The use of micro-CT to evaluate cortical bone geometry and strength in nude rats: Correlation with mechanical testing, pQCT and DXA

Bagi, Cedo M.; Hanson, Nels; Andresen, Catharine J.; Pero, Richard W.; Lariviere, Roland; Turner, Charles H.; Laib, Andres

doi:10.1016/j.bone.2005.07.028

Cited by 123 publications

(88 citation statements)

References 46 publications

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“…Quantitative computed tomography (qCT), peripheral quantitative CT (pQCT) and micro CT (μCT) are additional techniques that are superior to Xray in assessing bone geometry, mass and structure. X-ray with/without pQCT is the method of choice for in vivo follow-up due to its good correlation with the mechanical testing of bone strength [104]. The strength of bone and callus assessed via the 3-and/or 4-point bending method (or torsional testing performed ex vivo) supplements the assessment of bone regeneration quality and provides opportunities to correlate and accurately interpret radiologic and other data (serum biomarkers, histology).…”

Section: Methods and Technologymentioning

confidence: 99%

The rational use of animal models in the evaluation of novel bone regenerative therapies

Perić¹,

Dumić-Čule²,

Grčević³

et al. 2015

Bone

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126

116

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Section: Methods and Technologymentioning

confidence: 99%

The rational use of animal models in the evaluation of novel bone regenerative therapies

Perić¹,

Dumić-Čule²,

Grčević³

et al. 2015

Bone

Self Cite

126

116

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“…The outer and inner perimeter of the cortical midshaft was determined by a three-dimensional triangulation of the bone surface (BS) of the 20 slices, and cortical thickness was calculated as described. 24 …”

Section: Cortical Bone Assessment By Microctmentioning

confidence: 99%

Platelet Dysfunction and a High Bone Mass Phenotype in a Murine Model of Platelet-Type von Willebrand Disease

Suva

Hartman

Dilley

et al. 2008

The American Journal of Pathology

110

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The platelet glycoprotein Ib-IX receptor binds surfacebound von Willebrand factor and supports platelet adhesion to damaged vascular surfaces. A limited number of mutations within the glycoprotein Ib-IX complex have been described that permit a structurally altered receptor to interact with soluble von Willebrand factor, and this is the molecular basis of platelet-type von Willebrand disease. We have developed and characterized a mouse model of platelet-type von Willebrand disease (G233V) and have confirmed a platelet phenotype mimicking the human disorder. The mice have a dramatic increase in splenic megakaryocytes and splenomegaly. Recent studies have demonstrated that hematopoetic cells can influence the differentiation of osteogenic cells. Thus, we examined the skeletal phenotype of mice expressing the G233V variant complex. At 6 months of age, G233V mice exhibit a high bone mass phenotype with an approximate doubling of trabecular bone volume in both the tibia and femur. Serum measures of bone resorption were significantly decreased in G233V animals. With decreased bone resorption, cortical thickness was increased, medullary area decreased, and consequently, the mechanical strength of the femur was significantly increased. Using ex vivo bone marrow cultures, osteoclast-specific staining in the G233V mutant marrow was diminished, whereas osteoblastogenesis was unaffected. These studies provide new insights into the relationship between the regulation of megakaryocytopoiesis and bone mass. (Am J

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“…Source energy was 70 kV, current was 160 mA, scan time was 20.6 min, and voxel resolution was 8 mm. 28,29 A fixed global threshold method was used in this study based on manufacturer recommendations and preliminary studies, which showed that mineral variation between groups was not high enough to warrant adaptive thresholds. Porosity was calculated as (1-BV/TV), where BV/ TV is the relative bone volume as measured by voxel counting.…”

Section: Microct Analysismentioning

confidence: 99%

Effects of ovariectomy on bone turnover, porosity, and biomechanical properties in ovine compact bone 12 months postsurgery

Kennedy

Brennan

Rackard

et al. 2009

Journal Orthopaedic Research

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Compact bone makes up approximately 80% of the human skeleton by mass; but there are little data available on the effects of increased bone turnover on compact bone mechanical and material properties. This study addresses this question by measuring intracortical remodeling, resorption cavity number, and porosity in an ovariectomized (OVX) sheep model, and measures changes in biomechanical properties. Thirty-eight sheep were divided into two groups. Group 1 were controls (n ¼ 19), and Group 2 were ovariectomized (OVX; n ¼ 19). Fluorochrome dyes were administered intravenously to both groups at five time points over 12 months post-OVX to label sites of bone turnover. At 12 months post-OVX all animals were euthanized. Samples were harvested from the left metatarsal and were analyzed for intracortical bone turnover at five time points, the number of resorption cavities, and the level of intracortical porosity. The effects of these parameters on bone biomechanical properties were then measured. Bone turnover was increased in the OVX group at 6, 9, and 12 months (p < 0.05). Resorption was also higher in the OVX group at 12 months (p < 0.05). Furthermore, porosity was significantly increased in the OVX group at 12 months (p < 0.05). Stiffness and yield strength were reduced in the OVX group compared to controls (p ¼ 0.05). Ultimate compressive strength and work to fracture did not differ between groups. These findings provide new insights into the mechanisms and effects of increased bone turnover on bone material and microstructural properties. Keywords: bone turnover; porosity; ovariectomy; ovine animal model Bone turnover is an important determinant of the structural integrity of bone. It is a crucial process of tissue renewal that normally occurs at an optimal physiological rate for maximum bone strength in a healthy individual. 1 Any change to the optimal bone turnover rate, such as the increase that occurs following ovariectomy (OVX) or menopause, has the potential to dramatically change the bone at microstructural and material levels. High bone turnover can have the effect of increasing remodeling spaces, accelerating bone loss, increasing stress concentrations, and increasing cortical porosity, each of which can undermine bone mechanical properties. A precise definition of bone quality remains elusive, however; bone turnover and microarchitecture are considered to be two important contributors to this phenomenon along with mineralization, microdamage, and collagen content. Thus, these parameters are important considerations in the pathophysiology of skeletal diseases such as osteoporosis and the mechanisms that underlie fracture. 2 Previous studies have shown that bone turnover has a direct influence on fracture risk. 3 It is thought that compact bone is affected by accelerated turnover in two ways. First, more mineralized bone is removed and replaced with younger, less mineralized bone, which reduces the material's local stiffness. Second, the presence of more resorption cavities creates stress concen...

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The use of micro-CT to evaluate cortical bone geometry and strength in nude rats: Correlation with mechanical testing, pQCT and DXA

Cited by 123 publications

References 46 publications

The rational use of animal models in the evaluation of novel bone regenerative therapies

The rational use of animal models in the evaluation of novel bone regenerative therapies

Platelet Dysfunction and a High Bone Mass Phenotype in a Murine Model of Platelet-Type von Willebrand Disease

Effects of ovariectomy on bone turnover, porosity, and biomechanical properties in ovine compact bone 12 months postsurgery

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