The use ofp-fluorophenylalanine with ‘master strains’ ofAspergillus nidulansfor assigning genes to linkage groups

Abstract

Four ‘master’ strains of Aspergillus nidulans, three of which are marked on each of the eight chromosomes, are used for assigning genes of unknown location to linkage groups. In this technique a heterozygous diploid is synthesized between one ‘master’ strain and a strain carrying an unlocated marker. This diploid is haploidized by growth on p-fluorophenylalanine, and the haploids thus obtained are classified for recombination between the unlocated marker and each of the markers of the ‘master’ strains. The marker or markers of the ‘master’ strain which show no recombination with the unlocated marker identify the chromosome pair on which the latter is located. One example is presented for each of the four ‘master’ strains.

Transcriptional Control of Gluconeogenesis in Aspergillus nidulans

“…Therefore, a diploid between an acuN356 strain and the strain MSF with each linkage group genetically marked was constructed and subjected to haploidization analysis (McCully and Forbes 1965). This revealed that the acuN356 strain contained a translocation event involving linkage groups I and V. Southern blot analysis of HindIII-and HaeIII-digested acuN356 DNA, using cloned acuN DNA as a probe, localized a translocation breakpoint to the 59 region of acuN $150-300 bp upstream of the predicted enolase ATG (Figure 1 and data not shown).…”

Investigation of the het genes that control heterokaryon incompatibility between members of heterokaryon-compatibility (h-c) groups A and G1 of Aspergillus nidulans

“…Linkage was also indicated for acrA withpA, acrA with lacA, and SA with riboB. It is unlikely that linkage is involved, though, as haploidization should lead to the assortment of complete linkage groups (McCully & Forbes, 1965). Mitotic recombination should only disrupt such linkages in approximately 1% of haploidization events (Kafer, 1961(Kafer, , 1977.…”

Amide Utilization in Aspergillus nidulans: Evidence for a Third Amidase Enzyme

“…Haploidization analysis (McCully & Forbes, 1965) showed that bzuAI was located in linkage group IV, and approximately 22 % recombination with mauAz was detected in sexual crosses. Strains containing gmdAI grew poorly on benzamide, phenylacetamide and some other amides as sole nitrogen sources (see below).…”