different time points or after treatment/change in carbon source of the niche [23].…”

Section: Role Of Microbes/microbial Community In Bioremediationmentioning

confidence: 99%

Genomic tools in bioremediation

Kapley

Purohit

2009

Indian J Microbiol

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Bioremediation is a process that uses microorganisms or their enzymes to remove pollutants from the environment. Generally, bioremediation technologies can be classifi ed as in situ or ex situ. In situ bioremediation involves treating the contaminated material at the site while ex situ involves the removal of the contaminated material to be treated elsewhere. Like so much else in biology, the ease and availability of genomic data has created a new level of understanding this system. Bioremediation capabilities of the microbial population can be analyzed; not only by physiological parameters, but also by the use of genomic tools, and effi cient remediation strategies can be planned. PCR and DNA-or oligonucleotide-based microarray technology is a powerful functional genomics tool that allows researchers to view the physiology of a living cell from a comprehensive and dynamic molecular perspective. This paper explores the use of such tools in bioremediation process.

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The use of molecular techniques to characterize the microbial communities in contaminated soil and water

Cited by 174 publications

References 165 publications

Comparative study of microbial community structure in different filter media of constructed wetland

Comparative study of microbial community structure in different filter media of constructed wetland

Efectos Sobre Parámetros Bioquímicos Y Biológicos Bacterianos en Suelos Que Contienen Concentraciones Permisibles De Metales

Genomic tools in bioremediation

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