The use of hairless (IAF/HA-HO) guinea pigs for the determination of delayed-type hypersensitivity to tuberculin

Cobb, Angela; Moore, LeeTerry; Godfrey, Henry P.; Gordonov, Natalia; Heilman, Edward; Sizemore, Robert C.

doi:10.1016/s1567-5769(00)00013-8

Cited by 5 publications

(6 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As previously observed, all skin biopsies from hairless animals showed follicular units devoid of normal hair shafts with follicular infundibula containing loosely packed cornified cells and small fragments of compact cornified cells, possibly representing remnants of poorly formed hair shafts [13].…”

Section: Histopathology Of Peptide-modulated Dth Responses Elicited Bsupporting

confidence: 83%

“…Guinea pigs were sensitized by subcutaneous injection in the intranuchal region with 0.5 ml of Freund's complete adjuvant containing 0.5 mg of heat-killed mixed strains of M. tuberculosis and boosted at 6 and 8 weeks by an intradermal injection of 500 ng of PPD in 0.1 ml of sterile phosphate buffered saline (PBS), pH 7.2 [13]. To test the effect of the peptides on DTH responses, a suboptimal dose of PPD (20-125 ng, depending on the skin test reactivity of the animal) was mixed with various doses of peptide or amino acid in sterile PBS and injected intradermally in the flank.…”

Section: Sensitization and Skin Testingmentioning

confidence: 99%

“…Biopsies were divided into two and fixed in neutral formalin or 1% glutaraldehyde. Fixed tissue was embedded in paraffin, sections cut using standard histotechnological techniques, and stained with either hematoxylin and eosin or with Giemsa stain [13]. Results are based on the examination of coded sections from two independent experiments by two separate observers (E.H., H.P.G.…”

Section: Histological Analysis Of Dth Reactionsmentioning

confidence: 99%

“…In a previous study, we demonstrated that hairless guinea pigs (IAF/HA-HO) immunized with Freund's complete adjuvant containing Mycobacterium tuberculosis and repeatedly skin tested with purified protein derivative of tuberculin (PPD) displayed high levels of stable DTH to PPD similar to those seen in Hartley guinea pigs [13]. The advantage of this model is that potentially confounding skin irritation due to removal of hair can be avoided, making quantitation of immunoenhancing and immunosuppressing materials in the skin more reproducible.…”

Section: Introductionmentioning

confidence: 96%

See 3 more Smart Citations

Modulation of Delayed-Type Hypersensitivity Responses in Hairless Guinea Pigs by Peptides Derived from Enkephalin

Sizemore

Piva²,

Moore³

et al. 2004

Neuroimmunomodulation

Self Cite

View full text Add to dashboard Cite

Although opioid peptides such as methionine (met)-enkephalin have been previously shown to enhance or suppress immune responses, few studies in animal models have addressed the immunomodulatory activity of their metabolic derivatives. Hairless (IAF/HA-HO) guinea pigs immunized with Freund’s complete adjuvant containing Mycobacterium tuberculosis and repeatedly skin tested with purified protein derivative of tuberculin (PPD) display high levels of stable delayed-type hypersensitivity (DTH) to PPD. Met-enkephalin (YGGFM) and two of its metabolites (YGG, YG) enhanced and accelerated PPD-elicited DTH inflammatory reactions when injected together with elicitor in these animals. At 24 h, 5 × 10^–3 pmol met-enkephalin significantly enhanced DTH responses by 30% over PPD alone, while 5 × 10^–5 pmol of YGG and 5 × 10^–9 pmol of YG significantly enhanced these responses by 62 and 32%, respectively. At much higher doses (5 × 10³ pmol), met-enkephalin and its metabolites significantly suppressed DTH reactions by 25–32%. Tyrosine and glycine had no effect on PPD-elicited DTH. All DTH reactions (control, enhanced, suppressed) displayed typical perivascular mononuclear cell infiltrates. We conclude that the immunoactivity of met-enkephalin resides in its first two amino acids and suggest that cleavage of enkephalin molecules to YG occurs in serum and/or on the cell surface.

show abstract

Section: Histopathology Of Peptide-modulated Dth Responses Elicited Bsupporting

confidence: 83%

Section: Sensitization and Skin Testingmentioning

confidence: 99%

Section: Histological Analysis Of Dth Reactionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Modulation of Delayed-Type Hypersensitivity Responses in Hairless Guinea Pigs by Peptides Derived from Enkephalin

Sizemore

Piva²,

Moore³

et al. 2004

Neuroimmunomodulation

Self Cite

View full text Add to dashboard Cite

show abstract

“…and not more then 200% of the estimated potency, and the estimated potency not less than 75% and not more than 133%, and not less than 66% and not more than 150%, of the stated potency of 20,000 IU/ml for avian and bovine tuberculin, respectively (European Commission, 1964). To reduce A c c e p t e d M a n u s c r i p t 13 experimentally induced skin reactions, which can interfere with the bio-assay, Cobb et al (2001) propose the use of hairless guinea pigs. As a quality control measure on a number of occasions annually Ireland routinely assays the potency of a selection of the normal tuberculin supplied for use in bovines naturally infected with M. bovis.…”

Section: Discussionmentioning

confidence: 99%