“…Pre-treatments can be grouped into biological, thermal, mechanical, chemical as well as different combination of these (Appels et al, 2008;Barjenbruch and Kopplow, 2003;Bougrier et al, 2006;Carrère et al, 2010;Kavitha et al, 2014;Kim et al, 2003;Nagare et al, 2008;. In the wide scenario of lysis technologies, mechanical and thermal pretreatments are the most common systems available on the market, working in full scale plants with good results in terms of COD solubilization and AD performance improvement (Bougrier et al, 2008;Choi et al, 2006;Climent et al, 2007;Dohányos et al, 1997Dohányos et al, , 2004Ferrer et al, 2008;Grübel and Machnicka, 2009;Kampas et al, 2007;Machnicka et al, 2009;Zábranská et al, 2006). Soluble COD (SCOD), that is the amount of COD in the liquid phase (after 0.45 lm filtration), is a parameter of easy analytic determination strictly related to sludge degradability.…”