The use of intestinal brush border membrane vesicles for comparative studies of glucose and 2-amino isobutyric acid transport by four species of marine teleost

Dietary lipid level affects fatty acid composition and hydrolase activities of intestinal brush border membrane in seabass

“…Moreover, the contamination of this BBM fraction by basolateral membranes was reduced to traces, based on assays of Na + -K + -ATPase which could be considered as a marker of the basolateral membranes (Mircheff et al, 1985). The degree of purification of the BBM fraction extracted from the intestinal scraping corresponded to that obtained by intestinal scraping in mammals (Malathi et al, 1979) and adult fish (Crane et al, 1979;Bogé et al, 1982). The recovery of brush border membranes, appraised by the relative activity of alkaline phosphatase -brush border membrane/homogenate -was similar in all dietary groups.…”

Dietary lipid level affects fatty acid composition and hydrolase activities of intestinal brush border membrane in seabass

“…Moreover, the contamination of this BBM fraction by basolateral membranes was reduced to traces, based on assays of Na + -K + -ATPase which could be considered as a marker of the basolateral membranes (Mircheff et al, 1985). The degree of purification of the BBM fraction extracted from the intestinal scraping corresponded to that obtained by intestinal scraping in mammals (Malathi et al, 1979) and adult fish (Crane et al, 1979;Bogé et al, 1982). The recovery of brush border membranes, appraised by the relative activity of alkaline phosphatase -brush border membrane/homogenate -was similar in all dietary groups.…”

Comparative enzyme activities of the intestinal brush border membranes of the herbivorous mudskipperBoleophthalmus pectinirostrisand the carnivorous Chinese black sleeperBostrichthys sinensis

“…BBM purity in each intestinal section was evaluated by the degree of enrichment of ALP specific activity, which is regarded as the most classical marker of the BBM (Boge et al., 1982). The enrichment factor was calculated as the ratio of the specific activity in the BBM to the specific activity in the homogenate.…”

Development and response to a diet change of some digestive enzymes in sea bass (Dicentrarchus labrax) larvae

“…The importance of this phase of digestion (hydrolytic and absorptive mechanisms) has been clearly proved, particularly in mammals (Matthews and Adibi 1975;Kim et al 1979;Koretz and Meyer 1980) but also in juvenile fish (Bog6 et al 1982;Ferraris and Ahearn 1984;Avella et al 1992).…”

Dietary lipid level affects fatty acid composition and hydrolase activities of intestinal brush border membrane in seabass

“…Moreover, the contamination of this BBM fraction by basolateral membranes was reduced to traces, based on assays of Na + -K + -ATPase which could be considered as a marker of the basolateral membranes (Mircheff et al, 1985). The degree of purification of the BBM fraction extracted from the intestinal scraping corresponded to that obtained by intestinal scraping in mammals (Malathi et al, 1979) and adult fish (Crane et al, 1979;Bogé et al, 1982). The recovery of brush border membranes, appraised by the relative activity of alkaline phosphatase -brush border membrane/homogenate -was similar in all dietary groups.…”

Comparative enzyme activities of the intestinal brush border membranes of the herbivorous mudskipperBoleophthalmus pectinirostrisand the carnivorous Chinese black sleeperBostrichthys sinensis

“…BBM purity in each intestinal section was evaluated by the degree of enrichment of ALP specific activity, which is regarded as the most classical marker of the BBM (Boge et al., 1982). The enrichment factor was calculated as the ratio of the specific activity in the BBM to the specific activity in the homogenate.…”

Development and response to a diet change of some digestive enzymes in sea bass (Dicentrarchus labrax) larvae

“…The importance of this phase of digestion (hydrolytic and absorptive mechanisms) has been clearly proved, particularly in mammals (Matthews and Adibi 1975;Kim et al 1979;Koretz and Meyer 1980) but also in juvenile fish (Bog6 et al 1982;Ferraris and Ahearn 1984;Avella et al 1992).…”

Dietary lipid level affects fatty acid composition and hydrolase activities of intestinal brush border membrane in seabass

“…Moreover, the contamination of this BBM fraction by basolateral membranes was reduced to traces, based on assays of Na + -K + -ATPase which could be considered as a marker of the basolateral membranes (Mircheff et al, 1985). The degree of purification of the BBM fraction extracted from the intestinal scraping corresponded to that obtained by intestinal scraping in mammals (Malathi et al, 1979) and adult fish (Crane et al, 1979;Bogé et al, 1982). The recovery of brush border membranes, appraised by the relative activity of alkaline phosphatase -brush border membrane/homogenate -was similar in all dietary groups.…”

Comparative enzyme activities of the intestinal brush border membranes of the herbivorous mudskipperBoleophthalmus pectinirostrisand the carnivorous Chinese black sleeperBostrichthys sinensis

“…BBM purity in each intestinal section was evaluated by the degree of enrichment of ALP specific activity, which is regarded as the most classical marker of the BBM (Boge et al., 1982). The enrichment factor was calculated as the ratio of the specific activity in the BBM to the specific activity in the homogenate.…”

Development and response to a diet change of some digestive enzymes in sea bass (Dicentrarchus labrax) larvae

“…The importance of this phase of digestion (hydrolytic and absorptive mechanisms) has been clearly proved, particularly in mammals (Matthews and Adibi 1975;Kim et al 1979;Koretz and Meyer 1980) but also in juvenile fish (Bog6 et al 1982;Ferraris and Ahearn 1984;Avella et al 1992).…”