The Use of Lipid Peroxidation Expression as a Biomarker for the Molecular Damage in the Critically Ill Polytrauma Patient

Horhat, Florin George; Rogobete, Alexandru Florin; Păpurică, Marius; Săndesc, Dorel; Tănăsescu, Sonia; Dumitrașcu, Victor; Licker, Monica; Niţu, Răzvan; Cradigati, Carmen Alina; Sărăndan, Mirela; Borugă, Ovidiu; Crisan, Ciprian D; Ungureanu, Emil; Popa, Daniela M; Popovici, Sonia Elena; Bedreag, Ovidiu Horea

doi:10.7754/clin.lab.2016.160306

Cited by 14 publications

(15 citation statements)

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“…We observed no significant differences in the number of patients requiring mechanical ventilation for more than 96 hours between the two groups. One explanation for this could be the high number of patients with severe thoracic trauma, as well as an increased percentage of pulmonary infections [21][22][23][24][25][26][27].…”

Section: Discussionmentioning

confidence: 99%

Analysis of oxidative stress-related markers in critically ill polytrauma patients: An observational prospective single-center study

Sandesc

Rogobete

Bedreag

et al. 2018

Bosn J of Basic Med Sci

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Critically ill polytrauma patients have increased production of free radicals (FRs) and consequent alterations in biochemical pathways, as well as disruption of cellular integrity, due to increased lipid peroxidation. The aim of this study was to investigate several biomarkers associated with increased oxidative stress in critically ill polytrauma patients, and to evaluate the effect of antioxidant treatment on the clinical outcome in these patients. A total of 67 polytrauma patients from an intensive care unit met the selection criteria. Antiox group included 35/67 patients who received antioxidant therapy, while 32/67 patients without antioxidant treatment were considered as control group. Antioxidant therapy consisted of simultaneous administration of Vitamin C (sodium ascorbate) and N-acetylcysteine, through continuous intravenous infusion. Clinical and paraclinical evaluation of the patients was performed daily until discharge or death. At admission, laboratory parameters did not differ significantly between two groups. At discharge/upon death, statistically significant differences in favor of Antiox group were observed in the following parameters: thrombocytes, activated partial thromboplastin time, prothrombin time, total bilirubin, total cholesterol, high-density lipoproteins, low-density lipoproteins, erythrocyte sedimentation rate, interleukin 6 (all p = 0.0001), total protein (p = 0.0005), serum albumin (p = 0.0004), lactate dehydrogenase (p = 0.0006), and C-reactive protein (p = 0.0014). Starting from day 5, the APACHE II score was significantly decreased in Antiox versus control group (p < 0.05). Finally, the sepsis incidence and mortality rate were significantly lower in Antiox group (p < 0.05). Decreasing the level of oxidative stress by antioxidant substances significantly correlated with a better prognosis and outcome in our patients. Further studies should elucidate more clearly the mechanism of action of antioxidants in critically ill polytrauma patients.

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Section: Discussionmentioning

confidence: 99%

Analysis of oxidative stress-related markers in critically ill polytrauma patients: An observational prospective single-center study

Sandesc

Rogobete

Bedreag

et al. 2018

Bosn J of Basic Med Sci

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“…In hypermetabolic states like inflammation and sepsis, the oxidant/antioxidant balance is significantly disrupted, with an exaggerated increase in FR expressions. This major imbalance that becomes systemic in a very short period of time is called oxidative stress (OS), and is involved in the majority of biochemical pathways [90][91][92][93][94][95].…”

Section: Cannabinoid Signaling System and Redox Activity-linked With mentioning

confidence: 99%

Cannabis Sativa Revisited—Crosstalk between microRNA Expression, Inflammation, Oxidative Stress, and Endocannabinoid Response System in Critically Ill Patients with Sepsis

Dinu

Rogobete

Bratu

et al. 2020

Cells

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Critically ill patients with sepsis require a multidisciplinary approach, as this situation implies multiorgan distress, with most of the bodily biochemical and cellular systems being affected by the condition. Moreover, sepsis is characterized by a multitude of biochemical interactions and by dynamic changes of the immune system. At the moment, there is a gap in our understanding of the cellular, genetic, and molecular mechanisms involved in sepsis. One of the systems intensely studied in recent years is the endocannabinoid signaling pathway, as light was shed over a series of important interactions of cannabinoid receptors with biochemical pathways, specifically for sepsis. Furthermore, a series of important implications on inflammation and the immune system that are induced by the activity of cannabinoid receptors stimulated by the delta-9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) have been noticed. One of the most important is their ability to reduce the biosynthesis of pro-inflammatory mediators and the modulation of immune mechanisms. Different studies have reported that cannabinoids can reduce oxidative stress at mitochondrial and cellular levels. The aim of this review paper was to present, in detail, the important mechanisms modulated by the endocannabinoid signaling pathway, as well as of the molecular and cellular links it has with sepsis. At the same time, we wish to present the possible implications of cannabinoids in the most important biological pathways involved in sepsis, such as inflammation, redox activity, immune system, and epigenetic expression.

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“…Redox balance is significantly affected, with significant quantities of free radicals being released. In this case, the metabolic imbalance is strongly affected at a molecular level, with the free radicals being involved in a series of protein and lipid denaturation reactions [12,[44][45][46]. Moreover, the redox protein and lipid denaturation reactions lead to the release of other reactive species, leading to augmentation of the pro-oxidative cascade [16,47].…”

Section: Molecular and Pathophysiological Aspects Of Metabolismmentioning

confidence: 99%

Assessment of Metabolic and Nutritional Imbalance in Mechanically Ventilated Multiple Trauma Patients: From Molecular to Clinical Outcomes

et al. 2019

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The critically ill polytrauma patient is characterized by a series of metabolic changes induced by inflammation, oxidative stress, sepsis, and primary trauma, as well as associated secondary injuries associated. Metabolic and nutritional dysfunction in the critically ill patient is a complex series of imbalances of biochemical and genetic pathways, as well as the interconnection between them. Therefore, the equation changes in comparison to other critical patients or to healthy individuals, in which cases, mathematical equations can be successfully used to predict the energy requirements. Recent studies have shown that indirect calorimetry is one of the most accurate methods for determining the energy requirements in intubated and mechanically ventilated patients. Current research is oriented towards an individualized therapy depending on the energy consumption (kcal/day) of each patient that also takes into account the clinical dynamics. By using indirect calorimetry, one can measure, in real time, both oxygen consumption and carbon dioxide production. Energy requirements (kcal/day) and the respiratory quotient (RQ) can be determined in real time by integrating these dynamic parameters into electronic algorithms. In this manner, nutritional therapy becomes personalized and caters to the patients’ individual needs, helping patients receive the energy substrates they need at each clinically specific time of treatment.

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The Use of Lipid Peroxidation Expression as a Biomarker for the Molecular Damage in the Critically Ill Polytrauma Patient

Cited by 14 publications

References 0 publications

Analysis of oxidative stress-related markers in critically ill polytrauma patients: An observational prospective single-center study

Analysis of oxidative stress-related markers in critically ill polytrauma patients: An observational prospective single-center study

Cannabis Sativa Revisited—Crosstalk between microRNA Expression, Inflammation, Oxidative Stress, and Endocannabinoid Response System in Critically Ill Patients with Sepsis

Assessment of Metabolic and Nutritional Imbalance in Mechanically Ventilated Multiple Trauma Patients: From Molecular to Clinical Outcomes

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