The Use of Impression Cytology in Ocular Surface Diseases

Turan, Gülay; Oltulu, Pembe; Turan, Meydan; Oltulu, Refik

doi:10.30733/std.2019.01241

Cited by 14 publications

(13 citation statements)

References 5 publications

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“…CIC is a useful technique which enables clinicians to evaluate the epithelial cell morphology, assess nuclear and cytoplasmic characteristics, and quantify the goblet cell density in the conjunctiva. [ 9 10 ] We found that there was more squamous metaplasia and goblet cell loss in the bulbar conjunctiva in Group 1 and Group 2 when compared with control subjects. Indeed, the CIC score was higher in Group 1 (severe KC) than in Group 2 (mild KC) but the difference was statistically insignificant.…”

Section: Discussionmentioning

confidence: 78%

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Dry eye disease and ocular surface characteristics in patients with keratoconus

Mirza

Oltulu

et al. 2022

Saudi J Ophthalmol

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Section: Discussionmentioning

confidence: 78%

“…Conjunctival impression cytology (CIC) is a diagnostic test for DED and squamous metaplasia in ocular surface disease. [ 9 10 ] CIC is a minimally invasive biopsy method of obtaining specimens from the conjunctiva and assessing the density of conjunctival goblet cells.…”

Section: Introductionmentioning

confidence: 99%

Dry eye disease and ocular surface characteristics in patients with keratoconus

Mirza

Oltulu

et al. 2022

Saudi J Ophthalmol

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“…Impression cytology samples were collected using nitrocellulose acetate filter papers (Filter‐Lab, Filtros Anoia S.A., Barcelona, Spain) by applying light pressure for 10 s on the inferior temporal bulbar conjunctiva at a distance of 3 mm from the limbus. Specimens were fixed in a solution containing glacial acetic acid, 37% formaldehyde, and 70% ethanol with a 1:1:20‐volume ratio and stained with hematoxylin‐eosin and periodic acid‐Schiff stains 22 . The CIC specimens were evaluated and graded (Grade 0, 1, 2, or 3) with the Nelson grading system by a specialized pathologist (P.O.)…”

Section: Methodsmentioning

confidence: 99%

“…Specimens were fixed in a solution containing glacial acetic acid, 37% formaldehyde, and 70% ethanol with a 1:1:20-volume ratio and stained with hematoxylin-eosin and periodic acid-Schiff stains. 22 The CIC specimens were evaluated and graded (Grade 0, 1, 2, or 3) with the Nelson grading system by a specialized pathologist (P.O.) who was blinded to the groupings and clinical information.…”

Section: Cic Samples and Histopathological Evaluationmentioning

confidence: 99%

Impact of unilateral trigeminal neuralgia on bilateral ocular surface alterations

et al. 2022

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Objective: To evaluate ocular surface alterations in both eyes of patients with unilateral trigeminal neuralgia (TN) compared with controls.Background: Corneal nerves mainly originate from the trigeminal nerve, and neurosensory abnormalities are important factors in ocular surface alterations and dry eye etiopathogenesis.Methods: Twenty-four patients with idiopathic unilateral TN and 24 healthy controls with similar sex and age distributions were included in this cross-sectional study conducted from February 15 to September 15, 2021. The eyes on the affected sides of the patients with TN were treated as Group 1, their contralateral eyes as Group 2, and the right eyes of the controls as Group 3. All participants were evaluated for tear film and ocular surface using the Schirmer 1 test, tear breakup time (TBUT), Ocular Surface Disease Index (OSDI) score, and conjunctival impression cytology grading. Results:The mean (SD) ages of the patients with TN (17 of 24 females, 70.8%) and controls (15 of 24 females, 62.5%) were 49.7 (11.7) and 48.5 (9. 6) years, respectively.The median [25th, 75th percentile] Schirmer 1 test results in Groups 1, 2, and 3 were 5.

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“…These mucins, along with the water and electrolytes secreted by the lacrimal gland and the lipids produced by the Meibomian glands, form the tear lm. Alterations in the function of goblet cells lead to changes in tear composition that can result in different pathologies [7]. A histological classi cation of squamous metaplasia on the ocular surface is signi cant because doctors observe patients, classify and treat them based on their clinical manifestations.…”

Section: Introductionmentioning

confidence: 99%

A Study On Conjunctival Impression Cytology in Patients with Early Multiple Sclerosis

El-Metwaly

Aly

Mohamed

et al. 2022

Preprint

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Objective The current study aims to evaluate the utility of impression cytology in the diagnosis of ocular surface alterations in multiple sclerosis patients as well as to connect impression cytology results with symptoms and clinical evaluation data. Patients and Methods: The study included 60 patients [120 eyes] who were divided into 3 groups: Group 1: included 20 participants (40 eyes) with early-diagnosed multiple sclerosis; group 2: included 20 participants (40 eyes) with multiple sclerosis who are already receiving treatment; and group 3: included 20 participants (40 eyes) who were randomly assigned to an age-matched control group who had no ocular or systemic pathologies. In people aged 20 to 40, an ocular tear film was evaluated. All patients finished the routine ophthalmological assessment, fluorescein tear breakup time [TBUT], Schirmer test, and conjunctival impression cytology [CIC]. Results Most of the early group patients showed grades 1, 2 and 3 (20%, 35% and 30% respectively) in CIC results in 85% of the eyes, whilst in the chronic group patients showed grades 1, 2 and 3 (35%, 25%and 15% respectively) in CIC results in 75% of the eyes, while in the control subjects, impression cytology results showed that all cases were grade 0 (100%) with a highly statistically significant difference with P-value < 0.001. As regarding the mean scores for the TBUT and Schirmer scores for the control group patients, they were (13.7 ± 1.488s) and (13.325 ± 1.5424mm), respectively, whilst the mean scores for the TBUT and Schirmer scores for the early group patients were (7.85 ± 2.1068s) and (7.525 ± 1.9347mm), respectively, while the mean scores for the TBUT and Schirmer scores for the chronic group patients were (9.625 ± 3.3258s) and (9.8 ± 3.4877mm) with a highly statistically significant difference with a P-value < 0.001. Conclusion Impression cytology is a non-invasive procedure for examining ocular surface cell morphology that can be used to identify and assess the severity of conjunctival alterations in people with multiple sclerosis.

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The Use of Impression Cytology in Ocular Surface Diseases

Cited by 14 publications

References 5 publications

Dry eye disease and ocular surface characteristics in patients with keratoconus

Dry eye disease and ocular surface characteristics in patients with keratoconus

Impact of unilateral trigeminal neuralgia on bilateral ocular surface alterations

A Study On Conjunctival Impression Cytology in Patients with Early Multiple Sclerosis

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